دورية أكاديمية
[Application of GLAD-PCR analysis for the methylation sites detection in the regulatory areas of tumor-suppressor genes ELMo1 and EsR1 in colorectal cancer].
| العنوان: | [Application of GLAD-PCR analysis for the methylation sites detection in the regulatory areas of tumor-suppressor genes ELMo1 and EsR1 in colorectal cancer]. |
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| المؤلفون: | Evdokimov AA, Netesova NA, Smetannikova NA, Abdurashitov MA, Akishev AG, Davydovich ES, Ermolaev YD, Karpov AB, Sazonov AE, Takhauov RM, Degtyarev SK |
| المصدر: | Voprosy onkologii [Vopr Onkol] 2016; Vol. 62 (1), pp. 117-21. |
| نوع المنشور: | Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Nauchno-issledovatelʹskiĭ institut onkologii im. N.N. Petrova Country of Publication: Russia (Federation) NLM ID: 0413775 Publication Model: Print Cited Medium: Print ISSN: 0507-3758 (Print) Linking ISSN: 05073758 NLM ISO Abbreviation: Vopr Onkol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2013-: St Petersburg : Nauchno-issledovatelʹskiĭ institut onkologii im. N.N. Petrova Original Publication: St Petersburg : Izdatelstvo Meditsina, Leningradskoe Otdelenie |
| مواضيع طبية MeSH: | DNA Methylation* , Regulatory Sequences, Nucleic Acid*, Adaptor Proteins, Signal Transducing/*genetics , Colorectal Neoplasms/*genetics , DNA, Neoplasm/*genetics , Estrogen Receptor alpha/*genetics , Polymerase Chain Reaction/*methods , Tumor Suppressor Proteins/*genetics, Adaptor Proteins, Signal Transducing/metabolism ; Colorectal Neoplasms/metabolism ; Colorectal Neoplasms/pathology ; DNA, Neoplasm/metabolism ; Estrogen Receptor alpha/metabolism ; Female ; Humans ; Male ; Tumor Suppressor Proteins/metabolism |
| مستخلص: | Aberrant methylation of regulation regions of tumorsuppressor genes is showed for many cancer diseases. In course of this modification an enzyme DNMT3 methylates RCGY sites in CpG-islands of regulation regions producing R(5mC)GY sites. Earlier we developed GLAD-PCR assay to determine R(5mC)GY site in a definite position of human genome. In this work we have applied GLAD-PCR assay to determine R(5mC)GY sites in regulation regions of ESR1 and ELMO1 tumor-suppressor genes. We have studied a fragment of first exon of ELMO1 gene and a part of ESR1 promoter region in DNA preparations from malignant cell line SW837 and colorectal tumor samples. We have checked four sites in each region and found two highly methylated sites: GCGC in first exon of ELMO1 gene and GCGT in promoter region of ESR1 gene. Site GCGT is weakly methylated in healthy tissues and more methylated in the most of colorectal samples. Site GCGC is not methylated in healthy tissues and significantly methylated in 60% of colorectal samples. A possibility to use GLAD-PCR assay for cancer diagnostics is discussed. |
| المشرفين على المادة: | 0 (Adaptor Proteins, Signal Transducing) 0 (DNA, Neoplasm) 0 (ELMO1 protein, human) 0 (ESR1 protein, human) 0 (Estrogen Receptor alpha) 0 (Tumor Suppressor Proteins) |
| تواريخ الأحداث: | Date Created: 20181117 Date Completed: 20181211 Latest Revision: 20210816 |
| رمز التحديث: | 20221213 |
| PMID: | 30444589 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0507-3758 |
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