دورية أكاديمية
أعرض في EDS

Inhibition of Osteocyte Membrane Repair Activity via Dietary Vitamin E Deprivation Impairs Osteocyte Survival.

التفاصيل البيبلوغرافية
العنوان: Inhibition of Osteocyte Membrane Repair Activity via Dietary Vitamin E Deprivation Impairs Osteocyte Survival.
المؤلفون: Hagan ML; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th St, Augusta, GA, 30912, USA., Bahraini A; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th St, Augusta, GA, 30912, USA., Pierce JL; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th St, Augusta, GA, 30912, USA., Bass SM; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th St, Augusta, GA, 30912, USA., Yu K; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th St, Augusta, GA, 30912, USA., Elsayed R; Department of Oral Biology, Augusta University, 1120 15th St, Augusta, GA, USA., Elsalanty M; Department of Oral Biology, Augusta University, 1120 15th St, Augusta, GA, USA., Johnson MH; Department of Neuroscience and Regenerative Medicine, Augusta University, 1120 15th St, Augusta, GA, USA., McNeil A; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th St, Augusta, GA, 30912, USA., McNeil PL; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th St, Augusta, GA, 30912, USA., McGee-Lawrence ME; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th St, Augusta, GA, 30912, USA. mmcgeelawrence@augusta.edu.; Department of Orthopaedic Surgery, Augusta University, 1120 15th St, Augusta, GA, USA. mmcgeelawrence@augusta.edu.
المصدر: Calcified tissue international [Calcif Tissue Int] 2019 Feb; Vol. 104 (2), pp. 224-234. Date of Electronic Publication: 2018 Oct 24.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Springer Verlag Country of Publication: United States NLM ID: 7905481 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0827 (Electronic) Linking ISSN: 0171967X NLM ISO Abbreviation: Calcif Tissue Int Subsets: MEDLINE
أسماء مطبوعة: Publication: New York Ny : Springer Verlag
Original Publication: Berlin, New York, Springer International.
مواضيع طبية MeSH: Cell Membrane/*physiology , Osteocytes/*physiology , Regeneration/*physiology , Vitamin E/*metabolism , Vitamin E Deficiency/*physiopathology, Animals ; Bone Resorption/metabolism ; Cell Membrane/metabolism ; Cell Membrane/pathology ; Cell Membrane Permeability/physiology ; Cell Survival/drug effects ; Male ; Mechanotransduction, Cellular/drug effects ; Mechanotransduction, Cellular/physiology ; Mice ; Osteocytes/metabolism ; Physical Conditioning, Animal/physiology ; Vitamin E/pharmacology ; Vitamin E Deficiency/metabolism ; Weight-Bearing/physiology
مستخلص: Osteocytes experience plasma membrane disruptions (PMD) that initiate mechanotransduction both in vitro and in vivo in response to mechanical loading, suggesting that osteocytes use PMD to sense and adapt to mechanical stimuli. PMD repair is crucial for cell survival; antioxidants (e.g., alpha-tocopherol, also known as Vitamin E) promote repair while reactive oxygen species (ROS), which can accumulate during exercise, inhibit repair. The goal of this study was to determine whether depleting Vitamin E in the diet would impact osteocyte survival and bone adaptation with loading. Male CD-1 mice (3 weeks old) were fed either a regular diet (RD) or Vitamin E-deficient diet (VEDD) for up to 11 weeks. Mice from each dietary group either served as sedentary controls with normal cage activity, or were subjected to treadmill exercise (one bout of exercise or daily exercise for 5 weeks). VEDD-fed mice showed more PMD-affected osteocytes (+ 50%) after a single exercise bout suggesting impaired PMD repair following Vitamin E deprivation. After 5 weeks of daily exercise, VEDD mice failed to show an exercise-induced increase in osteocyte PMD formation, and showed signs of increased osteocytic oxidative stress and impaired osteocyte survival. Surprisingly, exercise-induced increases in cortical bone formation rate were only significant for VEDD-fed mice. This result may be consistent with previous studies in skeletal muscle, where myocyte PMD repair failure (e.g., with muscular dystrophy) initially triggers hypertrophy but later leads to widespread degeneration. In vitro, mechanically wounded MLO-Y4 cells displayed increased post-wounding necrosis (+ 40-fold) in the presence of H 2 O 2 , which could be prevented by Vitamin E pre-treatment. Taken together, our data support the idea that antioxidant-influenced osteocyte membrane repair is a vital aspect of bone mechanosensation in the osteocytic control of PMD-driven bone adaptation.
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معلومات مُعتمدة: P01 AG036675 United States AG NIA NIH HHS
فهرسة مساهمة: Keywords: Alpha-tocopherol; Bone; Mechanical loading; Mechanotransduction; Osteocyte; Skeleton; Vitamin E
المشرفين على المادة: 1406-18-4 (Vitamin E)
تواريخ الأحداث: Date Created: 20181026 Date Completed: 20200609 Latest Revision: 20240715
رمز التحديث: 20240715
مُعرف محوري في PubMed: PMC6452877
DOI: 10.1007/s00223-018-0487-0
PMID: 30357446
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-0827
DOI:10.1007/s00223-018-0487-0