دورية أكاديمية
أعرض في EDS

The Human CD4 + T Cell Response against Mumps Virus Targets a Broadly Recognized Nucleoprotein Epitope.

التفاصيل البيبلوغرافية
العنوان: The Human CD4 + T Cell Response against Mumps Virus Targets a Broadly Recognized Nucleoprotein Epitope.
المؤلفون: de Wit J; Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands jelle.de.wit@rivm.nl., Emmelot ME; Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands., Poelen MCM; Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands., Lanfermeijer J; Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands., Han WGH; Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands., van Els CACM; Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands., Kaaijk P; Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands.
المصدر: Journal of virology [J Virol] 2019 Mar 05; Vol. 93 (6). Date of Electronic Publication: 2019 Mar 05 (Print Publication: 2019).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0113724 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1098-5514 (Electronic) Linking ISSN: 0022538X NLM ISO Abbreviation: J Virol Subsets: MEDLINE
أسماء مطبوعة: Publication: Washington Dc : American Society For Microbiology
Original Publication: Baltimore, American Society for Microbiology.
مواضيع طبية MeSH: CD4-Positive T-Lymphocytes/*immunology , Epitopes, T-Lymphocyte/*immunology , Mumps/*immunology , Mumps virus/*immunology , Nucleoproteins/*immunology, HLA-DR Antigens/immunology ; Humans ; Interferon-gamma/immunology ; Leukocytes, Mononuclear/immunology
مستخلص: Mumps outbreaks among vaccinated young adults stress the need for a better understanding of mumps virus (MuV)-induced immunity. Antibody responses to MuV are well characterized, but studies on T cell responses are limited. We recently isolated a MuV-specific CD4 + T cell clone by stimulating peripheral blood mononuclear cells (PBMCs) from a mumps case with the viral nucleoprotein (MuV-N). In this study, we further explored the identity and relevance of the epitope recognized by the CD4 + T cell clone and ex vivo by T cells in a cohort of mumps cases. Using a two-dimensional matrix peptide pool of 15-mer peptides covering the complete MuV-N, we identified the epitope recognized by the T cell clone as MuV-N 110-124 GTYRLIPNARANLTA, present in a well-conserved region of the viral protein. Upon peptide-specific stimulation, the T cell clone expressed the activation marker CD137 and produced gamma interferon, tumor necrosis factor, and interleukin-10 in a HLA-DR4-restricted manner. Moreover, the CD4 + T cells exerted a cytotoxic phenotype and specifically killed cells presenting MuV-N 110-124 Furthermore, the identified peptide is widely applicable to the general population since it is predicted to bind various common HLA-DR molecules, and epitope-specific CD4 + T cells displaying cytotoxic/Th1-type properties were found in all tested mumps cases expressing different HLA-DR alleles. This first broadly recognized human MuV-specific CD4 + T cell epitope could provide a useful tool to detect and evaluate virus-specific T cell responses upon MuV infection or following vaccination. IMPORTANCE Recent outbreaks of mumps among vaccinated young adults have been reported worldwide. Humoral responses against mumps virus (MuV) are well characterized, although no correlate of protection has been elucidated, stressing the need to better understand cellular MuV-specific immunity. In this study, we identified the first MuV T cell epitope, which is derived from the viral nucleoprotein (MuV-N) and was recognized by a cytotoxic/Th1 CD4 + T cell clone that was isolated from a mumps case. Moreover, the epitope was predicted to bind a broad variety of common HLA-DRB1 alleles, which was confirmed by the epitope-specific cytotoxic/Th1 CD4 + T cell responses observed in multiple mumps cases with various HLA-DRB1 genotypes. The identified epitope is completely conserved among various mumps strains. These findings qualify this promiscuous MuV T cell epitope as a useful tool for further in-depth exploration of MuV-specific T cell immunity after natural mumps virus infection or induced by vaccination.
(Copyright © 2019 de Wit et al.)
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فهرسة مساهمة: Keywords: cytotoxicity; mumps G5 outbreak strain; mumps infection; mumps patients
المشرفين على المادة: 0 (Epitopes, T-Lymphocyte)
0 (HLA-DR Antigens)
0 (Nucleoproteins)
82115-62-6 (Interferon-gamma)
تواريخ الأحداث: Date Created: 20190111 Date Completed: 20191119 Latest Revision: 20200309
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6401470
DOI: 10.1128/JVI.01883-18
PMID: 30626672
قاعدة البيانات: MEDLINE
الوصف
تدمد:1098-5514
DOI:10.1128/JVI.01883-18