دورية أكاديمية
أعرض في EDS

SNW1, a Novel Transcriptional Regulator of the NF-κB Pathway.

التفاصيل البيبلوغرافية
العنوان: SNW1, a Novel Transcriptional Regulator of the NF-κB Pathway.
المؤلفون: Verma S; Salk Institute for Biological Studies, La Jolla, California, USA., De Jesus P; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, USA., Chanda SK; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, USA schanda@sbpdiscovery.org imohanv1@gmail.com., Verma IM; Salk Institute for Biological Studies, La Jolla, California, USA schanda@sbpdiscovery.org imohanv1@gmail.com.
المصدر: Molecular and cellular biology [Mol Cell Biol] 2019 Jan 16; Vol. 39 (3). Date of Electronic Publication: 2019 Jan 16 (Print Publication: 2019).
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 8109087 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1098-5549 (Electronic) Linking ISSN: 02707306 NLM ISO Abbreviation: Mol Cell Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2023- : [Philadelphia] : Taylor & Francis
Original Publication: [Washington, D.C.] : American Society for Microbiology, [c1981-
مواضيع طبية MeSH: NF-kappa B/*metabolism , Nuclear Receptor Coactivators/*genetics , Nuclear Receptor Coactivators/*metabolism, Gene Expression Regulation ; Humans ; Macrophages/metabolism ; Positive Transcriptional Elongation Factor B/metabolism ; Promoter Regions, Genetic ; Signal Transduction ; THP-1 Cells ; Transcription Factor RelA/metabolism ; Transcription Factors/metabolism ; Transcription, Genetic ; Transcriptional Activation ; Tumor Necrosis Factor-alpha/metabolism
مستخلص: The nuclear factor kappa B (NF-κB) family of transcription factors plays a central role in coordinating the expression of genes that control inflammation, immune responses, cell proliferation, and a variety of other biological processes. In an attempt to identify novel regulators of this pathway, we performed whole-genome RNA interference (RNAi) screens in physiologically relevant human macrophages in response to lipopolysaccharide and tumor necrosis factor alpha (TNF-α). The top hit was SNW1, a splicing factor and transcriptional coactivator. SNW1 does not regulate the cytoplasmic components of the NF-κB pathway but complexes with the NF-κB heterodimer in the nucleus for transcriptional activation. We show that SNW1 detaches from its splicing complex (formed with SNRNP200 and SNRNP220) upon NF-κB activation and binds to NF-κB's transcriptional elongation partner p-TEFb. We also show that SNW1 is indispensable for the transcriptional elongation of NF-κB target genes such as the interleukin 8 (IL-8) and TNF genes. SNW1 is a unique protein previously shown to be involved in both splicing and transcription, and in this case, its role involves binding to the NF-κB-p-TEFb complex to facilitate transcriptional elongation of some NF-κB target genes.
(Copyright © 2019 American Society for Microbiology.)
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معلومات مُعتمدة: P30 CA014195 United States CA NCI NIH HHS; R01 AI105184 United States AI NIAID NIH HHS
فهرسة مساهمة: Keywords: NF-κB; RNAi screen; transcriptional regulation
المشرفين على المادة: 0 (NF-kappa B)
0 (Nuclear Receptor Coactivators)
0 (RELA protein, human)
0 (SNW1 protein, human)
0 (Transcription Factor RelA)
0 (Transcription Factors)
0 (Tumor Necrosis Factor-alpha)
EC 2.7.11.- (Positive Transcriptional Elongation Factor B)
تواريخ الأحداث: Date Created: 20181107 Date Completed: 20191202 Latest Revision: 20210227
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC6336138
DOI: 10.1128/MCB.00415-18
PMID: 30397075
قاعدة البيانات: MEDLINE
الوصف
تدمد:1098-5549
DOI:10.1128/MCB.00415-18