دورية أكاديمية
Evaluation of cytotoxicity and antimicrobial activity of an injectable bone substitute of carrageenan and nano hydroxyapatite.
| العنوان: | Evaluation of cytotoxicity and antimicrobial activity of an injectable bone substitute of carrageenan and nano hydroxyapatite. |
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| المؤلفون: | González Ocampo JI; Biomaterials Research Group, Bioengineering Program, Engineering Faculty, University of Antioquia, Medellin, Colombia., Bassous N; Department of Chemical Engineering, Northeastern University, Boston, Massachusetts., Ossa Orozco CP; Biomaterials Research Group, Bioengineering Program, Engineering Faculty, University of Antioquia, Medellin, Colombia., Webster TJ; Department of Chemical Engineering, Northeastern University, Boston, Massachusetts. |
| المصدر: | Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2018 Nov; Vol. 106 (11), pp. 2984-2993. Date of Electronic Publication: 2018 Oct 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Country of Publication: United States NLM ID: 101234237 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1552-4965 (Electronic) Linking ISSN: 15493296 NLM ISO Abbreviation: J Biomed Mater Res A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Hoboken, NJ : John Wiley & Sons, c2002- |
| مواضيع طبية MeSH: | Anti-Bacterial Agents/*pharmacology , Bone Substitutes/*pharmacology , Carrageenan/*pharmacology , Durapatite/*pharmacology , Osteoblasts/*drug effects, Anti-Bacterial Agents/administration & dosage ; Anti-Bacterial Agents/chemistry ; Bacteria/drug effects ; Bacteria/growth & development ; Bacterial Infections/prevention & control ; Bone Substitutes/administration & dosage ; Bone Substitutes/chemistry ; Carrageenan/administration & dosage ; Carrageenan/chemistry ; Cell Line ; Durapatite/administration & dosage ; Durapatite/chemistry ; Humans ; Injections ; Nanotubes/chemistry ; Nanotubes/ultrastructure ; Osteoblasts/cytology |
| مستخلص: | A successful post-surgical implant is associated with accelerated recovery periods, involving the efficient regeneration of lost or non-viable tissue and a reduction in microbial growth. Alternatively, the long-term success of an implant is guided by the selection of an engineered biomimetic material that is biocompatible, non-biodegradable, and stable at the site of implantation, without invoking any non-essential or undesirable biological responses. The potential for developing an injectable bone substitute (IBS) was investigated here. In particular, carrageenan (CG) and nano-hydroxyapatite (nHA) injectable composites were fabricated by chemical cross-linking, and the in vitro behavior of mammalian cells and bacteria on the IBS surface structures were evaluated. Formulations consisting of 1%, 1.5%, and 2.5% CG and 60% nHA by weight were then evaluated for their interactions with human osteoblasts (or bone forming cells). MTS viability testing indicated that osteoblast adhesion and viability on the IBS were excellent and uniform among various formulation types. Bacteria assays were also performed to assess antimicrobial functions on the CG/nHA composite against both Gram-negative and Gram-positive strains. A higher CG content, as found in some samples, correlated with improved Pseudomonas aeruginosa growth inhibition, although other bacteria strains appeared unaffected by the IBS. In summary, this study highlights CG/nHA composites as innovative biomaterials that should be further studied for reduced bacteria activity and promoted osteoblast responses which was achieved without using pharmaceutical drugs. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2984-2993, 2018. (© 2018 Wiley Periodicals, Inc.) |
| فهرسة مساهمة: | Keywords: antimicrobial; bacteria; bone substitute; carrageenan; hydroxyapatite; nanophase |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Bone Substitutes) 9000-07-1 (Carrageenan) 91D9GV0Z28 (Durapatite) |
| تواريخ الأحداث: | Date Created: 20181028 Date Completed: 20191030 Latest Revision: 20191030 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1002/jbm.a.36488 |
| PMID: | 30367558 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1552-4965 |
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| DOI: | 10.1002/jbm.a.36488 |