دورية أكاديمية
Farnesol inhibits planktonic cells and antifungal-tolerant biofilms of Trichosporon asahii and Trichosporon inkin.
| العنوان: | Farnesol inhibits planktonic cells and antifungal-tolerant biofilms of Trichosporon asahii and Trichosporon inkin. |
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| المؤلفون: | Cordeiro RA; Faculty of Medicine, Post Graduate Program in Medical Microbiology, Federal University of Ceará, Brazil.; Faculty of Medicine, Post Graduate Program in Medical Sciences, Federal University of Ceará, Brazil., Pereira LMG; Faculty of Medicine, Post Graduate Program in Medical Microbiology, Federal University of Ceará, Brazil., de Sousa JK; Faculty of Medicine, Post Graduate Program in Medical Microbiology, Federal University of Ceará, Brazil., Serpa R; Faculty of Medicine, Post Graduate Program in Medical Microbiology, Federal University of Ceará, Brazil., Andrade ARC; Faculty of Medicine, Post Graduate Program in Medical Microbiology, Federal University of Ceará, Brazil., Portela FVM; Faculty of Medicine, Post Graduate Program in Medical Sciences, Federal University of Ceará, Brazil., Evangelista AJJ; Faculty of Medicine, Post Graduate Program in Medical Sciences, Federal University of Ceará, Brazil., Sales JA; Faculty of Veterinary Medicine, Post Graduate Program in Veterinary Science, State University of Ceará, Brazil., Aguiar ALR; Faculty of Medicine, Post Graduate Program in Medical Microbiology, Federal University of Ceará, Brazil., Mendes PBL; Faculty of Medicine, Post Graduate Program in Medical Microbiology, Federal University of Ceará, Brazil., Brilhante RSN; Faculty of Medicine, Post Graduate Program in Medical Microbiology, Federal University of Ceará, Brazil.; Faculty of Medicine, Post Graduate Program in Medical Sciences, Federal University of Ceará, Brazil., Sidrim JJDC; Faculty of Medicine, Post Graduate Program in Medical Microbiology, Federal University of Ceará, Brazil.; Faculty of Medicine, Post Graduate Program in Medical Sciences, Federal University of Ceará, Brazil., Castelo-Branco DSCM; Faculty of Medicine, Post Graduate Program in Medical Microbiology, Federal University of Ceará, Brazil., Rocha MFG; Faculty of Medicine, Post Graduate Program in Medical Microbiology, Federal University of Ceará, Brazil.; Faculty of Veterinary Medicine, Post Graduate Program in Veterinary Science, State University of Ceará, Brazil. |
| المصدر: | Medical mycology [Med Mycol] 2019 Nov 01; Vol. 57 (8), pp. 1038-1045. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9815835 Publication Model: Print Cited Medium: Internet ISSN: 1460-2709 (Electronic) Linking ISSN: 13693786 NLM ISO Abbreviation: Med Mycol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2014- : Oxford : Oxford University Press Original Publication: Oxford : Blackwell Science, c1998- |
| مواضيع طبية MeSH: | Antifungal Agents/*pharmacology , Biofilms/*drug effects , Farnesol/*pharmacology , Trichosporon/*drug effects , Trichosporon/*growth & development, Cell Adhesion/drug effects ; Humans ; Metabolism/drug effects ; Peptide Hydrolases/analysis ; Trichosporon/isolation & purification ; Trichosporon/metabolism ; Trichosporonosis/microbiology |
| مستخلص: | Trichosporon species have been considered important agents of opportunistic systemic infections, mainly among immunocompromised patients. Infections by Trichosporon spp. are generally associated with biofilm formation in invasive medical devices. These communities are resistant to therapeutic antifungals, and therefore the search for anti-biofilm molecules is necessary. This study evaluated the inhibitory effect of farnesol against planktonic and sessile cells of clinical Trichosporon asahii (n = 3) andTrichosporon inkin (n = 7) strains. Biofilms were evaluated during adhesion, development stages and after maturation for metabolic activity, biomass and protease activity, as well as regarding morphology and ultrastructure by optical microscopy, confocal laser scanning microscopy, and scanning electron microscopy. Farnesol inhibited Trichosporon planktonic growth by 80% at concentrations ranging from 600 to 1200 μM for T. asahii and from 75 to 600 μM for T. inkin. Farnesol was able to reduce cell adhesion by 80% at 300 μM for T. asahii and T. inkin at 600 μM, while biofilm development of both species was inhibited by 80% at concentration of 150 μM, altering their structure. After biofilm maturation, farnesol decreased T. asahii biofilm formation by 50% at 600 μM concentration and T. inkin formation at 300 μM. Farnesol inhibited gradual filamentation in a concentration range between 600 and 1200 μM. Farnesol caused reduction of filament structures of Trichosporon spp. at every stage of biofilm development analyzed. These data show the potential of farnesol as an anti-biofilm molecule. (© The Author(s) 2019. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.) |
| فهرسة مساهمة: | Keywords: Trichosporon; biofilm; farnesol; filamentation |
| المشرفين على المادة: | 0 (Antifungal Agents) 4602-84-0 (Farnesol) EC 3.4.- (Peptide Hydrolases) |
| تواريخ الأحداث: | Date Created: 20190117 Date Completed: 20200116 Latest Revision: 20200116 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1093/mmy/myy160 |
| PMID: | 30649480 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1460-2709 |
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| DOI: | 10.1093/mmy/myy160 |