دورية أكاديمية
Tempol modulates the leukocyte response to inflammatory stimuli and attenuates endotoxin-induced sickness behaviour in mice.
| العنوان: | Tempol modulates the leukocyte response to inflammatory stimuli and attenuates endotoxin-induced sickness behaviour in mice. |
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| المؤلفون: | Lima SNP; Department of Biochemistry (DBq), Institute of Biomedical Sciences, Federal University of Alfenas, Alfenas, Brazil., Cerdeira CD; Department of Biochemistry (DBq), Institute of Biomedical Sciences, Federal University of Alfenas, Alfenas, Brazil., Santos GB; Department of Biochemistry (DBq), Institute of Biomedical Sciences, Federal University of Alfenas, Alfenas, Brazil., Fernandes MM; Department of Biochemistry (DBq), Institute of Biomedical Sciences, Federal University of Alfenas, Alfenas, Brazil., Giusti-Paiva A; Department of Physiology, Institute of Biomedical Sciences; Federal University of Alfenas, Alfenas, Brazil., Brigagão MRPL; Department of Biochemistry (DBq), Institute of Biomedical Sciences, Federal University of Alfenas, Alfenas, Brazil. |
| المصدر: | Archives of physiology and biochemistry [Arch Physiol Biochem] 2020 Oct; Vol. 126 (4), pp. 341-347. Date of Electronic Publication: 2018 Nov 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Informa Healthcare Country of Publication: England NLM ID: 9510153 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1744-4160 (Electronic) Linking ISSN: 13813455 NLM ISO Abbreviation: Arch Physiol Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Informa Healthcare Original Publication: Lisse, Netherlands : Swets & Zeitlinger, c1995- |
| مواضيع طبية MeSH: | Behavior, Animal/*drug effects , Cyclic N-Oxides/*pharmacology , Endotoxins/*pharmacology , Leukocytes/*drug effects, Animals ; Cyclic N-Oxides/therapeutic use ; Dose-Response Relationship, Drug ; Inflammation/immunology ; Leukocytes/metabolism ; Locomotion/drug effects ; Male ; Mice ; Peritonitis/chemically induced ; Peritonitis/drug therapy ; Peritonitis/metabolism ; Spin Labels ; Superoxides/metabolism |
| مستخلص: | Background and aims : Lipopolysaccharide (LPS), an endotoxin, is a component of the outer membrane of Gram-negative bacteria that is able to activate the peripheral immune system, leading to changes in signalling pathways that act locally and systemically to achieve adaptive responses. Sickness behaviour is a motivational state in response to endotoxin exposure and includes depressed activity and a reduction of exploratory behaviour, potentially reorganising organism priorities to cope with infectious diseases. We hypothesised that 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (Tempol) modulates the leukocyte response to endotoxins and decreases LPS-induced sickness behaviour in mice. Methods : The effects of Tempol on LPS-induced peritonitis and the respiratory burst of neutrophils primed with LPS and triggered by phorbol 12-myristate-13-acetate (PMA) were evaluated. To evaluate the effects of Tempol on sickness behaviour, the mice were submitted to an open field and forced swim tests. Results : Tempol (50-100 μM/10 6 cells) decreased the respiratory burst of LPS-primed and PMA-stimulated neutrophils in vitro . In vivo , this nitroxide (30 and 100 mg/kg body weight) inhibited leukocyte migration to the peritoneal cavity after LPS administration in mice. Moreover, Tempol pretreatment (30 and 100 mg/kg body weight) before LPS administration also attenuated sickness behavioural changes. Conclusions : Together, these findings shed light on the mechanisms underlying the anti-inflammatory potential and confirm the therapeutic potential of nitroxides. |
| فهرسة مساهمة: | Keywords: Tempol; leucocytes; lipopolysaccharide; sickness behavioural; superoxide |
| المشرفين على المادة: | 0 (Cyclic N-Oxides) 0 (Endotoxins) 0 (Spin Labels) 11062-77-4 (Superoxides) U78ZX2F65X (tempol) |
| تواريخ الأحداث: | Date Created: 20181123 Date Completed: 20210503 Latest Revision: 20210503 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1080/13813455.2018.1538247 |
| PMID: | 30465447 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1744-4160 |
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| DOI: | 10.1080/13813455.2018.1538247 |