دورية أكاديمية
أعرض في EDS

A combined banding method that allows the reliable identification of chromosomes as well as differentiation of AT- and GC-rich heterochromatin.

التفاصيل البيبلوغرافية
العنوان: A combined banding method that allows the reliable identification of chromosomes as well as differentiation of AT- and GC-rich heterochromatin.
المؤلفون: Lemskaya NA; Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia. lemnat@mcb.nsc.ru., Kulemzina AI; Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia., Beklemisheva VR; Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia., Biltueva LS; Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia., Proskuryakova AA; Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia.; Novosibirsk State University, Novosibirsk, Russia., Hallenbeck JM; Stroke Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health (NINDS/NIH), Bethesda, MD, USA., Perelman PL; Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia.; Novosibirsk State University, Novosibirsk, Russia., Graphodatsky AS; Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia.; Novosibirsk State University, Novosibirsk, Russia.
المصدر: Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology [Chromosome Res] 2018 Dec; Vol. 26 (4), pp. 307-315. Date of Electronic Publication: 2018 Nov 15.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 9313452 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-6849 (Electronic) Linking ISSN: 09673849 NLM ISO Abbreviation: Chromosome Res Subsets: MEDLINE
أسماء مطبوعة: Publication: Dordrecht : Kluwer Academic Publishers
Original Publication: Oxford, UK : Rapid Communications of Oxford, c1993-
مواضيع طبية MeSH: Chromosome Banding/*methods , Heterochromatin/*chemistry, Animals ; Base Composition ; Fluorescent Dyes ; Formamides/pharmacology ; Karyotyping ; Nucleic Acid Denaturation ; Staining and Labeling
مستخلص: Сonstitutive heterochromatin areas are revealed by differential staining as C-positive chromosomal regions. These C-positive bands may greatly vary by location, size, and nucleotide composition. CBG-banding is the most commonly used method to detect structural heterochromatin in animals. The difficulty in identification of individual chromosomes represents an unresolved problem of this method as the body of the chromosome is stained uniformly and does not have banding pattern beyond C-bands. Here, we present the method that we called CDAG for sequential heterochromatin staining after differential GTG-banding. The method uses G-banding followed by heat denaturation in the presence of formamide with consecutive fluorochrome staining. The new technique is valid for the concurrent revealing of heterochromatin position due to differential banding of chromosomes and heterochromatin composition (AT-/GC-rich) in animal karyotyping.
References: Hum Mol Genet. 2003 Dec 1;12(23):3109-21. (PMID: 14519686)
Lancet. 1971 Oct 30;2(7731):971-2. (PMID: 4107917)
Chromosome Res. 2004;12(4):317-35. (PMID: 15241012)
Genet Sel Evol. 1986;18(2):111-22. (PMID: 22879236)
J Med Genet. 1980 Feb;17(1):72-3. (PMID: 7365768)
Cytogenet Genome Res. 2016;148(1):25-34. (PMID: 27088853)
Chromosoma. 1990 Apr;99(1):3-10. (PMID: 2340757)
Hum Genet. 1977 May 10;36(3):299-305. (PMID: 67077)
Exp Cell Res. 1976 Mar 15;98(2):411-23. (PMID: 56279)
Mol Cytogenet. 2016 Mar 22;9:28. (PMID: 27011768)
Chromosoma. 1976 Nov 29;58(4):307-24. (PMID: 137107)
Hereditas. 2002;137(2):145-8. (PMID: 12627841)
Science. 1966 Jan 28;151(3709):417-25. (PMID: 5322971)
Hum Genet. 1978 Feb 23;41(1):1-9. (PMID: 75832)
Chromosoma. 1971;34(3):243-53. (PMID: 5112131)
Nucleic Acids Res. 2012 Dec;40(22):11178-88. (PMID: 23066104)
Cytogenetics. 1971;10(2):81-6. (PMID: 4106483)
J Mol Biol. 1973 Dec 15;81(3):273-84. (PMID: 4767457)
Chromosoma. 2009 Aug;118(4):419-35. (PMID: 19412619)
Exp Cell Res. 1976 Oct 15;102(2):408-13. (PMID: 61885)
Nature. 1961 Apr 22;190:372-3. (PMID: 13764598)
Biotech Histochem. 1994 Sep;69(5):243-8. (PMID: 7529568)
Hum Genet. 1977 Apr 15;36(2):167-72. (PMID: 67075)
Chromosome Res. 2004;12(8):767-76. (PMID: 15702415)
Cytogenet Genome Res. 2011;132(1-2):31-40. (PMID: 20720395)
Chromosome Res. 2002;10(2):117-26. (PMID: 11993932)
Cytogenet Cell Genet. 1988;47(4):192-6. (PMID: 3416653)
Hereditas. 1978;89(1):107-28. (PMID: 81195)
Genetics. 1937 Nov;22(6):641-9. (PMID: 17246869)
Cell. 1987 Sep 25;50(7):1101-8. (PMID: 2441880)
Exp Cell Res. 1970 Jan;59(1):69-75. (PMID: 4194430)
Exp Cell Res. 1959 Oct;18:415-8. (PMID: 14428474)
Chromosoma. 1993 May;102(5):325-32. (PMID: 8325164)
Clin Genet. 1972;3(5):388-95. (PMID: 4117330)
Exp Cell Res. 1972 Nov;75(1):304-6. (PMID: 4117921)
Exp Cell Res. 1971 Jan;64(1):41-8. (PMID: 5100411)
J Med Genet. 1976 Jun;13(3):235-6. (PMID: 58991)
معلومات مُعتمدة: 18-04-00826 International Russian Foundation for Basic Research; 16-14-10009 International Russian Science Foundation
فهرسة مساهمة: Keywords: AT-rich; C-banding; Chromosome; Differential staining; G-banding; GC-rich; Heterochromatin composition; Karyotype; Сonstitutive heterochromatin
المشرفين على المادة: 0 (Fluorescent Dyes)
0 (Formamides)
0 (Heterochromatin)
4781T907ZS (formamide)
تواريخ الأحداث: Date Created: 20181117 Date Completed: 20190710 Latest Revision: 20200309
رمز التحديث: 20221213
DOI: 10.1007/s10577-018-9589-9
PMID: 30443803
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-6849
DOI:10.1007/s10577-018-9589-9