دورية أكاديمية
Effect of high-density lipoprotein on oocyte maturation and bovine embryo development in vitro.
| العنوان: | Effect of high-density lipoprotein on oocyte maturation and bovine embryo development in vitro. |
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| المؤلفون: | Rincón JAA; Universidade Federal de Pelotas, Pelotas, Brasil., Pradieé J; Universidade Federal de Pelotas, Pelotas, Brasil., Remião MH; Universidade Federal de Pelotas, Pelotas, Brasil., Collares TV; Universidade Federal de Pelotas, Pelotas, Brasil., Mion B; Universidade Federal de Pelotas, Pelotas, Brasil., Gasperin BG; Universidade Federal de Pelotas, Pelotas, Brasil., Tomazele Rovani M; Universidade Federal de Pelotas, Pelotas, Brasil., Corrêa MN; Universidade Federal de Pelotas, Pelotas, Brasil., Pegoraro LMC; Embrapa Clima Temperado, Pelotas, Brasil., Schneider A; Universidade Federal de Pelotas, Pelotas, Brasil. |
| المصدر: | Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2019 Mar; Vol. 54 (3), pp. 445-455. Date of Electronic Publication: 2018 Dec 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Paul Parey Scientific Publishers Country of Publication: Germany NLM ID: 9015668 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-0531 (Electronic) Linking ISSN: 09366768 NLM ISO Abbreviation: Reprod Domest Anim Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin ; Hamburg : Paul Parey Scientific Publishers, c1990- |
| مواضيع طبية MeSH: | Blastocyst/*physiology , Embryo Culture Techniques/*veterinary , In Vitro Oocyte Maturation Techniques/*veterinary , Lipoproteins, HDL/*pharmacology , Oocytes/*growth & development, Animals ; Apolipoprotein A-I/pharmacology ; Aryldialkylphosphatase/pharmacology ; Cattle ; Cumulus Cells/drug effects ; Female ; Fertilization in Vitro/veterinary ; Gene Expression ; Humans ; Oogenesis |
| مستخلص: | High-density lipoprotein (HDL) is the main lipoprotein in the follicular fluid, and it has anti-inflammatory, antioxidant and cryoprotectant properties. The anti-inflammatory potential and antioxidant potential are derived from its lipid composition, especially the apolipoprotein AI (ApoAI) and paraoxonase 1 (PON1). The aim of this study was to evaluate the effect of HDL during in vitro maturation (IVM) on oocyte maturation and early bovine embryo development. For this, cumulus-oocyte complexes (COCs) were obtained from bovine ovaries collected at a local slaughterhouse. COCs (n = 2,250) were allocated into three groups (n = 50 COCs/group) according to the addition of HDL protein (HDL-P) during IVM for 22 hr: 0 (control), 50 and 150 mg/dl. After IVM, COCs were inseminated (in vitro fertilization) and cultivated for 7 days. Total cholesterol concentration, total protein, triglycerides and ApoAI concentrations on IVM medium increased proportionally to HDL-P addition. However, PON1 activity was not detected in any treatment. The addition of HDL-P did not affect nuclear maturation rate, endogenous reactive oxygen species and glutathione levels in COCs (p > 0.05). The highest HDL-P concentration (150 mg/dl) decreased cleavage and blastocyst rate (p < 0.05). Moreover, the HDL-P 150 mg/dl group had lower cellular count/blastocyst than the 50 mg/dl group (p < 0.05). However, the addition of HDL-P did not affect relative gene expression of evaluated genes. In conclusion, the complex HDL/ApoAI obtained from human plasma, in the absence of PON1 activity during in vitro oocyte maturation, decreased initial embryo development. (© 2018 Blackwell Verlag GmbH.) |
| معلومات مُعتمدة: | Conselho Nacional de Desenvolvimento Científico e Tecnológico; Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Empresa Brasileira de Pesquisa Agropecuária |
| فهرسة مساهمة: | Keywords: HDL; antioxidant; paraoxonase |
| سلسلة جزيئية: | GENBANK NM_001205596.1; NR_036642.1; NM_173894.1; NM_001099092.1; XM_002688367.3; NM_174681.2; NM_001031752.1; NM_174602.2; NM_001025325.2; NM_001113302.1; NM_173959.4; NM_001166530.1; NM_001024693.1; NM_174597.2 |
| المشرفين على المادة: | 0 (Apolipoprotein A-I) 0 (Lipoproteins, HDL) EC 3.1.8.1 (Aryldialkylphosphatase) |
| تواريخ الأحداث: | Date Created: 20181113 Date Completed: 20190502 Latest Revision: 20190502 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1111/rda.13373 |
| PMID: | 30417448 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1439-0531 |
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| DOI: | 10.1111/rda.13373 |