دورية أكاديمية
أعرض في EDS

Expression and localisation of thymosin beta-4 in the developing human early fetal heart.

التفاصيل البيبلوغرافية
العنوان: Expression and localisation of thymosin beta-4 in the developing human early fetal heart.
المؤلفون: Saunders V; Institute for Developmental Science, Faculty of Medicine, University of Southampton, Southampton, United Kingdom., Dewing JM; Institute for Developmental Science, Faculty of Medicine, University of Southampton, Southampton, United Kingdom., Sanchez-Elsner T; Academic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, United Kingdom., Wilson DI; Institute for Developmental Science, Faculty of Medicine, University of Southampton, Southampton, United Kingdom.
المصدر: PloS one [PLoS One] 2018 Nov 09; Vol. 13 (11), pp. e0207248. Date of Electronic Publication: 2018 Nov 09 (Print Publication: 2018).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Fetal Heart/*metabolism , Thymosin/*genetics , Thymosin/*metabolism, Coronary Vessels/cytology ; Coronary Vessels/embryology ; Coronary Vessels/metabolism ; Endothelial Cells/cytology ; Endothelial Cells/metabolism ; Female ; Fetal Heart/cytology ; Fetal Heart/embryology ; Gene Expression Regulation, Developmental ; Heart Ventricles/cytology ; Heart Ventricles/embryology ; Heart Ventricles/metabolism ; Humans ; Immunohistochemistry ; Myocytes, Cardiac/cytology ; Myocytes, Cardiac/metabolism ; Pregnancy ; RNA, Messenger/genetics ; RNA, Messenger/metabolism
مستخلص: Background: The objective of this study was to investigate the expression and localisation of thymosin β4 (Tβ4) in the developing human heart. Tβ4 is a cardioprotective protein which may have therapeutic potential. While Tβ4 is an endogenously produced protein with known importance during development, its role within the developing human heart is not fully understood. Elucidating the localisation of Tβ4 within the developing heart will help in understanding its role during cardiac development and is crucial for understanding its potential for cardioprotection and repair in the adult heart.
Methods: Expression of Tβ4 mRNA in the early fetal human heart was assessed by PCR using both ventricular and atrial tissue. Fluorescence immunohistochemistry was used to assess the localisation of Tβ4 in sections of early fetal human heart. Co-staining with CD31, an endothelial cell marker, and with myosin heavy chain, a cardiomyocyte marker, was used to determine whether Tβ4 is localised to these cell types within the early fetal human heart.
Results: Tβ4 mRNA was found to be expressed in both the atria and the ventricles of the early fetal human heart. Tβ4 protein was found to be primarily localised to CD31-expressing endothelial cells and the endocardium as well as being present in the epicardium. Tβ4-associated fluorescence was greater in the compact layer of the myocardial wall and the interventricular septum than in the trabecular layer of the myocardium.
Conclusions: The data presented illustrates expression of Tβ4 in the developing human heart and demonstrates for the first time that Tβ4 in the human heart is primarily localised to endothelial cells of the cardiac microvasculature and coronary vessels as-well as to the endothelial-like cells of the endocardium and to the epicardium.
Competing Interests: The authors have declared that no competing interests exist.
References: Cardiovasc Res. 1998 Apr;38(1):25-53. (PMID: 9683906)
Ann N Y Acad Sci. 2010 Apr;1194:105-11. (PMID: 20536456)
J Invest Dermatol. 1999 Sep;113(3):364-8. (PMID: 10469335)
FASEB J. 1997 May;11(6):474-81. (PMID: 9194528)
Appl Immunohistochem Mol Morphol. 2011 Mar;19(2):160-7. (PMID: 20975530)
Nature. 2007 Jan 11;445(7124):177-82. (PMID: 17108969)
Exp Eye Res. 2006 Sep;83(3):502-7. (PMID: 16630613)
PLoS One. 2013 Sep 24;8(9):e75580. (PMID: 24086577)
Nat Med. 1999 Dec;5(12):1424-7. (PMID: 10581087)
Nature. 2004 Nov 25;432(7016):466-72. (PMID: 15565145)
Circ Res. 2012 Feb 3;110(3):456-64. (PMID: 22158707)
Am J Physiol. 1998 May;274(5 Pt 2):H1620-6. (PMID: 9612372)
FASEB J. 2011 Jun;25(6):1815-26. (PMID: 21343177)
Circulation. 2008 Apr 29;117(17):2232-40. (PMID: 18427126)
Invest Ophthalmol Vis Sci. 2004 Apr;45(4):1095-100. (PMID: 15037574)
Cold Spring Harb Perspect Med. 2013 Oct 01;3(10):a013847. (PMID: 24086063)
J Nanobiotechnology. 2014 Mar 24;12:10. (PMID: 24661328)
Exp Eye Res. 2001 May;72(5):605-8. (PMID: 11311052)
Invest Ophthalmol Vis Sci. 2005 Jul;46(7):2388-95. (PMID: 15980226)
Circ Res. 2013 Feb 1;112(3):e25-8. (PMID: 23371905)
Circ Res. 2012 Aug 3;111(4):e89-102. (PMID: 22723298)
Exp Eye Res. 2002 Feb;74(2):293-9. (PMID: 11950239)
Angiogenesis. 1999;3(2):125-35. (PMID: 14517430)
معلومات مُعتمدة: United Kingdom Wellcome Trust; 099175/2/12/2 United Kingdom Wellcome Trust; G1000406 United Kingdom Medical Research Council
المشرفين على المادة: 0 (RNA, Messenger)
549LM7U24W (thymosin beta(4))
61512-21-8 (Thymosin)
تواريخ الأحداث: Date Created: 20181110 Date Completed: 20190422 Latest Revision: 20190422
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6226193
DOI: 10.1371/journal.pone.0207248
PMID: 30412598
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0207248