دورية أكاديمية
أعرض في EDS

Recombinant BCG strains expressing chimeric proteins derived from Leptospira protect hamsters against leptospirosis.

التفاصيل البيبلوغرافية
العنوان: Recombinant BCG strains expressing chimeric proteins derived from Leptospira protect hamsters against leptospirosis.
المؤلفون: Oliveira TL; Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil., Rizzi C; Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil., da Cunha CEP; Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil., Dorneles J; Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil., Seixas Neto ACP; Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil., Amaral MG; Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil., Hartwig DD; Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil; Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil., Dellagostin OA; Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil. Electronic address: odirad@ufpel.edu.br.
المصدر: Vaccine [Vaccine] 2019 Feb 04; Vol. 37 (6), pp. 776-782. Date of Electronic Publication: 2019 Jan 07.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8406899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2518 (Electronic) Linking ISSN: 0264410X NLM ISO Abbreviation: Vaccine Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam, The Netherlands : Elsevier Science
Original Publication: [Guildford, Surrey, UK] : Butterworths, [c1983-
مواضيع طبية MeSH: Mycobacterium bovis*, Antibodies, Bacterial/*blood , Bacterial Proteins/*immunology , Bacterial Vaccines/*immunology , Leptospira/*immunology , Leptospirosis/*prevention & control, Animals ; Bacterial Proteins/genetics ; Bacterial Vaccines/genetics ; Cricetinae ; Female ; Immunoglobulin G/blood ; Leptospira/genetics ; Male ; Recombinant Fusion Proteins/immunology ; Vaccines, Synthetic/immunology
مستخلص: Leptospirosis is a zoonosis that is responsible for one million human cases per year. Fusing multiple immunogenic antigens represents a promising approach to delivering an effective vaccine against leptospirosis. Mycobacterium bovis bacillus Calmette-Guérin (BCG) is a potential vaccine vector due to its adjuvant properties and safety. Two chimeric genes based on genic sequences of ligANI, ligBrep, lipL32, and lemA, were individually cloned into five BioBrick vectors with different promoters (pAN, Hsp60, 18 kDa, Ag85B and Ag85B plus signal sequence) for antigen expression in BCG. Groups of ten hamsters were vaccinated with recombinant BCG (rBCG) strains in two doses of 10 6 CFU and challenged with 5 × LD 50 of L. interrogans serovar Copenhageni. All rBCG vaccines expressing chimera 1, based on antigens LipL32, LigANI, and LemA, under the control of any promoter, protected 80-100% of the hamsters from challenge (P < 0.05) and four of them also protected from renal carrier status; for chimera 2, based on LigANI and LigBrep antigens, the only vaccine that afforded survival rates statistically different from the control was the vaccine that incorporated the pAN promoter (60% of survival). A single vaccine dose was sufficient to induce significant IgG levels by all vaccine compositions evaluated; however, humoral response was not related to protection. These findings suggest that the combination of potential vaccine candidates in chimeric antigens and the use of BCG as a live vector are promising strategies by which it is possible to obtain an effective and sterilizing vaccine against leptospirosis.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Bacillus Calmette-Guérin; Chimera; Leptospirosis; Recombinant vaccine
المشرفين على المادة: 0 (Antibodies, Bacterial)
0 (Bacterial Proteins)
0 (Bacterial Vaccines)
0 (Immunoglobulin G)
0 (Recombinant Fusion Proteins)
0 (Vaccines, Synthetic)
تواريخ الأحداث: Date Created: 20190112 Date Completed: 20200707 Latest Revision: 20200707
رمز التحديث: 20240628
DOI: 10.1016/j.vaccine.2018.12.050
PMID: 30630695
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2518
DOI:10.1016/j.vaccine.2018.12.050