دورية أكاديمية
Variations of the lung microbiome and immune response in mechanically ventilated surgical patients.
العنوان: | Variations of the lung microbiome and immune response in mechanically ventilated surgical patients. |
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المؤلفون: | Huebinger RM; Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America., Smith AD; Department of Microbiology, Immunology, and Genetics, University of North Texas Health Science Center, Fort Worth, Texas, United States of America., Zhang Y; Department of Microbiology, Immunology, and Genetics, University of North Texas Health Science Center, Fort Worth, Texas, United States of America., Monson NL; Department of Neurology and Neurotherapeutics, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.; Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America., Ireland SJ; Department of Neurology and Neurotherapeutics, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America., Barber RC; Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, Fort Worth, Texas, United States of America., Kubasiak JC; Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America., Minshall CT; Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America., Minei JP; Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America., Wolf SE; Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America., Allen MS; Department of Microbiology, Immunology, and Genetics, University of North Texas Health Science Center, Fort Worth, Texas, United States of America. |
المصدر: | PloS one [PLoS One] 2018 Oct 24; Vol. 13 (10), pp. e0205788. Date of Electronic Publication: 2018 Oct 24 (Print Publication: 2018). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: San Francisco, CA : Public Library of Science |
مواضيع طبية MeSH: | Bacteria/*immunology , Lung/*microbiology , Microbiota/*immunology , Pneumonia, Ventilator-Associated/*immunology , Respiration, Artificial/*adverse effects, Adult ; Aged ; Bacteria/genetics ; Bacteria/isolation & purification ; Bronchoalveolar Lavage Fluid/microbiology ; Cytokines/immunology ; Cytokines/metabolism ; DNA, Bacterial/isolation & purification ; Female ; Humans ; Intensive Care Units ; Lung/immunology ; Male ; Middle Aged ; Pneumonia, Ventilator-Associated/microbiology ; RNA, Ribosomal, 16S/genetics ; Respiration, Artificial/methods |
مستخلص: | Mechanically ventilated surgical patients have a variety of bacterial flora that are often undetectable by traditional culture methods. The source of infection in many of these patients remains unclear. To address this clinical problem, the microbiome profile and host inflammatory response in bronchoalveolar lavage samples from the surgical intensive care unit were examined relative to clinical pathology diagnoses. The hypothesis was tested that clinical diagnosis of respiratory tract flora were similar to culture positive lavage samples in both microbiome and inflammatory profile. Bronchoalveolar lavage samples were collected in the surgical intensive care unit as standard of care for intubated individuals with a clinical pulmonary infection score of >6 or who were expected to be intubated for >48 hours. Cytokine analysis was conducted with the Bioplex Pro Human Th17 cytokine panel. The microbiome of the samples was sequenced for the 16S rRNA region using the Ion Torrent. Microbiome diversity analysis showed the culture-positive samples had the lowest levels of diversity and culture negative with the highest based upon the Shannon-Wiener index (culture positive: 0.77 ± 0.36, respiratory tract flora: 2.06 ± 0.73, culture negative: 3.97 ± 0.65). Culture-negative samples were not dominated by a single bacterial genera. Lavages classified as respiratory tract flora were more similar to the culture-positive in the microbiome profile. A comparison of cytokine expression between groups showed increased levels of cytokines (IFN-g, IL-17F, IL-1B, IL-31, TNF-a) in culture-positive and respiratory tract flora groups. Culture-positive samples exhibited a more robust immune response and reduced diversity of bacterial genera. Lower cytokine levels in culture-negative samples, despite a greater number of bacterial species, suggest a resident nonpathogenic bacterial community may be indicative of a normal pulmonary environment. Respiratory tract flora samples were most similar to the culture-positive samples and may warrant classification as culture-positive when considering clinical treatment. Competing Interests: The authors have declared that no competing interests exist. |
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معلومات مُعتمدة: | T32 AI005284 United States AI NIAID NIH HHS |
المشرفين على المادة: | 0 (Cytokines) 0 (DNA, Bacterial) 0 (RNA, Ribosomal, 16S) |
تواريخ الأحداث: | Date Created: 20181026 Date Completed: 20190405 Latest Revision: 20240808 |
رمز التحديث: | 20240808 |
مُعرف محوري في PubMed: | PMC6200244 |
DOI: | 10.1371/journal.pone.0205788 |
PMID: | 30356313 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1932-6203 |
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DOI: | 10.1371/journal.pone.0205788 |