دورية أكاديمية
Multidrug Resistance and the Predominance of blaCTX-M in Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae of Animal and Water Origin.
| العنوان: | Multidrug Resistance and the Predominance of blaCTX-M in Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae of Animal and Water Origin. |
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| المؤلفون: | Hassen B; Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, Tunis, Tunisia., Sghaier S; Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, Tunis, Tunisia., Abbassi MS; Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, Tunis, Tunisia, salahtoumi_mohamed@yahoo.com., Ferjani MA; Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, Tunis, Tunisia., Ben Said M; Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, Tunis, Tunisia., Hassen A; Laboratoire de Traitement des Eaux Usées, Centre des Recherches et des Technologies des Eaux (CERTE), Technopole Borj-Cédria, Soliman, Tunisia., Hammami S; Université La Manouba, École Nationale de Médecine Vétérinaire de Sidi Thabet, Sidi Thabet, Tunisia. |
| المصدر: | Journal of molecular microbiology and biotechnology [J Mol Microbiol Biotechnol] 2018; Vol. 28 (4), pp. 201-206. Date of Electronic Publication: 2019 Jan 04. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Karger Country of Publication: Switzerland NLM ID: 100892561 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1660-2412 (Electronic) Linking ISSN: 14641801 NLM ISO Abbreviation: J Mol Microbiol Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2003- : Basel ; New York : Karger Original Publication: Wymonham, Norfolk, UK : Horizon Scientific Press, c1999- |
| مواضيع طبية MeSH: | Anti-Bacterial Agents/*pharmacology , Drug Resistance, Multiple, Bacterial/*genetics , Enterobacteriaceae/*genetics , beta-Lactamases/*genetics, Animals ; Cefotaxime/pharmacology ; Drug Resistance, Multiple, Bacterial/drug effects ; Enterobacteriaceae/classification ; Enterobacteriaceae/drug effects ; Enterobacteriaceae/isolation & purification ; Microbial Sensitivity Tests ; Polymerase Chain Reaction ; Rivers/microbiology ; Tunisia ; Wastewater/microbiology ; Water Purification ; beta-Lactamases/metabolism |
| مستخلص: | The aim of this work was the genetic characterization of cefotaxime-resistant enterobacteria from animals (53 samples), the surface water of rivers (17 samples), and wastewater treatment plants (43 samples) in Tunisia. A total of 48 (42.4%) cefotaxime-resistant isolates were recovered. An extended spectrum beta-lactamase (ESBL) phenotype with a positive double-disk synergy test (DDST) was exhibited by 34 (70.8%) and 14 (29.1%) isolates from water and animal origins, respectively. Isolates from water were identified as: Escherichia coli (n = 17), Hafnia spp. (n = 13), Citrobacter spp. (n = 1), Enterobacter cloacae (n = 1), Klebsiella pneumoniae (n = 1), and K. oxytoca (n = 1). Animal isolates were identified as: E. coli (n = 11), E. cloacae (n = 1), Hafnia spp. (n = 1), and K. pneumoniae (n = 1). PCR investigation of blaCTX-M, blaTEM, and blaSHV genes showed that amongst the 48 isolates with a positive DDST, 41 (87.5%) carried the blaCTX-M gene, 1 isolate harbored the blaSHV gene, and 1 isolate coharbored blaCTX-M with blaSHV genes. The class 1 and 2 integrons were detected in 27 (56.2%) and 1 (2%) isolates, respectively. Our study showed a significant occurrence of ESBL-producing enterobacteria in animals and aquatic environments with a predominance of blaCTX-M genes. (© 2019 S. Karger AG, Basel.) |
| فهرسة مساهمة: | Keywords: Animal; Enterobacteriaceae; Extended spectrum beta-lactamase; Integrons; Multidrug resistance; Water |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Waste Water) EC 3.5.2.6 (beta-Lactamases) N2GI8B1GK7 (Cefotaxime) |
| تواريخ الأحداث: | Date Created: 20190107 Date Completed: 20190715 Latest Revision: 20221207 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1159/000495409 |
| PMID: | 30612122 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1660-2412 |
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| DOI: | 10.1159/000495409 |