دورية أكاديمية
First mitochondrial and nuclear DNA sequences of Lamanema chavezi (Nematoda: Molineidae): Novel findings to improve its identification in feces from South American camelids.
| العنوان: | First mitochondrial and nuclear DNA sequences of Lamanema chavezi (Nematoda: Molineidae): Novel findings to improve its identification in feces from South American camelids. |
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| المؤلفون: | Petrigh RS; CONICET - Laboratorio de Parasitología de Sitios Arqueológicos, Universidad Nacional de Mar del Plata, Calle Funes 3350, Mar del Plata 7600, Buenos Aires, Argentina. Electronic address: rpetrigh@mdp.edu.ar., Cafrune MM; Instituto Nacional de Tecnología Agropecuaria (INTA), CIAP, Instituto de Investigación Animal del Chaco Semiárido, Área de Investigación en Salud Animal - E.E.A. Salta, Ruta Nacional 68 - km 172, A4403 Cerrillos, Salta, Argentina., Fugassa MH; CONICET - Laboratorio de Parasitología de Sitios Arqueológicos, Universidad Nacional de Mar del Plata, Calle Funes 3350, Mar del Plata 7600, Buenos Aires, Argentina. |
| المصدر: | Parasitology international [Parasitol Int] 2019 Feb; Vol. 68 (1), pp. 60-62. Date of Electronic Publication: 2018 Oct 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9708549 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-0329 (Electronic) Linking ISSN: 13835769 NLM ISO Abbreviation: Parasitol Int Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier, c1997- |
| مواضيع طبية MeSH: | Camelids, New World/*parasitology , DNA, Mitochondrial/*genetics , Feces/*parasitology , Molineoidae/*genetics , Nuclear Proteins/*genetics , Strongylida Infections/*veterinary, Animals ; Animals, Domestic ; Argentina/epidemiology ; DNA, Helminth ; Female ; Genome, Helminth ; Molineoidae/isolation & purification ; Parasite Egg Count ; Polymerase Chain Reaction ; Sequence Analysis, DNA ; Strongylida Infections/epidemiology ; Strongylida Infections/parasitology |
| مستخلص: | Lamanema chavezi (Family Molineidae) is a parasitic nematode of South American camelids (SACs). A few studies have reported this parasite in SACs, mainly in domestic camelid species (llama and alpaca). Parasite identification by means of copro-parasitological methods is non-invasive and allows performing epidemiological studies. However, egg misidentification and difficulty to culture third-stage larvae do not allow identifying nematodes to species level. In contrast, molecular tools allow identifying eggs of gastrointestinal nematodes more accurately. However, the little genomic information available in databases for some species prevents an accurate diagnosis. In the present work, L. chavezi females present in feces of llamas from northwestern Argentina were molecularly characterized to obtain genomic information and improve parasitological diagnosis of L. chavezi-like eggs present in guanaco feces from southeastern Argentina. An 833-bp fragment of nuclear ribosomal DNA (rDNA) and a 434-bp fragment of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene from both L. chavezi females and eggs were amplified and sequenced. Comparison between sequences from females and eggs showed 99-99.6% identity to rDNA and 99.5-96.1% to the cox1 gene fragments, confirming egg morphological assignment. A higher divergence between sequences was observed in the cox1 fragment, with a maximum variation of 3.9%. The examination of eggs found in guanaco feces from southeastern Argentina and their specific molecular identification represent the first record for this host in Argentine Patagonia and contribute to improving the diagnosis of gastrointestinal nematodes in SACs, mainly in wild camelids. (Copyright © 2018 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Lama guanicoe; Molecular identification; Molineid eggs; Patagonia; Wildlife conservation |
| المشرفين على المادة: | 0 (DNA, Helminth) 0 (DNA, Mitochondrial) 0 (Nuclear Proteins) |
| تواريخ الأحداث: | Date Created: 20181021 Date Completed: 20190130 Latest Revision: 20190130 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.parint.2018.10.007 |
| PMID: | 30342118 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1873-0329 |
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| DOI: | 10.1016/j.parint.2018.10.007 |