دورية أكاديمية
Self-Assembly of the RZZ Complex into Filaments Drives Kinetochore Expansion in the Absence of Microtubule Attachment.
| العنوان: | Self-Assembly of the RZZ Complex into Filaments Drives Kinetochore Expansion in the Absence of Microtubule Attachment. |
|---|---|
| المؤلفون: | Pereira C; Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, 4200-135 Porto, Portugal; Instituto de Investigação e Inovação em Saúde (i3S), Universidade do Porto, 4200-135 Porto, Portugal., Reis RM; Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, 4200-135 Porto, Portugal; Instituto de Investigação e Inovação em Saúde (i3S), Universidade do Porto, 4200-135 Porto, Portugal., Gama JB; Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, 4200-135 Porto, Portugal; Instituto de Investigação e Inovação em Saúde (i3S), Universidade do Porto, 4200-135 Porto, Portugal., Celestino R; Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, 4200-135 Porto, Portugal; Instituto de Investigação e Inovação em Saúde (i3S), Universidade do Porto, 4200-135 Porto, Portugal., Cheerambathur DK; Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA., Carvalho AX; Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, 4200-135 Porto, Portugal; Instituto de Investigação e Inovação em Saúde (i3S), Universidade do Porto, 4200-135 Porto, Portugal., Gassmann R; Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, 4200-135 Porto, Portugal; Instituto de Investigação e Inovação em Saúde (i3S), Universidade do Porto, 4200-135 Porto, Portugal. Electronic address: rgassmann@ibmc.up.pt. |
| المصدر: | Current biology : CB [Curr Biol] 2018 Nov 05; Vol. 28 (21), pp. 3408-3421.e8. Date of Electronic Publication: 2018 Oct 25. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: England NLM ID: 9107782 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0445 (Electronic) Linking ISSN: 09609822 NLM ISO Abbreviation: Curr Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge, MA : Cell Press Original Publication: London, UK : Current Biology Ltd., c1991- |
| مواضيع طبية MeSH: | Caenorhabditis elegans/*metabolism , Kinetochores/*metabolism , Microtubules/*metabolism, Animals ; Caenorhabditis elegans/embryology ; Cell Cycle Proteins/genetics ; Cell Cycle Proteins/metabolism ; Dyneins/metabolism ; Humans ; Mad2 Proteins/genetics ; Mad2 Proteins/metabolism ; Nuclear Proteins/genetics ; Nuclear Proteins/metabolism |
| مستخلص: | The kinetochore is a dynamic multi-protein assembly that forms on each sister chromatid and interacts with microtubules of the mitotic spindle to drive chromosome segregation. In animals, kinetochores without attached microtubules expand their outermost layer into crescent and ring shapes to promote microtubule capture and spindle assembly checkpoint (SAC) signaling. Kinetochore expansion is an example of protein co-polymerization, but the mechanism is not understood. Here, we present evidence that kinetochore expansion is driven by oligomerization of the Rod-Zw10-Zwilch (RZZ) complex, an outer kinetochore component that recruits the motor dynein and the SAC proteins Mad1-Mad2. Depletion of ROD in human cells suppresses kinetochore expansion, as does depletion of Spindly, the adaptor that connects RZZ to dynein, although dynein itself is dispensable. Expansion is also suppressed by mutating ZWILCH residues implicated in Spindly binding. Conversely, supplying cells with excess ROD facilitates kinetochore expansion under otherwise prohibitive conditions. Using the C. elegans early embryo, we demonstrate that ROD-1 has a concentration-dependent propensity for oligomerizing into micrometer-scale filaments, and we identify the ROD-1 β-propeller as a key regulator of self-assembly. Finally, we show that a minimal ROD-1-Zw10 complex efficiently oligomerizes into filaments in vitro. Our results suggest that RZZ's capacity for oligomerization is harnessed by kinetochores to assemble the expanded outermost domain, in which RZZ filaments serve as recruitment platforms for SAC components and microtubule-binding proteins. Thus, we propose that reversible RZZ self-assembly into filaments underlies the adaptive change in kinetochore size that contributes to chromosome segregation fidelity. (Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| التعليقات: | Comment in: Curr Biol. 2018 Nov 5;28(21):R1250-R1252. (PMID: 30399347) |
| References: | Mol Biol Cell. 2001 Jul;12(7):1995-2009. (PMID: 11451998) Genes Dev. 2010 May;24(9):957-71. (PMID: 20439434) Dev Cell. 2017 Apr 24;41(2):143-156.e6. (PMID: 28441529) J Cell Biol. 2017 Apr 3;216(4):943-960. (PMID: 28320824) J Cell Biol. 2013 Apr 1;201(1):23-32. (PMID: 23530067) Int Rev Cytol. 1982;79:1-58. (PMID: 6185450) Elife. 2017 Jan 10;6:. (PMID: 28072388) J Cell Biol. 2017 Apr 3;216(4):961-981. (PMID: 28320825) Structure. 2010 May 12;18(5):616-26. (PMID: 20462495) Nat Cell Biol. 2010 Sep;12(9):894-901. (PMID: 20729837) Mol Biol Cell. 2004 Nov;15(11):5187-96. (PMID: 15371539) Curr Opin Struct Biol. 2007 Apr;17(2):221-8. (PMID: 17395454) Dev Cell. 2015 Dec 7;35(5):600-613. (PMID: 26651294) Nat Cell Biol. 2005 Dec;7(12):1248-55. (PMID: 16273096) Mol Biol Cell. 2010 Jun 15;21(12):1968-81. (PMID: 20427577) Genetics. 2013 Nov;195(3):1187-91. (PMID: 23979586) Biology (Basel). 2017 Jan 24;6(1):. (PMID: 28125021) Biology (Basel). 2017 Feb 17;6(1):. (PMID: 28218637) Nat Commun. 2015 Jun 02;6:7162. (PMID: 26031201) J Cell Biol. 2015 Sep 14;210(6):899-916. (PMID: 26347137) Nat Cell Biol. 2001 Nov;3(11):1001-7. (PMID: 11715021) Mol Cell. 2017 Nov 16;68(4):715-730.e5. (PMID: 29129638) Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772) Nature. 2017 Feb 23;542(7642):498-502. (PMID: 28102834) Trends Cell Biol. 2005 Jul;15(7):386-92. (PMID: 15922598) J Cell Biol. 2005 Apr 11;169(1):49-60. (PMID: 15824131) Open Biol. 2015 Nov;5(11):. (PMID: 26581576) Curr Biol. 2005 May 10;15(9):856-61. (PMID: 15886105) PLoS One. 2011;6(9):e24656. (PMID: 21935434) Mol Biol Cell. 2009 Feb;20(4):1252-67. (PMID: 19109417) Nat Genet. 2008 Nov;40(11):1375-83. (PMID: 18953339) Genes Dev. 2008 Sep 1;22(17):2385-99. (PMID: 18765790) PLoS One. 2011 Jan 28;6(1):e16494. (PMID: 21305043) Nat Cell Biol. 2015 Sep;17(9):1134-44. (PMID: 26258631) Cell Motil Cytoskeleton. 1996;35(2):121-33. (PMID: 8894282) Chromosoma. 1997 Dec;106(7):446-55. (PMID: 9391217) Science. 2013 Dec 6;342(6163):1239-42. (PMID: 24231804) Sci Rep. 2018 Mar 1;8(1):3888. (PMID: 29497093) J Cell Biol. 2014 Mar 3;204(5):647-57. (PMID: 24567362) FEBS Lett. 2014 Aug 25;588(17):3265-73. (PMID: 25064841) Curr Biol. 2015 Oct 19;25(20):R1002-18. (PMID: 26485365) Nat Commun. 2017 Jun 12;8:15822. (PMID: 28604727) AIMS Mol Sci. 2016;3(4):597-634. (PMID: 28920074) J Cell Biol. 1995 Aug;130(4):941-8. (PMID: 7642709) Curr Biol. 2005 Feb 8;15(3):214-25. (PMID: 15694304) Annu Rev Biochem. 2017 Jun 20;86:637-657. (PMID: 28471691) Curr Protoc Protein Sci. 2008 Feb;Chapter 5:Unit 5.20. (PMID: 18429060) Nat Methods. 2013 Oct;10(10):1028-34. (PMID: 23995389) J Cell Biol. 1998 Aug 10;142(3):763-74. (PMID: 9700164) Elife. 2015 Jan 22;4:e05269. (PMID: 25611342) J Cell Biol. 2001 Dec 24;155(7):1159-72. (PMID: 11756470) |
| فهرسة مساهمة: | Keywords: RZZ complex; dynein; fibrous corona; kinetochore; mitosis; spindle assembly checkpoint; spindly |
| المشرفين على المادة: | 0 (Cell Cycle Proteins) 0 (MAD1L1 protein, human) 0 (MAD2L1 protein, human) 0 (Mad2 Proteins) 0 (Nuclear Proteins) EC 3.6.4.2 (Dyneins) |
| تواريخ الأحداث: | Date Created: 20181113 Date Completed: 20191025 Latest Revision: 20231004 |
| رمز التحديث: | 20231004 |
| مُعرف محوري في PubMed: | PMC6224608 |
| DOI: | 10.1016/j.cub.2018.08.056 |
| PMID: | 30415699 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1879-0445 |
|---|---|
| DOI: | 10.1016/j.cub.2018.08.056 |