دورية أكاديمية
أعرض في EDS

Enzyme-free synthesis of cyclic single-stranded DNA constructs containing a single triazole, amide or phosphoramidate backbone linkage and their use as templates for rolling circle amplification and nanoflower formation.

التفاصيل البيبلوغرافية
العنوان: Enzyme-free synthesis of cyclic single-stranded DNA constructs containing a single triazole, amide or phosphoramidate backbone linkage and their use as templates for rolling circle amplification and nanoflower formation.
المؤلفون: Chen J; Chemistry Research Laboratory , University of Oxford , Oxford , OX1 3TA , UK . Email: tom.brown@chem.ox.ac.uk., Baker YR; Chemistry Research Laboratory , University of Oxford , Oxford , OX1 3TA , UK . Email: tom.brown@chem.ox.ac.uk., Brown A; ATDBio , Magdalen Centre , Oxford Science Park , Oxford , OX4 4GA , UK., El-Sagheer AH; Chemistry Research Laboratory , University of Oxford , Oxford , OX1 3TA , UK . Email: tom.brown@chem.ox.ac.uk.; Chemistry Branch , Department of Science and Mathematics , Suez University , Suez 43721 , Egypt., Brown T; Chemistry Research Laboratory , University of Oxford , Oxford , OX1 3TA , UK . Email: tom.brown@chem.ox.ac.uk.
المصدر: Chemical science [Chem Sci] 2018 Aug 24; Vol. 9 (42), pp. 8110-8120. Date of Electronic Publication: 2018 Aug 24 (Print Publication: 2018).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101545951 Publication Model: eCollection Cited Medium: Print ISSN: 2041-6520 (Print) Linking ISSN: 20416520 NLM ISO Abbreviation: Chem Sci Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Cambridge, UK : Royal Society of Chemistry, [2010]-
مستخلص: Cyclic oligonucleotides are valuable targets with a broad range of potential applications spanning molecular biology and nanotechnology. Of particular importance is their role as templates in the rolling circle amplification (RCA) reaction. We describe three different chemical cyclisation methods for the preparation of single-stranded cyclic DNA constructs. These chemical cyclisation reactions are cheaper to carry out than the enzymatic reaction, and more amenable to preparative scale purification and characterisation of the cyclic product. They can also be performed under denaturing conditions and are therefore particularly valuable for cyclic DNA templates that contain secondary structures. The resulting single-stranded cyclic DNA constructs contain a single non-canonical backbone linkage at the ligation point (triazole, amide or phosphoramidate). They were compared to unmodified cyclic DNA in rolling circle amplification reactions using φ-29 and Bst 2.0 DNA polymerase enzymes. The cyclic templates containing a phosphoramidate linkage were particularly well tolerated by φ-29 polymerase, consistently performing as well in RCA as the unmodified DNA controls. Moreover, these phosphoramidate-modified cyclic constructs can be readily produced in oligonucleotide synthesis facilities from commercially available precursors. Phosphoramidate ligation therefore holds promise as a practical, scalable method for the synthesis of fully biocompatible cyclic RCA templates. The triazole-modified cyclic templates generally gave lower and more variable yields of RCA products, a significant proportion of which were double-stranded, while the performances of the templates containing an amide linkage lie in between those of the phosphoramidate- and triazole-containing templates.
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تواريخ الأحداث: Date Created: 20181214 Latest Revision: 20240403
رمز التحديث: 20240403
مُعرف محوري في PubMed: PMC6238721
DOI: 10.1039/c8sc02952k
PMID: 30542561
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-6520
DOI:10.1039/c8sc02952k