| المؤلفون: |
Kopylov AT; Institute of Biomedical Chemistry , Moscow 119435 , Russia., Ponomarenko EA; Institute of Biomedical Chemistry , Moscow 119435 , Russia., Ilgisonis EV; Institute of Biomedical Chemistry , Moscow 119435 , Russia., Pyatnitskiy MA; Institute of Biomedical Chemistry , Moscow 119435 , Russia., Lisitsa AV; Institute of Biomedical Chemistry , Moscow 119435 , Russia., Poverennaya EV; Institute of Biomedical Chemistry , Moscow 119435 , Russia., Kiseleva OI; Institute of Biomedical Chemistry , Moscow 119435 , Russia., Farafonova TE; Institute of Biomedical Chemistry , Moscow 119435 , Russia., Tikhonova OV; Institute of Biomedical Chemistry , Moscow 119435 , Russia., Zavialova MG; Institute of Biomedical Chemistry , Moscow 119435 , Russia., Novikova SE; Institute of Biomedical Chemistry , Moscow 119435 , Russia., Moshkovskii SA; Institute of Biomedical Chemistry , Moscow 119435 , Russia.; Pirogov Russian National Research Medical University , Moscow 117997 , Russia., Radko SP; Institute of Biomedical Chemistry , Moscow 119435 , Russia., Morukov BV; Institute of Medico-Biological Problems , Moscow 123007 , Russia., Grigoriev AI; Institute of Medico-Biological Problems , Moscow 123007 , Russia., Paik YK; Yonsei Proteome Research Center , Yonsei University , Seoul 03722 , Korea., Salekdeh GH; Department of Molecular Systems Biology, Cell Science Research Center , Royan Institute for Stem Cell Biology and Technology, ACECR , Tehran , Iran.; Department of Molecular Sciences , Macquarie University , Sydney , New South Wales 2109 , Australia.; Department of Systems Biology , Agricultural Biotechnology Research Institute of Iran , Karaj , Iran., Urbani A; Area of Diagnostic Laboratories , Fondazione Policlinico Gemelli-IRCCS , Rome 00168 , Italy.; Institute of Biochemistry and Clinical Biochemistry , Catholic University of the Sacred Heart , Rome 00168 , Italy., Zgoda VG; Institute of Biomedical Chemistry , Moscow 119435 , Russia., Archakov AI; Institute of Biomedical Chemistry , Moscow 119435 , Russia. |
| مستخلص: |
This work continues the series of the quantitative measurements of the proteins encoded by different chromosomes in the blood plasma of a healthy person. Selected Reaction Monitoring with Stable Isotope-labeled peptide Standards (SRM SIS) and a gene-centric approach, which is the basis for the implementation of the international Chromosome-centric Human Proteome Project (C-HPP), were applied for the quantitative measurement of proteins in human blood plasma. Analyses were carried out in the frame of C-HPP for each protein-coding gene of the four human chromosomes: 18, 13, Y, and mitochondrial. Concentrations of proteins encoded by 667 genes were measured in 54 blood plasma samples of the volunteers, whose health conditions were consistent with requirements for astronauts. The gene list included 276, 329, 47, and 15 genes of chromosomes 18, 13, Y, and the mitochondrial chromosome, respectively. This paper does not make claims about the detection of missing proteins. Only 205 proteins (30.7%) were detected in the samples. Of them, 84, 106, 10, and 5 belonged to chromosomes 18, 13, and Y and the mitochondrial chromosome, respectively. Each detected protein was found in at least one of the samples analyzed. The SRM SIS raw data are available in the ProteomeXchange repository (PXD004374, PASS01192). |
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