دورية أكاديمية
أعرض في EDS

Long-term expanding human airway organoids for disease modeling.

التفاصيل البيبلوغرافية
العنوان: Long-term expanding human airway organoids for disease modeling.
المؤلفون: Sachs N; Oncode Institute, Hubrecht Institute-KNAW and UMC Utrecht, Utrecht, The Netherlands., Papaspyropoulos A; Oncode Institute, Hubrecht Institute-KNAW and UMC Utrecht, Utrecht, The Netherlands., Zomer-van Ommen DD; Wilhelmina Children's Hospital and UMC Utrecht, Utrecht, The Netherlands., Heo I; Oncode Institute, Hubrecht Institute-KNAW and UMC Utrecht, Utrecht, The Netherlands., Böttinger L; Oncode Institute, Hubrecht Institute-KNAW and UMC Utrecht, Utrecht, The Netherlands., Klay D; St. Antonius Hospital Nieuwegein, Nieuwegein, The Netherlands., Weeber F; The Netherlands Cancer Institute, Amsterdam, The Netherlands., Huelsz-Prince G; FOM Institute AMOLF, Amsterdam, The Netherlands., Iakobachvili N; Maastricht University, Maastricht, The Netherlands., Amatngalim GD; Wilhelmina Children's Hospital and UMC Utrecht, Utrecht, The Netherlands., de Ligt J; UMC Utrecht, Utrecht, The Netherlands., van Hoeck A; UMC Utrecht, Utrecht, The Netherlands., Proost N; Mouse Clinic for Cancer and Aging (MCCA) Preclinical Intervention Unit, The Netherlands Cancer Institute, Amsterdam, The Netherlands., Viveen MC; UMC Utrecht, Utrecht, The Netherlands., Lyubimova A; Oncode Institute, Hubrecht Institute-KNAW and UMC Utrecht, Utrecht, The Netherlands., Teeven L; Oncode Institute, Hubrecht Institute-KNAW and UMC Utrecht, Utrecht, The Netherlands., Derakhshan S; Wilhelmina Children's Hospital and UMC Utrecht, Utrecht, The Netherlands., Korving J; Oncode Institute, Hubrecht Institute-KNAW and UMC Utrecht, Utrecht, The Netherlands., Begthel H; Oncode Institute, Hubrecht Institute-KNAW and UMC Utrecht, Utrecht, The Netherlands., Dekkers JF; Oncode Institute, Hubrecht Institute-KNAW and UMC Utrecht, Utrecht, The Netherlands., Kumawat K; Wilhelmina Children's Hospital and UMC Utrecht, Utrecht, The Netherlands., Ramos E; Hubrecht Organoid Technology, Utrecht, The Netherlands., van Oosterhout MF; St. Antonius Hospital Nieuwegein, Nieuwegein, The Netherlands., Offerhaus GJ; UMC Utrecht, Utrecht, The Netherlands., Wiener DJ; Oncode Institute, Hubrecht Institute-KNAW and UMC Utrecht, Utrecht, The Netherlands., Olimpio EP; FOM Institute AMOLF, Amsterdam, The Netherlands., Dijkstra KK; The Netherlands Cancer Institute, Amsterdam, The Netherlands., Smit EF; The Netherlands Cancer Institute, Amsterdam, The Netherlands., van der Linden M; Wilhelmina Children's Hospital and UMC Utrecht, Utrecht, The Netherlands., Jaksani S; Hubrecht Organoid Technology, Utrecht, The Netherlands., van de Ven M; Mouse Clinic for Cancer and Aging (MCCA) Preclinical Intervention Unit, The Netherlands Cancer Institute, Amsterdam, The Netherlands., Jonkers J; Mouse Clinic for Cancer and Aging (MCCA) Preclinical Intervention Unit, The Netherlands Cancer Institute, Amsterdam, The Netherlands., Rios AC; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands., Voest EE; The Netherlands Cancer Institute, Amsterdam, The Netherlands., van Moorsel CH; St. Antonius Hospital Nieuwegein, Nieuwegein, The Netherlands., van der Ent CK; Wilhelmina Children's Hospital and UMC Utrecht, Utrecht, The Netherlands., Cuppen E; UMC Utrecht, Utrecht, The Netherlands., van Oudenaarden A; Oncode Institute, Hubrecht Institute-KNAW and UMC Utrecht, Utrecht, The Netherlands., Coenjaerts FE; UMC Utrecht, Utrecht, The Netherlands., Meyaard L; Wilhelmina Children's Hospital and UMC Utrecht, Utrecht, The Netherlands., Bont LJ; Wilhelmina Children's Hospital and UMC Utrecht, Utrecht, The Netherlands., Peters PJ; Maastricht University, Maastricht, The Netherlands., Tans SJ; FOM Institute AMOLF, Amsterdam, The Netherlands., van Zon JS; FOM Institute AMOLF, Amsterdam, The Netherlands., Boj SF; Hubrecht Organoid Technology, Utrecht, The Netherlands., Vries RG; Hubrecht Organoid Technology, Utrecht, The Netherlands., Beekman JM; Wilhelmina Children's Hospital and UMC Utrecht, Utrecht, The Netherlands., Clevers H; Oncode Institute, Hubrecht Institute-KNAW and UMC Utrecht, Utrecht, The Netherlands h.clevers@hubrecht.eu.; Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
المصدر: The EMBO journal [EMBO J] 2019 Feb 15; Vol. 38 (4). Date of Electronic Publication: 2019 Jan 14.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 8208664 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-2075 (Electronic) Linking ISSN: 02614189 NLM ISO Abbreviation: EMBO J Subsets: MEDLINE
أسماء مطبوعة: Publication: 2024- : [London] : Nature Publishing Group
Original Publication: Eynsham, Oxford, England : Published for the European Molecular Biology Organization by IRL Press, [c1982-
مواضيع طبية MeSH: Carcinoma, Non-Small-Cell Lung/*pathology , Cystic Fibrosis/*pathology , Epithelial Cells/*pathology , Organ Culture Techniques/*methods , Organoids/*pathology , Respiratory Syncytial Virus Infections/*pathology , Respiratory System/*pathology, Animals ; Carcinoma, Non-Small-Cell Lung/drug therapy ; Carcinoma, Non-Small-Cell Lung/metabolism ; Cells, Cultured ; Cystic Fibrosis/metabolism ; Cystic Fibrosis Transmembrane Conductance Regulator/metabolism ; Disease Models, Animal ; Drug Screening Assays, Antitumor ; Epithelial Cells/metabolism ; Female ; Humans ; Lung Neoplasms/drug therapy ; Lung Neoplasms/metabolism ; Lung Neoplasms/pathology ; Male ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Organoids/metabolism ; Respiratory Syncytial Virus Infections/virology ; Respiratory Syncytial Viruses/isolation & purification ; Respiratory System/metabolism ; Xenograft Model Antitumor Assays
مستخلص: Organoids are self-organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long-term-expanding human airway organoids from broncho-alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi-ciliated cells, mucus-producing secretory cells, and CC10-secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR function in an organoid swelling assay. Organoids established from lung cancer resections and metastasis biopsies retain tumor histopathology as well as cancer gene mutations and are amenable to drug screening. Respiratory syncytial virus (RSV) infection recapitulates central disease features, dramatically increases organoid cell motility via the non-structural viral NS2 protein, and preferentially recruits neutrophils upon co-culturing. We conclude that human airway organoids represent versatile models for the in vitro study of hereditary, malignant, and infectious pulmonary disease.
(© 2019 The Authors. Published under the terms of the CC BY NC ND 4.0 license.)
التعليقات: Comment in: EMBO J. 2019 Feb 15;38(4):e101526. doi: 10.15252/embj.2019101526. (PMID: 30718273)
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فهرسة مساهمة: Keywords: 3D culture; airway organoids; cystic fibrosis; lung cancer; respiratory syncytial virus
المشرفين على المادة: 0 (CFTR protein, human)
126880-72-6 (Cystic Fibrosis Transmembrane Conductance Regulator)
تواريخ الأحداث: Date Created: 20190116 Date Completed: 20200106 Latest Revision: 20240714
رمز التحديث: 20240714
مُعرف محوري في PubMed: PMC6376275
DOI: 10.15252/embj.2018100300
PMID: 30643021
قاعدة البيانات: MEDLINE
الوصف
تدمد:1460-2075
DOI:10.15252/embj.2018100300