دورية أكاديمية
أعرض في EDS

Abnormal brown adipose tissue mitochondrial structure and function in IL10 deficiency.

التفاصيل البيبلوغرافية
العنوان: Abnormal brown adipose tissue mitochondrial structure and function in IL10 deficiency.
المؤلفون: de-Lima-Júnior JC; Laboratory of Cell Signaling, Department of Internal Medicine, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil., Souza GF; Laboratory of Cell Signaling, Department of Internal Medicine, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil., Moura-Assis A; Laboratory of Cell Signaling, Department of Internal Medicine, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil., Gaspar RS; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil; CEPECE - Research Center of Sport Sciences, School of Applied Sciences, University of Campinas, Limeira, SP, Brazil(.)., Gaspar JM; Laboratory of Cell Signaling, Department of Internal Medicine, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil., Rocha AL; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP 13083-970, Brazil., Ferrucci DL; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, Campinas, São Paulo, Brazil; National Institute of Photonics Applied to Cell Biology (INFABiC), Campinas, São Paulo, Brazil., Lima TI; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP 13083-970, Brazil., Victório SC; Laboratory of Cell Signaling, Department of Internal Medicine, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil., Bonfante ILP; Laboratory of Exercise Physiology, School of Physical Education, University of Campinas, Campinas, SP 13083-970, Brazil., Cavaglieri CR; Laboratory of Exercise Physiology, School of Physical Education, University of Campinas, Campinas, SP 13083-970, Brazil., Pareja JC; Laboratory of Investigation in Metabolism and Diabetes (LIMED)/Gastrocentro, Department of Surgery, University of Campinas (UNICAMP), Campinas, SP 13081-970, Brazil., Brunetto SQ; Biomedical Engineering Center, University of Campinas (UNICAMP), Campinas, SP, Brazil., Ramos CD; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Department of Radiology, University of Campinas, Campinas, São Paulo 13084-970, Brazil., Geloneze B; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Laboratory of Investigation in Metabolism and Diabetes (LIMED)/Gastrocentro, Department of Surgery, University of Campinas (UNICAMP), Campinas, SP 13081-970, Brazil., Mori MA; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP 13083-970, Brazil., Silveira LR; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP 13083-970, Brazil., Segundo GRS; Department of Pediatrics, Federal University of Uberlandia, Uberlandia, Brazil., Ropelle ER; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil; CEPECE - Research Center of Sport Sciences, School of Applied Sciences, University of Campinas, Limeira, SP, Brazil(.)., Velloso LA; Laboratory of Cell Signaling, Department of Internal Medicine, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil. Electronic address: lavelloso@fcm.unicamp.br.
المصدر: EBioMedicine [EBioMedicine] 2019 Jan; Vol. 39, pp. 436-447. Date of Electronic Publication: 2018 Nov 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101647039 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2352-3964 (Electronic) Linking ISSN: 23523964 NLM ISO Abbreviation: EBioMedicine Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Elsevier B.V., [2014]-
مواضيع طبية MeSH: Adipose Tissue, Brown/*cytology , Inflammation/*pathology , Interleukin-10/*genetics , Mitochondria/*pathology , Shivering/*genetics, Adipose Tissue, Brown/metabolism ; Adipose Tissue, Brown/pathology ; Animals ; Caspases/genetics ; Cell Line ; Cold Temperature ; Computational Biology/methods ; Energy Metabolism ; Gene Knockout Techniques ; Humans ; Inflammation/genetics ; Inflammation/metabolism ; Lipid Metabolism ; Male ; Mice ; Mitochondria/genetics ; Mitochondria/metabolism ; Uncoupling Protein 1/metabolism
مستخلص: Background: Inflammation is the most relevant mechanism linking obesity with insulin-resistance and metabolic disease. It impacts the structure and function of tissues and organs involved in metabolism, such as the liver, pancreatic islets and the hypothalamus. Brown adipose tissue has emerged as an important component of whole body energy homeostasis, controlling caloric expenditure through the regulation of non-shivering thermogenesis. However, little is known about the impact of systemic inflammation on the structure and function of brown adipose tissue.
Methods: The relations between IL10 and mitochondria structure/function and also with thermogenesis were evaluated by bioinformatics using human and rodent data. Real-time PCR, immunoblot, fluorescence and transmission electron microscopy were employed to determine the effect of IL10 in the brown adipose tissue of wild type and IL10 knockout mice.
Findings: IL10 knockout mice, a model of systemic inflammation, present severe structural abnormalities of brown adipose tissue mitochondria, which are round-shaped with loss of cristae structure and increased fragmentation. IL10 deficiency leads to newborn cold intolerance and impaired UCP1-dependent brown adipose tissue mitochondrial respiration. The reduction of systemic inflammation with an anti-TNFα monoclonal antibody partially rescued the structural but not the functional abnormalities of brown adipose tissue mitochondria. Using bioinformatics analyses we show that in both humans and mice, IL10 transcripts correlate with mitochondrial lipid metabolism and caspase gene expression.
Interpretation: IL10 and systemic inflammation play a central role in the regulation of brown adipose tissue by controlling mitochondrial structure and function. FUND: Sao Paulo Research Foundation grant 2013/07607-8.
(Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.)
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فهرسة مساهمة: Keywords: Inflammation; Interleukin-10; Mitochondria; Obesity; Respiration; Thermogenesis
المشرفين على المادة: 0 (IL10 protein, human)
0 (UCP1 protein, human)
0 (Uncoupling Protein 1)
130068-27-8 (Interleukin-10)
EC 3.4.22.- (Caspases)
تواريخ الأحداث: Date Created: 20181204 Date Completed: 20190610 Latest Revision: 20200225
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6355943
DOI: 10.1016/j.ebiom.2018.11.041
PMID: 30502051
قاعدة البيانات: MEDLINE
الوصف
تدمد:2352-3964
DOI:10.1016/j.ebiom.2018.11.041