دورية أكاديمية
Chromatin Modifiers SET-25 and SET-32 Are Required for Establishment but Not Long-Term Maintenance of Transgenerational Epigenetic Inheritance.
| العنوان: | Chromatin Modifiers SET-25 and SET-32 Are Required for Establishment but Not Long-Term Maintenance of Transgenerational Epigenetic Inheritance. |
|---|---|
| المؤلفون: | Woodhouse RM; The University of Sydney, School of Life and Environmental Sciences, Sydney, NSW 2006, Australia., Buchmann G; The University of Sydney, School of Life and Environmental Sciences, Sydney, NSW 2006, Australia., Hoe M; The University of Sydney, School of Life and Environmental Sciences, Sydney, NSW 2006, Australia., Harney DJ; The University of Sydney, Charles Perkins Centre, School of Life and Environmental Sciences, Sydney, NSW 2006, Australia., Low JKK; The University of Sydney, School of Life and Environmental Sciences, Sydney, NSW 2006, Australia., Larance M; The University of Sydney, Charles Perkins Centre, School of Life and Environmental Sciences, Sydney, NSW 2006, Australia., Boag PR; Monash University, Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Clayton, VIC 3800, Australia., Ashe A; The University of Sydney, School of Life and Environmental Sciences, Sydney, NSW 2006, Australia; The University of Wollongong, School of Biological Sciences and Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia. Electronic address: alyson.ashe@sydney.edu.au. |
| المصدر: | Cell reports [Cell Rep] 2018 Nov 20; Vol. 25 (8), pp. 2259-2272.e5. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101573691 Publication Model: Print Cited Medium: Internet ISSN: 2211-1247 (Electronic) NLM ISO Abbreviation: Cell Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, c 2012- |
| مواضيع طبية MeSH: | Epigenesis, Genetic*, Caenorhabditis elegans/*genetics , Caenorhabditis elegans Proteins/*metabolism , Chromatin/*metabolism , Histone Methyltransferases/*metabolism , Histone-Lysine N-Methyltransferase/*metabolism , Inheritance Patterns/*genetics, Animals ; Caenorhabditis elegans/embryology ; Gene Silencing ; Germ Cells/metabolism ; Histones/metabolism ; Longevity ; Lysine/metabolism ; Male ; Methylation ; Models, Genetic ; Mutation/genetics ; Phenotype ; RNA/metabolism ; RNA, Small Interfering/metabolism ; Spermatozoa/metabolism |
| مستخلص: | Some epigenetic modifications are inherited from one generation to the next, providing a potential mechanism for the inheritance of environmentally acquired traits. Transgenerational inheritance of RNAi phenotypes in Caenorhabditis elegans provides an excellent model to study this phenomenon, and although studies have implicated both chromatin modifications and small RNA pathways in heritable silencing, their relative contributions remain unclear. Here, we demonstrate that the putative histone methyltransferases SET-25 and SET-32 are required for establishment of a transgenerational silencing signal but not for long-term maintenance of this signal between subsequent generations, suggesting that transgenerational epigenetic inheritance is a multi-step process with distinct genetic requirements for establishment and maintenance of heritable silencing. Furthermore, small RNA sequencing reveals that the abundance of secondary siRNAs (thought to be the effector molecules of heritable silencing) does not correlate with silencing phenotypes. Together, our results suggest that the current mechanistic models of epigenetic inheritance are incomplete. (Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.) |
| التعليقات: | Comment in: Epigenet Insights. 2019 Apr 15;12:2516865719844214. (PMID: 31020270) |
| معلومات مُعتمدة: | P40 OD010440 United States OD NIH HHS |
| فهرسة مساهمة: | Keywords: RNAi; chromatin; epigenetics; inheritance; small RNA; transgenerational |
| المشرفين على المادة: | 0 (Caenorhabditis elegans Proteins) 0 (Chromatin) 0 (Histones) 0 (RNA, Small Interfering) 63231-63-0 (RNA) EC 2.1.1.- (Histone Methyltransferases) EC 2.1.1.- (set-32 protein, C elegans) EC 2.1.1.43 (Histone-Lysine N-Methyltransferase) EC 2.1.1.43 (SET-25 protein, C elegans) K3Z4F929H6 (Lysine) |
| تواريخ الأحداث: | Date Created: 20181122 Date Completed: 20191211 Latest Revision: 20200124 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.celrep.2018.10.085 |
| PMID: | 30463020 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2211-1247 |
|---|---|
| DOI: | 10.1016/j.celrep.2018.10.085 |