دورية أكاديمية
Quercetin sensitizes human myeloid leukemia KG-1 cells against TRAIL-induced apoptosis.
| العنوان: | Quercetin sensitizes human myeloid leukemia KG-1 cells against TRAIL-induced apoptosis. |
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| المؤلفون: | Naimi A; Department of Immunology, Division of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Entezari A; Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran., Hagh MF; Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Hassanzadeh A; Department of Immunology, Division of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Saraei R; Department of Immunology, Division of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Solali S; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.; Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. |
| المصدر: | Journal of cellular physiology [J Cell Physiol] 2019 Aug; Vol. 234 (8), pp. 13233-13241. Date of Electronic Publication: 2018 Dec 27. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 0050222 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4652 (Electronic) Linking ISSN: 00219541 NLM ISO Abbreviation: J Cell Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Wiley-Liss Original Publication: Philadelphia, Wistar Institute of Anatomy and Biology. |
| مواضيع طبية MeSH: | Antineoplastic Combined Chemotherapy Protocols/*pharmacology , Apoptosis/*drug effects , Leukemia, Myeloid, Acute/*pathology , Quercetin/*pharmacology , TNF-Related Apoptosis-Inducing Ligand/*pharmacology, Cell Line, Tumor ; Drug Synergism ; Humans |
| مستخلص: | Introduction: Acute myeloid leukemia (AML) is the most common acute leukemia in adults. Conventional treatments are associated with cytotoxicity and systemic side effects. Hence, efforts in the field of cancer treatment are focused on finding the strategies which can specifically target the tumor cells without affecting the normal cells. TNF-related apoptosis-inducing ligand (TRAIL) is a biological cytokine which has the mentioned specificity, but the resistance of some cancer cells limits its use as a therapeutic strategy. Recent studies have shown that quercetin (QUR) can be used as a sensitizing agent alongside with TRAIL. The present study showed that QUR can increase the effect of TRAIL-induced cytotoxicity in KG-1 cells. Materials and Methods: In this descriptive study, the IC50 dose for QUR in the KG-1 cell line was first determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay. Then, the cells were treated with TRAIL and QUR for 12, 24, and 48 hr. The rate of apoptosis was measured by Annexin V/propidium iodide assay. Also, the molecular evaluation of candidate genes was accomplished before and after the treatment. Results: The results indicated that QUR could sensitize the KG-1 cells against the TRAIL-induced apoptosis. This outcome is achieved by increasing the messenger RNA expression levels of the death receptor genes and reducing the expression of antiapoptotic proteins, as well as decreasing the expression of the NF-κB subunit. Conclusion: Our findings suggest that QUR can sensitize the acute myeloid KG-1 cells against TRAIL. Moreover, the combinational therapy of these agents might promisingly improve the clinical efficacy of TRAIL in patients with AML. (© 2018 Wiley Periodicals, Inc.) |
| فهرسة مساهمة: | Keywords: TNF-related apoptosis-inducing ligand (TRAIL); acute myeloid leukemia (AML); apoptosis; leukemia; quercetin (QUR) |
| المشرفين على المادة: | 0 (TNF-Related Apoptosis-Inducing Ligand) 0 (TNFSF10 protein, human) 9IKM0I5T1E (Quercetin) |
| تواريخ الأحداث: | Date Created: 20181228 Date Completed: 20200508 Latest Revision: 20200508 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1002/jcp.27995 |
| PMID: | 30589076 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1097-4652 |
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| DOI: | 10.1002/jcp.27995 |