دورية أكاديمية
DNA damage in BV-2 cells: An important supplement to the neurotoxicity of CdTe quantum dots.
| العنوان: | DNA damage in BV-2 cells: An important supplement to the neurotoxicity of CdTe quantum dots. |
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| المؤلفون: | He K; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education; School of Public Health, & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing, 210009, China.; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, 210009, China., Liang X; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education; School of Public Health, & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing, 210009, China.; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, 210009, China., Wei T; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education; School of Public Health, & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing, 210009, China.; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, 210009, China., Liu N; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education; School of Public Health, & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing, 210009, China.; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, 210009, China., Wang Y; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education; School of Public Health, & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing, 210009, China.; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, 210009, China., Zou L; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education; School of Public Health, & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing, 210009, China.; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, 210009, China., Lu J; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education; School of Public Health, & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing, 210009, China.; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, 210009, China., Yao Y; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education; School of Public Health, & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing, 210009, China.; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, 210009, China., Kong L; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education; School of Public Health, & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing, 210009, China.; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, 210009, China., Zhang T; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education; School of Public Health, & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing, 210009, China.; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, 210009, China., Xue Y; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education; School of Public Health, & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing, 210009, China.; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, 210009, China., Wu T; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education; School of Public Health, & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing, 210009, China.; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, 210009, China., Tang M; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education; School of Public Health, & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing, 210009, China.; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, 210009, China. |
| المصدر: | Journal of applied toxicology : JAT [J Appl Toxicol] 2019 Mar; Vol. 39 (3), pp. 525-539. Date of Electronic Publication: 2018 Dec 04. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley And Sons Country of Publication: England NLM ID: 8109495 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1099-1263 (Electronic) Linking ISSN: 0260437X NLM ISO Abbreviation: J Appl Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Original Publication: [Philadelphia, Pa. : Heyden & Son, c1981- |
| مواضيع طبية MeSH: | DNA Damage*, Cadmium Compounds/*toxicity , Microglia/*drug effects , Neurotoxicity Syndromes/*etiology , Quantum Dots/*toxicity , Tellurium/*toxicity, Animals ; Cells, Cultured ; Glutathione/metabolism ; Mice ; Microglia/metabolism ; Microglia/ultrastructure ; Quantum Dots/metabolism ; Reactive Oxygen Species/metabolism ; Spectrophotometry, Atomic |
| مستخلص: | Microglial cells are resident immune cells in the central nervous system. Activation of microglia as induced by CdTe quantum dots (QDs) can trigger damage to neurons. To quantify the intracellular QDs, we monitored the intracellular Cd concentration in the QD-exposed mouse microglial cells (BV-2 cell line). The extent of cell injury at different times correlated with the Cd concentration in cells at that time. In addition to Cd ion detection, we also monitored the intracellular fluorescence of the QDs. More QDs accumulated in the nucleus than in the cytoplasm. Comet assays confirmed that QDs induce DNA damage. However, DNA cannot interact with QDs, so the DNA damage was not caused by CdTe QDs adducts to DNA but by the increase of the Cd ion concentration and the secondary oxidative damage. In addition to DNA damage, biofilm injury and endogenous reduced glutathione depletion were also apparent in QD-exposed BV-2 cells. These changes can be prevented or even reversed by exogenous reduced glutathione administration. (© 2018 John Wiley & Sons, Ltd.) |
| فهرسة مساهمة: | Keywords: BV-2 cells; DNA damage; ROS; glutathione; in vitro study; microglia; neurotoxicology; quantum dots |
| المشرفين على المادة: | 0 (Cadmium Compounds) 0 (Reactive Oxygen Species) GAN16C9B8O (Glutathione) NQA0O090ZJ (Tellurium) STG188WO13 (cadmium telluride) |
| تواريخ الأحداث: | Date Created: 20181205 Date Completed: 20200720 Latest Revision: 20200720 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1002/jat.3745 |
| PMID: | 30511393 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1099-1263 |
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| DOI: | 10.1002/jat.3745 |