دورية أكاديمية
Evaluation of Vitek MS for Differentiation of Cryptococcus neoformans and Cryptococcus gattii Genotypes.
| العنوان: | Evaluation of Vitek MS for Differentiation of Cryptococcus neoformans and Cryptococcus gattii Genotypes. |
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| المؤلفون: | Siqueira LPM; Laboratorio de Micologia Médica (LIM 53), Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil., Gimenes VMF; Laboratorio de Micologia Médica (LIM 53), Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil., de Freitas RS; Laboratorio de Micologia Médica (LIM 53), Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil., Melhem MSC; Divisão Técnica de Biologia Médica, Seção de Micologia, Instituto Adolfo Lutz, São Paulo, Brazil., Bonfietti LX; Divisão Técnica de Biologia Médica, Seção de Micologia, Instituto Adolfo Lutz, São Paulo, Brazil., da Silva AR Jr; Laboratório Central (LIM 03), Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Souza Santos LB; Laboratorio de Micologia Médica (LIM 53), Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil., Motta AL; Laboratório Central (LIM 03), Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Rossi F; Laboratório Central (LIM 03), Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Benard G; Laboratorio de Micologia Médica (LIM 53), Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil., de Almeida JN Jr; Laboratorio de Micologia Médica (LIM 53), Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil jnaj99@gmail.com.; Laboratório Central (LIM 03), Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil. |
| المصدر: | Journal of clinical microbiology [J Clin Microbiol] 2019 Jan 02; Vol. 57 (1). Date of Electronic Publication: 2019 Jan 02 (Print Publication: 2019). |
| نوع المنشور: | Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7505564 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1098-660X (Electronic) Linking ISSN: 00951137 NLM ISO Abbreviation: J Clin Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Cryptococcosis/*microbiology , Cryptococcus gattii/*genetics , Cryptococcus neoformans/*genetics , Mycological Typing Techniques/*methods , Mycological Typing Techniques/*standards , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*standards, Cryptococcus gattii/chemistry ; Cryptococcus gattii/classification ; Cryptococcus gattii/isolation & purification ; Cryptococcus neoformans/chemistry ; Cryptococcus neoformans/classification ; Cryptococcus neoformans/isolation & purification ; Databases, Genetic ; Genotype ; Humans |
| مستخلص: | Cryptococcus neoformans and Cryptococcus gattii are the main pathogenic species of invasive cryptococcosis among the Cryptococcus species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proposed as an alternative method for labor-intensive methods for C. neoformans and C. gattii genotype differentiation. However, Vitek MS, one of the commercial MALDI-TOF MS instruments, has not been yet been evaluated for this purpose. Thus, we constructed an in-house database with reference strains belonging to the different C. neoformans (VNI, VNII, VNIII, and VNIV) and C. gattii (VGI, VGII, VGIII, and VGIV) major molecular types by using the software Saramis Premium (bioMérieux, Marcy-l'Etoile, France). Then, this new database was evaluated for discrimination of the different genotypes. Our in-house database provided correct identification for all C. neoformans and C. gattii genotypes; however, due to the intergenotypic mass spectral similarities, a careful postanalytic evaluation is necessary to provide correct genotype identification. (Copyright © 2019 American Society for Microbiology.) |
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| فهرسة مساهمة: | Keywords: Cryptococcus gattii; Cryptococcus neoformans; MALDI-TOF; VITEK MS; genotypic identification |
| تواريخ الأحداث: | Date Created: 20181116 Date Completed: 20200130 Latest Revision: 20200309 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC6322467 |
| DOI: | 10.1128/JCM.01282-18 |
| PMID: | 30429250 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-660X |
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| DOI: | 10.1128/JCM.01282-18 |