دورية أكاديمية
أعرض في EDS

Variations of the lung microbiome and immune response in mechanically ventilated surgical patients.

التفاصيل البيبلوغرافية
العنوان: Variations of the lung microbiome and immune response in mechanically ventilated surgical patients.
المؤلفون: Huebinger RM; Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America., Smith AD; Department of Microbiology, Immunology, and Genetics, University of North Texas Health Science Center, Fort Worth, Texas, United States of America., Zhang Y; Department of Microbiology, Immunology, and Genetics, University of North Texas Health Science Center, Fort Worth, Texas, United States of America., Monson NL; Department of Neurology and Neurotherapeutics, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.; Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America., Ireland SJ; Department of Neurology and Neurotherapeutics, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America., Barber RC; Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, Fort Worth, Texas, United States of America., Kubasiak JC; Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America., Minshall CT; Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America., Minei JP; Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America., Wolf SE; Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America., Allen MS; Department of Microbiology, Immunology, and Genetics, University of North Texas Health Science Center, Fort Worth, Texas, United States of America.
المصدر: PloS one [PLoS One] 2018 Oct 24; Vol. 13 (10), pp. e0205788. Date of Electronic Publication: 2018 Oct 24 (Print Publication: 2018).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Bacteria/*immunology , Lung/*microbiology , Microbiota/*immunology , Pneumonia, Ventilator-Associated/*immunology , Respiration, Artificial/*adverse effects, Adult ; Aged ; Bacteria/genetics ; Bacteria/isolation & purification ; Bronchoalveolar Lavage Fluid/microbiology ; Cytokines/immunology ; Cytokines/metabolism ; DNA, Bacterial/isolation & purification ; Female ; Humans ; Intensive Care Units ; Lung/immunology ; Male ; Middle Aged ; Pneumonia, Ventilator-Associated/microbiology ; RNA, Ribosomal, 16S/genetics ; Respiration, Artificial/methods
مستخلص: Mechanically ventilated surgical patients have a variety of bacterial flora that are often undetectable by traditional culture methods. The source of infection in many of these patients remains unclear. To address this clinical problem, the microbiome profile and host inflammatory response in bronchoalveolar lavage samples from the surgical intensive care unit were examined relative to clinical pathology diagnoses. The hypothesis was tested that clinical diagnosis of respiratory tract flora were similar to culture positive lavage samples in both microbiome and inflammatory profile. Bronchoalveolar lavage samples were collected in the surgical intensive care unit as standard of care for intubated individuals with a clinical pulmonary infection score of >6 or who were expected to be intubated for >48 hours. Cytokine analysis was conducted with the Bioplex Pro Human Th17 cytokine panel. The microbiome of the samples was sequenced for the 16S rRNA region using the Ion Torrent. Microbiome diversity analysis showed the culture-positive samples had the lowest levels of diversity and culture negative with the highest based upon the Shannon-Wiener index (culture positive: 0.77 ± 0.36, respiratory tract flora: 2.06 ± 0.73, culture negative: 3.97 ± 0.65). Culture-negative samples were not dominated by a single bacterial genera. Lavages classified as respiratory tract flora were more similar to the culture-positive in the microbiome profile. A comparison of cytokine expression between groups showed increased levels of cytokines (IFN-g, IL-17F, IL-1B, IL-31, TNF-a) in culture-positive and respiratory tract flora groups. Culture-positive samples exhibited a more robust immune response and reduced diversity of bacterial genera. Lower cytokine levels in culture-negative samples, despite a greater number of bacterial species, suggest a resident nonpathogenic bacterial community may be indicative of a normal pulmonary environment. Respiratory tract flora samples were most similar to the culture-positive samples and may warrant classification as culture-positive when considering clinical treatment.
Competing Interests: The authors have declared that no competing interests exist.
References: PLoS One. 2016 Apr 14;11(4):e0152724. (PMID: 27078029)
Curr Opin Infect Dis. 2013 Apr;26(2):140-50. (PMID: 23411419)
Thorax. 2015 Jan;70(1):41-7. (PMID: 25298325)
Chest. 2015 Jun;147(6):1494-1502. (PMID: 25474571)
BMC Pulm Med. 2016 Dec 1;16(1):170. (PMID: 27905908)
J Trauma. 2006 May;60(5):1106-13; discussion 1113. (PMID: 16688078)
Intensive Care Med. 2015 Jan;41(1):34-48. (PMID: 25427866)
ISME J. 2016 Jan;10(1):97-108. (PMID: 26151645)
Thorax. 2016 Sep;71(9):795-803. (PMID: 27146202)
Respir Care. 2016 Mar;61(3):269-76. (PMID: 26556896)
J Clin Microbiol. 2014 Oct;52(10):3597-604. (PMID: 25078907)
Chest. 2002 Aug;122(2):662-8. (PMID: 12171848)
Int Forum Allergy Rhinol. 2017 Mar;7(3):230-239. (PMID: 27879060)
PLoS One. 2011 Feb 22;6(2):e16384. (PMID: 21364979)
Bioinformatics. 2011 Aug 15;27(16):2194-200. (PMID: 21700674)
Surg Infect (Larchmt). 2017 Jul;18(5):558-562. (PMID: 28561600)
Am J Infect Control. 2016 Jun 1;44(6):661-5. (PMID: 26899526)
Thorax. 2017 Nov;72(11):1046-1048. (PMID: 27974525)
PLoS One. 2009 Oct 09;4(10):e7401. (PMID: 19816594)
Intensive Care Med. 2004 Feb;30(2):217-224. (PMID: 14566455)
J Clin Microbiol. 2013 Mar;51(3):740-4. (PMID: 23284021)
J Crit Care. 2017 Apr;38:84-91. (PMID: 27866110)
PLoS One. 2014 Oct 07;9(10):e109686. (PMID: 25289689)
Thorax. 2017 Sep;72(9):803-810. (PMID: 28100714)
J Am Coll Surg. 2012 Apr;214(4):478-86; discussion 486-8. (PMID: 22342787)
BMC Pulm Med. 2015 Aug 12;15:86. (PMID: 26264828)
J Trauma. 1993 Oct;35(4):512-7. (PMID: 8411272)
Appl Environ Microbiol. 2005 Dec;71(12):8228-35. (PMID: 16332807)
J Trauma Acute Care Surg. 2012 May;72(5):1165-73. (PMID: 22673241)
Surg Infect (Larchmt). 2016 Jun;17(3):363-8. (PMID: 26938612)
PLoS One. 2016 Nov 29;11(11):e0166313. (PMID: 27898681)
Respir Care. 2009 Nov;54(11):1453-61. (PMID: 19863828)
Appl Environ Microbiol. 2009 Dec;75(23):7537-41. (PMID: 19801464)
Intensive Care Med. 2004 Jan;30(1):68-74. (PMID: 14634726)
Clin Infect Dis. 2016 Sep 1;63(5):e61-e111. (PMID: 27418577)
Thorax. 2010 Mar;65(3):201-7. (PMID: 19825784)
Shock. 2016 Dec;46(6):649-654. (PMID: 27454385)
J Trauma. 2006 Mar;60(3):523-7; discussion 527-8. (PMID: 16531849)
Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4516-22. (PMID: 20534432)
معلومات مُعتمدة: T32 AI005284 United States AI NIAID NIH HHS
المشرفين على المادة: 0 (Cytokines)
0 (DNA, Bacterial)
0 (RNA, Ribosomal, 16S)
تواريخ الأحداث: Date Created: 20181026 Date Completed: 20190405 Latest Revision: 20240808
رمز التحديث: 20240808
مُعرف محوري في PubMed: PMC6200244
DOI: 10.1371/journal.pone.0205788
PMID: 30356313
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0205788