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Phosphate solubilization and acid phosphatase activity of Serratia sp. isolated from mangrove soil of Mahanadi river delta, Odisha, India.

التفاصيل البيبلوغرافية
العنوان: Phosphate solubilization and acid phosphatase activity of Serratia sp. isolated from mangrove soil of Mahanadi river delta, Odisha, India.
المؤلفون: Behera BC; Department of Biotechnology, North Orissa University, Baripada 757003, Odisha, India., Yadav H; Department of Biotechnology, Birla Institute of Scientific Research, Statue Circle, Jaipur 302001, India., Singh SK; Department of Biotechnology, Pondicherry Central University, R.V. Nagar, Kalapet, Puducherry 14, India., Mishra RR; Department of Biotechnology, MITS School of Biotechnology, Bhubaneswar 751024, India., Sethi BK; Department of Biotechnology, MITS School of Biotechnology, Bhubaneswar 751024, India., Dutta SK; Centre for Ecological Sciences, Indian Institute of Science, Bangalore 560012, India., Thatoi HN; Department of Biotechnology, North Orissa University, Baripada 757003, Odisha, India.
المصدر: Journal, genetic engineering & biotechnology [J Genet Eng Biotechnol] 2017 Jun; Vol. 15 (1), pp. 169-178. Date of Electronic Publication: 2017 Jan 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101317150 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2090-5920 (Electronic) Linking ISSN: 1687157X NLM ISO Abbreviation: J Genet Eng Biotechnol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: 2024- : [Amsterdam, Netherlands] : Elsevier B.V.
Original Publication: [Cairo] : Academy of Scientific Research and Technology, Information and Scientific Services Sector, National Information and Documentation Center
مستخلص: Phosphorus is an essential element for all life forms. Phosphate solubilizing bacteria are capable of converting phosphate into a bioavailable form through solubilization and mineralization processes. Hence in the present study a phosphate solubilizing bacterium, PSB-37, was isolated from mangrove soil of the Mahanadi river delta using NBRIP-agar and NBRIP-BPB broth containing tricalcium phosphate as the phosphate source. Based on phenotypic and molecular characterization, the strain was identified as Serratia sp. The maximum phosphate solubilizing activity of the strain was determined to be 44.84 μg/ml, accompanied by a decrease in pH of the growth medium from 7.0 to 3.15. During phosphate solubilization, various organic acids, such as malic acid (237 mg/l), lactic acid (599.5 mg/l) and acetic acid (5.0 mg/l) were also detected in the broth culture through HPLC analysis. Acid phosphatase activity was determined by performing p-nitrophenyl phosphate assay (pNPP) of the bacterial broth culture. Optimum acid phosphatase activity was observed at 48 h of incubation (76.808 U/ml), temperature of 45 °C (77.87 U/ml), an agitation rate of 100 rpm (80.40 U/ml), pH 5.0 (80.66 U/ml) and with glucose as a original carbon source (80.6 U/ml) and ammonium sulphate as a original nitrogen source (80.92 U/ml). Characterization of the partially purified acid phosphatase showed maximum activity at pH 5.0 (85.6 U/ml), temperature of 45 °C (97.87 U/ml) and substrate concentration of 2.5 mg/ml (92.7 U/ml). Hence the present phosphate solubilizing and acid phosphatase production activity of the bacterium may have probable use for future industrial, agricultural and biotechnological application.
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فهرسة مساهمة: Keywords: Bacteria; Enzyme; Mangrove ecosystem; Organic acid; Phosphatase
تواريخ الأحداث: Date Created: 20190117 Latest Revision: 20200930
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6296638
DOI: 10.1016/j.jgeb.2017.01.003
PMID: 30647653
قاعدة البيانات: MEDLINE
الوصف
تدمد:2090-5920
DOI:10.1016/j.jgeb.2017.01.003