دورية أكاديمية
Phycocyanin alpha-subunit gene of Anacystis nidulans R2: cloning, nucleotide sequencing and expression in Escherichia coli.
| العنوان: | Phycocyanin alpha-subunit gene of Anacystis nidulans R2: cloning, nucleotide sequencing and expression in Escherichia coli. |
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| المؤلفون: | Lau RH, Alvarado-Urbina G, Lau PC |
| المصدر: | Gene [Gene] 1987; Vol. 52 (1), pp. 21-9. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print Cited Medium: Print ISSN: 0378-1119 (Print) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland, 1976- |
| مواضيع طبية MeSH: | Cloning, Molecular* , Genes* , Transcription, Genetic*, Cyanobacteria/*genetics , Escherichia coli/*genetics , Phycocyanin/*genetics , Pigments, Biological/*genetics, Amino Acid Sequence ; Base Sequence ; DNA Restriction Enzymes ; Macromolecular Substances |
| مستخلص: | The cloning and nucleotide sequence determination of the Anacystis nidulans R2 phycocyanin (PC) alpha-subunit gene are described. A 3.0-kb PstI fragment of Anacystis nidulans R2 genomic DNA cloned in plasmid pUC8 was found to hybridize with a heptadecameric oligodeoxynucleotide probe. Sequencing using synthetic primers revealed the presence of the PC alpha-subunit gene and the 3' proximal end of the beta-subunit gene. The alpha-gene is separated from the upstream beta-gene by a spacer length of 51 bp. The deduced amino acid (aa) sequence of the alpha-subunit protein is identical, except for 5 aa, to that of A. nidulans 6301 and is highly homologous (77%) to that reported for Agmenellum quadruplicatum PR6. The 16-kDa alpha-subunit protein, detected by immunoadsorption, was fortuitously expressed in Escherichia coli from the lacZ promoter of the cloning vehicle pUC8. |
| سلسلة جزيئية: | GENBANK M16325 |
| المشرفين على المادة: | 0 (Macromolecular Substances) 0 (Pigments, Biological) 11016-15-2 (Phycocyanin) EC 3.1.21.- (DNA Restriction Enzymes) |
| تواريخ الأحداث: | Date Created: 19870101 Date Completed: 19870810 Latest Revision: 20190707 |
| رمز التحديث: | 20221208 |
| DOI: | 10.1016/0378-1119(87)90391-x |
| PMID: | 3036657 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0378-1119 |
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| DOI: | 10.1016/0378-1119(87)90391-x |