دورية أكاديمية
Influence of glucose transporter 1 activity inhibition on neuroblastoma in vitro.
| العنوان: | Influence of glucose transporter 1 activity inhibition on neuroblastoma in vitro. |
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| المؤلفون: | Peng Y; Department of Physiology, Chongqing Medical University, Chongqing 400016, China; Department of Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China., Xing SN; Department of Physiology, Chongqing Medical University, Chongqing 400016, China; Department of Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China., Tang HY; Department of Physiology, Chongqing Medical University, Chongqing 400016, China; Department of Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China., Wang CD; Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China; Department of Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China., Yi FP; Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China; Department of Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China., Liu GL; Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China; Department of Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China., Wu XM; Department of Physiology, Chongqing Medical University, Chongqing 400016, China; Department of Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China. Electronic address: xmwu@cqmu.edu.cn. |
| المصدر: | Gene [Gene] 2019 Mar 20; Vol. 689, pp. 11-17. Date of Electronic Publication: 2018 Dec 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland, 1976- |
| مواضيع طبية MeSH: | Glucose Transporter Type 1/*antagonists & inhibitors , Hydroxybenzoates/*pharmacology , Neuroblastoma/*metabolism , Neuroblastoma/*pathology, Apoptosis/drug effects ; Cell Cycle Checkpoints/drug effects ; Cell Cycle Checkpoints/genetics ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Cell Proliferation/genetics ; Cell Survival/drug effects ; Cell Survival/genetics ; Gene Expression Regulation, Neoplastic/drug effects ; Glucose Transporter Type 1/genetics ; Glycolysis/drug effects ; Glycolysis/genetics ; Humans ; Neuroblastoma/genetics |
| مستخلص: | Most cancer cells predominantly produce their energy through a high rate of glycolysis in the presence of abundant oxygen. Glycolysis has become a target of anticancer strategies. Previous researches showed that glucose transporter 1 (GLUT1) inhibitor is effective as anticancer agents. This study assessed the effects of the selective GLUT1 inhibitor WZB117 on regulation of neuroblastoma (NB) cell line SH-SY5Y viability, cell cycle and glycolysis in vitro. SH-SY5Y cells were grown and treated with WZB117 for up to 72 h and then subjected to cell viability, qRT-PCR, Western blot and flow cytometry analysis. Level of ATP and LDH was also analyzed. The result showed that WZB117 treatment reduced tumor cells viability, downregulated level of GLUT1 protein. Moreover, WZB117 treatment arrested tumor cells at the G0-G1 phase of the cell cycle, induced tumor cells to undergo necrosis instead of apoptosis. In addition, WZB117 treatment downregulated the levels of intracellular ATP, LDH and glycolytic enzymes. Thus, WZB117-induced GLUT1 inhibition suppressed tumor cell growth, induced cell cycle arrest and reduced glycolysis metabolites in NB cells in vitro. This study suggested that GLUT1 can be used as a potential therapeutic target for NB. (Copyright © 2018 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Energy metabolism; GLUT1; Glycolysis; Neuroblastoma |
| المشرفين على المادة: | 0 (Glucose Transporter Type 1) 0 (Hydroxybenzoates) 0 (SLC2A1 protein, human) 0 (WZB117) |
| تواريخ الأحداث: | Date Created: 20181217 Date Completed: 20190207 Latest Revision: 20190215 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.gene.2018.12.010 |
| PMID: | 30553996 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0038 |
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| DOI: | 10.1016/j.gene.2018.12.010 |