دورية أكاديمية
An automated microfluidic chemiluminescence immunoassay platform for quantitative detection of biomarkers.
| العنوان: | An automated microfluidic chemiluminescence immunoassay platform for quantitative detection of biomarkers. |
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| المؤلفون: | Min X; School of Information Science and Technology, Xiamen University, Xiamen, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China.; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Xiamen University, Xiamen, Fujian, China., Fu D; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China.; School of Public Health, Xiamen University, Xiamen, Fujian, China., Zhang J; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China.; School of Public Health, Xiamen University, Xiamen, Fujian, China., Zeng J; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China., Weng Z; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China.; School of Public Health, Xiamen University, Xiamen, Fujian, China., Chen W; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China., Zhang S; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China.; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Xiamen University, Xiamen, Fujian, China.; School of Public Health, Xiamen University, Xiamen, Fujian, China., Zhang D; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China. zhangdongxu@xmu.edu.cn.; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Xiamen University, Xiamen, Fujian, China. zhangdongxu@xmu.edu.cn.; School of Public Health, Xiamen University, Xiamen, Fujian, China. zhangdongxu@xmu.edu.cn., Ge S; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China. sxge@xmu.edu.cn.; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Xiamen University, Xiamen, Fujian, China. sxge@xmu.edu.cn.; School of Public Health, Xiamen University, Xiamen, Fujian, China. sxge@xmu.edu.cn., Zhang J; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China.; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Xiamen University, Xiamen, Fujian, China.; School of Public Health, Xiamen University, Xiamen, Fujian, China., Xia N; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen, Fujian, China.; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Xiamen University, Xiamen, Fujian, China.; School of Public Health, Xiamen University, Xiamen, Fujian, China. |
| المصدر: | Biomedical microdevices [Biomed Microdevices] 2018 Oct 25; Vol. 20 (4), pp. 91. Date of Electronic Publication: 2018 Oct 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer US Country of Publication: United States NLM ID: 100887374 Publication Model: Electronic Cited Medium: Internet ISSN: 1572-8781 (Electronic) Linking ISSN: 13872176 NLM ISO Abbreviation: Biomed Microdevices Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York : Springer US Original Publication: Boston : Kluwer Academic Publishers, 1998- |
| مواضيع طبية MeSH: | Lab-On-A-Chip Devices*/economics, Biomarkers/*analysis , Immunoassay/*instrumentation , Luminescent Measurements/*instrumentation, Automation ; Costs and Cost Analysis ; Ferritins/analysis |
| مستخلص: | A rapid, sensitive and quantitative biomarker detection platform is of great importance to the small clinic or point-of-care (POC) diagnosis. In this work, we realize that an automated diagnostic platform mainly includes two components: (1) an instrument that can complete all steps of the chemiluminescence immunoassay automatically and (2) an integrated microfluidic chip which is disposable and harmless. In the instrument, we adopt vacuum suction cups which are driven by linear motor to realize a simple, effective and convenient control. The method of acridine esterification chemiluminescence is adopted to achieve a quantitative detection, and a photomultiplier tube is used to detect photons from acridine ester producing in alkaline conditions. We use the laser cutting machine and hot press machine to accomplish the product of microfluidic chips. The automated microfluidics-based system is demonstrated by implementation of a chemiluminescence immunoassay for quantitative detection of ferritin. We observe alinear relationship between CL intensity and the concentration of ferritin from 5.1 to 1300 ng mL -1 and the limit of detection (LoD) is 2.55 ng mL -1 . At the same time, we also used the automated microfluidics-based system to test clinical serum samples. The whole process of chemiluminescence experiment can complete within 45 min. We realize that this lab-on-a-chip chemiluminescence immunoassay platform with features of automation and quantitation provides a promising strategy for POC diagnosis. |
| فهرسة مساهمة: | Keywords: Acridine ester; Chemiluminescence; Ferritin; Microfluidicchip |
| المشرفين على المادة: | 0 (Biomarkers) 9007-73-2 (Ferritins) |
| تواريخ الأحداث: | Date Created: 20181027 Date Completed: 20190529 Latest Revision: 20190529 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s10544-018-0331-3 |
| PMID: | 30361769 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1572-8781 |
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| DOI: | 10.1007/s10544-018-0331-3 |