دورية أكاديمية
أعرض في EDS

Identification, Molecular Characterization, and Biology of a Novel Quadrivirus Infecting the Phytopathogenic Fungus Leptosphaeria biglobosa .

التفاصيل البيبلوغرافية
العنوان: Identification, Molecular Characterization, and Biology of a Novel Quadrivirus Infecting the Phytopathogenic Fungus Leptosphaeria biglobosa .
المؤلفون: Shah UA; Department of Biological and Environmental Sciences, University of Hertfordshire, Hatfield AL10 9AB, UK. unnatishah009@gmail.com., Kotta-Loizou I; Department of Biological and Environmental Sciences, University of Hertfordshire, Hatfield AL10 9AB, UK. i.kotta-loizou@imperial.ac.uk.; Department of Life Sciences, Imperial College London, London SW7 2AZ, UK. i.kotta-loizou@imperial.ac.uk., Fitt BDL; Department of Biological and Environmental Sciences, University of Hertfordshire, Hatfield AL10 9AB, UK. b.fitt@herts.ac.uk., Coutts RHA; Department of Biological and Environmental Sciences, University of Hertfordshire, Hatfield AL10 9AB, UK. r.coutts@herts.ac.uk.
المصدر: Viruses [Viruses] 2018 Dec 25; Vol. 11 (1). Date of Electronic Publication: 2018 Dec 25.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Genome, Viral* , Phylogeny*, Ascomycota/*virology , Fungal Viruses/*genetics, Ascomycota/growth & development ; Fungal Viruses/isolation & purification ; Plant Diseases/microbiology ; RNA Viruses/genetics ; RNA Viruses/isolation & purification ; RNA, Double-Stranded ; RNA, Viral/genetics ; Sequence Analysis, DNA ; Viral Structural Proteins/genetics ; Virulence
مستخلص: Here we report the molecular characterisation of a novel dsRNA virus isolated from the filamentous, plant pathogenic fungus Leptosphaeria biglobosa and known to cause significant alterations to fungal pigmentation and growth and to result in hypervirulence, as illustrated by comparisons between virus-infected and -cured isogenic fungal strains. The virus forms isometric particles approximately 40⁻45 nm in diameter and has a quadripartite dsRNA genome structure with size ranges of 4.9 to 4 kbp, each possessing a single ORF. Sequence analysis of the putative proteins encoded by dsRNAs 1⁻4, termed P1⁻P4, respectively, revealed modest similarities to the amino acid sequences of equivalent proteins predicted from the nucleotide sequences of known and suspected members of the family Quadriviridae and for that reason the virus was nominated Leptosphaeria biglobosa quadrivirus-1 (LbQV-1). Sequence and phylogenetic analysis using the P3 sequence, which encodes an RdRP, revealed that LbQV-1 was most closely related to known and suspected quadriviruses and monopartite totiviruses rather than other quadripartite mycoviruses including chrysoviruses and alternaviruses. Of the remaining encoded proteins, LbQV-1 P2 and P4 are structural proteins but the function of P1 is unknown. We propose that LbQV-1 is a novel member of the family Quadriviridae .
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معلومات مُعتمدة: BB/N005112/1 United Kingdom Biotechnology and Biological Sciences Research Council; BB/M028348/1 United Kingdom Biotechnology and Biological Sciences Research Council; BBP00489X/1 United Kingdom Biotechnology and Biological Sciences Research Council; BB/I017585/2 United Kingdom Biotechnology and Biological Sciences Research Council
فهرسة مساهمة: Keywords: Leptosphaeria biglobosa quadrivirus; dsRNA mycoviruses; fungal viruses; hypervirulence
المشرفين على المادة: 0 (RNA, Double-Stranded)
0 (RNA, Viral)
0 (Viral Structural Proteins)
تواريخ الأحداث: Date Created: 20181227 Date Completed: 20190513 Latest Revision: 20231005
رمز التحديث: 20231005
مُعرف محوري في PubMed: PMC6356713
DOI: 10.3390/v11010009
PMID: 30585188
قاعدة البيانات: MEDLINE
الوصف
تدمد:1999-4915
DOI:10.3390/v11010009