دورية أكاديمية
Dynamic Organellar Maps for Spatial Proteomics.
| العنوان: | Dynamic Organellar Maps for Spatial Proteomics. |
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| المؤلفون: | Itzhak DN; Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany., Schessner JP; Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany., Borner GHH; Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany. |
| المصدر: | Current protocols in cell biology [Curr Protoc Cell Biol] 2019 Jun; Vol. 83 (1), pp. e81. Date of Electronic Publication: 2018 Nov 29. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101287856 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1934-2616 (Electronic) Linking ISSN: 19342616 NLM ISO Abbreviation: Curr Protoc Cell Biol Subsets: MEDLINE |
| مواضيع طبية MeSH: | Organelles/*metabolism , Proteomics/*methods, Intracellular Space/metabolism ; Mass Spectrometry/methods ; Organelles/ultrastructure ; Proteins/analysis ; Proteins/metabolism ; Subcellular Fractions/chemistry |
| مستخلص: | Eukaryotic cells are highly compartmentalized and protein subcellular localization critically influences protein function. Identification of the subcellular localizations of proteins and their translocation events upon perturbation has mostly been confined to targeted studies or laborious microscopy-based methods. Here we describe a systematic mass spectrometry-based method for spatial proteomics. The approach uses simple fractionation profiling and has two applications: Firstly it can be used to infer subcellular protein localization on a proteome-wide scale, resulting in a protein map of the cell. Secondly, the method permits identification of changes in protein localization, by comparing maps made under different conditions, as a tool for unbiased systems cell biology. © 2018 by John Wiley & Sons, Inc. (© 2018 John Wiley & Sons, Inc.) |
| فهرسة مساهمة: | Keywords: mass spectrometry; organelle proteomics; protein dynamics; spatial proteomics; subcellular localization; systems biology |
| المشرفين على المادة: | 0 (Proteins) |
| تواريخ الأحداث: | Date Created: 20181130 Date Completed: 20200506 Latest Revision: 20200506 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1002/cpcb.81 |
| PMID: | 30489039 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1934-2616 |
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| DOI: | 10.1002/cpcb.81 |