دورية أكاديمية
أعرض في EDS

Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage.

التفاصيل البيبلوغرافية
العنوان: Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage.
المؤلفون: Aran D; Bakar Computational Health Sciences Institute, University of California, San Francisco, San Francisco, CA, USA., Looney AP; Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA., Liu L; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA, USA., Wu E; Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA., Fong V; Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA., Hsu A; Gladstone Institute of Cardiovascular Disease, San Francisco, CA, USA., Chak S; Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA., Naikawadi RP; Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA., Wolters PJ; Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA., Abate AR; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA, USA.; California Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, CA, USA.; Chan Zuckerberg Biohub, San Francisco, CA, USA., Butte AJ; Bakar Computational Health Sciences Institute, University of California, San Francisco, San Francisco, CA, USA., Bhattacharya M; Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA. mallar.bhattacharya@ucsf.edu.
المصدر: Nature immunology [Nat Immunol] 2019 Feb; Vol. 20 (2), pp. 163-172. Date of Electronic Publication: 2019 Jan 14.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature America Inc Country of Publication: United States NLM ID: 100941354 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1529-2916 (Electronic) Linking ISSN: 15292908 NLM ISO Abbreviation: Nat Immunol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, NY : Nature America Inc. c2000-
مواضيع طبية MeSH: Macrophage Activation*, Idiopathic Pulmonary Fibrosis/*immunology , Lung/*pathology , Macrophages, Alveolar/*immunology, Animals ; Antigens, Differentiation, Myelomonocytic/genetics ; Antigens, Differentiation, Myelomonocytic/immunology ; Antigens, Differentiation, Myelomonocytic/metabolism ; Bleomycin/immunology ; CX3C Chemokine Receptor 1/genetics ; CX3C Chemokine Receptor 1/immunology ; CX3C Chemokine Receptor 1/metabolism ; Cells, Cultured ; Disease Models, Animal ; Female ; Gene Expression Profiling/methods ; Humans ; Idiopathic Pulmonary Fibrosis/pathology ; Lung/cytology ; Lung/immunology ; Macrophages, Alveolar/metabolism ; Male ; Mice ; Sequence Analysis, RNA/methods ; Sialic Acid Binding Immunoglobulin-like Lectins ; Single-Cell Analysis/methods ; Up-Regulation
مستخلص: Tissue fibrosis is a major cause of mortality that results from the deposition of matrix proteins by an activated mesenchyme. Macrophages accumulate in fibrosis, but the role of specific subgroups in supporting fibrogenesis has not been investigated in vivo. Here, we used single-cell RNA sequencing (scRNA-seq) to characterize the heterogeneity of macrophages in bleomycin-induced lung fibrosis in mice. A novel computational framework for the annotation of scRNA-seq by reference to bulk transcriptomes (SingleR) enabled the subclustering of macrophages and revealed a disease-associated subgroup with a transitional gene expression profile intermediate between monocyte-derived and alveolar macrophages. These CX3CR1 + SiglecF + transitional macrophages localized to the fibrotic niche and had a profibrotic effect in vivo. Human orthologs of genes expressed by the transitional macrophages were upregulated in samples from patients with idiopathic pulmonary fibrosis. Thus, we have identified a pathological subgroup of transitional macrophages that are required for the fibrotic response to injury.
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معلومات مُعتمدة: HHSN272201200028C United States AI NIAID NIH HHS; L30 HL110167 United States HL NHLBI NIH HHS; R01 HL131560 United States HL NHLBI NIH HHS; R01 HL139897 United States HL NHLBI NIH HHS
المشرفين على المادة: 0 (Antigens, Differentiation, Myelomonocytic)
0 (CX3C Chemokine Receptor 1)
0 (CX3CR1 protein, human)
0 (Cx3cr1 protein, mouse)
0 (Sialic Acid Binding Immunoglobulin-like Lectins)
0 (Siglecf protein, mouse)
11056-06-7 (Bleomycin)
تواريخ الأحداث: Date Created: 20190116 Date Completed: 20190506 Latest Revision: 20211204
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6340744
DOI: 10.1038/s41590-018-0276-y
PMID: 30643263
قاعدة البيانات: MEDLINE
الوصف
تدمد:1529-2916
DOI:10.1038/s41590-018-0276-y