دورية أكاديمية
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Chemically induced vesiculation as a platform for studying TMEM16F activity.

التفاصيل البيبلوغرافية
العنوان: Chemically induced vesiculation as a platform for studying TMEM16F activity.
المؤلفون: Han TW; Howard Hughes Medical Institute, University of California, San Francisco, CA 94143.; Department of Physiology, University of California, San Francisco, CA 94143.; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143., Ye W; Howard Hughes Medical Institute, University of California, San Francisco, CA 94143.; Department of Physiology, University of California, San Francisco, CA 94143.; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143., Bethel NP; Department of Pharmaceutical Chemistry, Cardiovascular Research Institute, University of California, San Francisco, CA 94143., Zubia M; Howard Hughes Medical Institute, University of California, San Francisco, CA 94143.; Department of Physiology, University of California, San Francisco, CA 94143.; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143., Kim A; Howard Hughes Medical Institute, University of California, San Francisco, CA 94143.; Department of Physiology, University of California, San Francisco, CA 94143.; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143., Li KH; Mass Spectrometry Facility, University of California, San Francisco, CA 94143., Burlingame AL; Mass Spectrometry Facility, University of California, San Francisco, CA 94143., Grabe M; Department of Pharmaceutical Chemistry, Cardiovascular Research Institute, University of California, San Francisco, CA 94143., Jan YN; Howard Hughes Medical Institute, University of California, San Francisco, CA 94143.; Department of Physiology, University of California, San Francisco, CA 94143.; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143., Jan LY; Howard Hughes Medical Institute, University of California, San Francisco, CA 94143; Lily.Jan@ucsf.edu.; Department of Physiology, University of California, San Francisco, CA 94143.; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143.
المصدر: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2019 Jan 22; Vol. 116 (4), pp. 1309-1318. Date of Electronic Publication: 2019 Jan 08.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : National Academy of Sciences
مواضيع طبية MeSH: Anoctamins/*metabolism , Biological Transport/*physiology , Phospholipid Transfer Proteins/*metabolism, Animals ; Calcium/metabolism ; Cell Line ; Cell Line, Tumor ; Cell Membrane/metabolism ; Cell-Derived Microparticles/metabolism ; Extracellular Vesicles/metabolism ; HEK293 Cells ; Humans ; Mice ; Phospholipids/metabolism
مستخلص: Calcium-activated phospholipid scramblase mediates the energy-independent bidirectional translocation of lipids across the bilayer, leading to transient or, in the case of apoptotic scrambling, sustained collapse of membrane asymmetry. Cells lacking TMEM16F-dependent lipid scrambling activity are deficient in generation of extracellular vesicles (EVs) that shed from the plasma membrane in a Ca 2+ -dependent manner, namely microvesicles. We have adapted chemical induction of giant plasma membrane vesicles (GPMVs), which require both TMEM16F-dependent phospholipid scrambling and calcium influx, as a kinetic assay to investigate the mechanism of TMEM16F activity. Using the GPMV assay, we identify and characterize both inactivating and activating mutants that elucidate the mechanism for TMEM16F activation and facilitate further investigation of TMEM16F-mediated lipid translocation and its role in extracellular vesiculation.
Competing Interests: The authors declare no conflict of interest.
References: Nature. 2014 Mar 6;507(7490):118-23. (PMID: 24487619)
Thromb Res. 2006;117(5):537-42. (PMID: 15951006)
Bioinformatics. 2009 Jan 15;25(2):288-9. (PMID: 19033274)
J Mol Biol. 1993 Dec 5;234(3):779-815. (PMID: 8254673)
Chemphyschem. 2009 Nov 9;10(16):2805-12. (PMID: 19784973)
Protein Sci. 2006 Nov;15(11):2507-24. (PMID: 17075131)
Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3165-70. (PMID: 17360623)
Nucleic Acids Res. 2008 Apr;36(7):2295-300. (PMID: 18287115)
J Mol Biol. 2015 Jan 16;427(1):94-105. (PMID: 25451786)
Biochem J. 2009 Nov 11;424(2):163-7. (PMID: 19811449)
Neuron. 2018 Mar 7;97(5):1063-1077.e4. (PMID: 29478917)
J Biol Chem. 2013 May 10;288(19):13305-16. (PMID: 23532839)
Physiol Rev. 2016 Apr;96(2):605-45. (PMID: 26936867)
Br J Haematol. 1967 May;13(3):269-88. (PMID: 6025241)
Nature. 2010 Dec 9;468(7325):834-8. (PMID: 21107324)
Nat Commun. 2013;4:2367. (PMID: 23996062)
PeerJ. 2014 Jun 19;2:e453. (PMID: 25024921)
IEEE Trans Pattern Anal Mach Intell. 1986 Jun;8(6):679-98. (PMID: 21869365)
Nat Genet. 2000 May;25(1):25-9. (PMID: 10802651)
Cold Spring Harb Perspect Biol. 2011 May 01;3(5):. (PMID: 21436058)
Nat Protoc. 2012 May 03;7(6):1042-51. (PMID: 22555243)
Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):3066-3071. (PMID: 29507235)
J Thromb Haemost. 2015 Dec;13(12):2240-52. (PMID: 26414452)
Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):E1667-E1674. (PMID: 29382763)
Curr Opin Struct Biol. 2016 Aug;39:61-70. (PMID: 27295354)
J Biol Chem. 1946 Nov;166(1):189-97. (PMID: 20273687)
J Cell Biol. 2013 Feb 18;200(4):373-83. (PMID: 23420871)
Proc Natl Acad Sci U S A. 2017 Jun 13;114(24):6274-6279. (PMID: 28559311)
FASEB J. 2002 Aug;16(10):1145-50. (PMID: 12153982)
J Bone Miner Res. 2013 Feb;28(2):246-59. (PMID: 22936354)
ACS Chem Biol. 2009 Nov 20;4(11):895-909. (PMID: 19689162)
PLoS One. 2009 Aug 06;4(8):e6529. (PMID: 19657394)
Proc Natl Acad Sci U S A. 2015 Oct 13;112(41):12800-5. (PMID: 26417084)
Prog Lipid Res. 2003 Sep;42(5):423-38. (PMID: 12814644)
J Biol Chem. 1989 Oct 15;264(29):17049-57. (PMID: 2793843)
Elife. 2015 Jun 09;4:e06901. (PMID: 26057829)
J Cell Sci. 1979 Feb;35:229-43. (PMID: 370129)
FEBS Lett. 2010 Jul 2;584(13):2724-30. (PMID: 20302864)
Biochim Biophys Acta. 2010 Jul;1798(7):1427-35. (PMID: 20230780)
FEBS Lett. 2010 May 3;584(9):1779-86. (PMID: 20043909)
Nature. 2017 Dec 21;552(7685):426-429. (PMID: 29236684)
Physiol Rev. 2014 Apr;94(2):419-59. (PMID: 24692353)
Blood. 2011 Apr 21;117(16):4399-400. (PMID: 21511967)
Cell Mol Life Sci. 2006 Dec;63(24):2908-21. (PMID: 17103115)
Nature. 2017 Dec 21;552(7685):421-425. (PMID: 29236691)
Mol Cell Proteomics. 2011 Dec;10(12):M111.010728. (PMID: 21937731)
Nucleic Acids Res. 2017 Jan 4;45(D1):D331-D338. (PMID: 27899567)
Sci Transl Med. 2015 Nov 25;7(315):315ra190. (PMID: 26606969)
Cell. 2012 Sep 28;151(1):111-22. (PMID: 23021219)
J Exp Med. 2016 Nov 14;213(12):2759-2772. (PMID: 27810927)
Nature. 2014 Dec 11;516(7530):207-12. (PMID: 25383531)
Cell Mol Life Sci. 2011 Aug;68(16):2667-88. (PMID: 21560073)
Science. 1976 Nov 12;194(4266):743-5. (PMID: 982044)
Biochim Biophys Acta. 2008 Jan;1778(1):20-32. (PMID: 17936718)
Biochim Biophys Acta. 2004 Mar 22;1636(2-3):119-28. (PMID: 15164759)
Elife. 2014 Jun 30;3:. (PMID: 24980701)
معلومات مُعتمدة: R01 NS069229 United States NS NINDS NIH HHS; T32 EB009383 United States EB NIBIB NIH HHS; R01 GM089740 United States GM NIGMS NIH HHS; T32 GM008568 United States GM NIGMS NIH HHS; R01 GM117593 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: GPMV; TMEM16F; calcium influx; extracellular vesicles; phospholipid scrambling
المشرفين على المادة: 0 (ANO6 protein, human)
0 (Anoctamins)
0 (Phospholipid Transfer Proteins)
0 (Phospholipids)
SY7Q814VUP (Calcium)
تواريخ الأحداث: Date Created: 20190110 Date Completed: 20190325 Latest Revision: 20231213
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC6347726
DOI: 10.1073/pnas.1817498116
PMID: 30622179
قاعدة البيانات: MEDLINE
الوصف
تدمد:1091-6490
DOI:10.1073/pnas.1817498116