دورية أكاديمية
Claudin-3-deficient C57BL/6J mice display intact brain barriers.
| العنوان: | Claudin-3-deficient C57BL/6J mice display intact brain barriers. |
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| المؤلفون: | Castro Dias M; Theodor Kocher Institute, University of Bern, Bern, Switzerland., Coisne C; Theodor Kocher Institute, University of Bern, Bern, Switzerland., Lazarevic I; Theodor Kocher Institute, University of Bern, Bern, Switzerland., Baden P; Theodor Kocher Institute, University of Bern, Bern, Switzerland., Hata M; Laboratory of Tumor Immunology and Cell Therapy, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan., Iwamoto N; Division of Cell Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan., Francisco DMF; Interfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Bern, Bern, Switzerland., Vanlandewijck M; Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre (KI/AZ ICMC), Huddinge, Sweden., He L; Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre (KI/AZ ICMC), Huddinge, Sweden., Baier FA; Visceral Surgery Research Laboratory, Department of Biomedical Research, University of Bern, Bern, Switzerland., Stroka D; Visceral Surgery Research Laboratory, Department of Biomedical Research, University of Bern, Bern, Switzerland., Bruggmann R; Interfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Bern, Bern, Switzerland., Lyck R; Theodor Kocher Institute, University of Bern, Bern, Switzerland., Enzmann G; Theodor Kocher Institute, University of Bern, Bern, Switzerland., Deutsch U; Theodor Kocher Institute, University of Bern, Bern, Switzerland., Betsholtz C; Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre (KI/AZ ICMC), Huddinge, Sweden.; Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden., Furuse M; Division of Cell Structure, National Institute for Physiological Sciences, Okazaki, Japan.; Department of Physiological Sciences, School of Life Science, Okazaki, Japan., Tsukita S; Department of Cell Biology, Kyoto University Faculty of Medicine, Kyoto, Japan., Engelhardt B; Theodor Kocher Institute, University of Bern, Bern, Switzerland. bengel@tki.unibe.ch. |
| المصدر: | Scientific reports [Sci Rep] 2019 Jan 18; Vol. 9 (1), pp. 203. Date of Electronic Publication: 2019 Jan 18. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
| مواضيع طبية MeSH: | Blood-Brain Barrier/*metabolism , Claudin-3/*metabolism , Tight Junctions/*metabolism, Animals ; Biological Transport ; Brain/metabolism ; Choroid Plexus/metabolism ; Claudin-3/genetics ; Endothelial Cells/metabolism ; Female ; Male ; Mice ; Mice, Inbred C57BL ; Tight Junction Proteins/genetics ; Tight Junction Proteins/metabolism ; Tight Junctions/genetics ; Wnt Signaling Pathway/physiology |
| مستخلص: | The tight junction protein claudin-3 has been identified as a transcriptional target of the Wnt/β-catenin signaling pathway regulating blood-brain barrier (BBB) maturation. In neurological disorders loss of claudin-3 immunostaining is observed at the compromised BBB and blood-cerebrospinal fluid barrier (BCSFB). Although these observations support a central role of claudin-3 in regulating brain barriers' tight junction integrity, expression of claudin-3 at the brain barriers has remained a matter of debate. This prompted us to establish claudin-3 -/- C57BL/6J mice to study the role of claudin-3 in brain barrier integrity in health and neuroinflammation. Bulk and single cell RNA sequencing and direct comparative qRT-PCR analysis of brain microvascular samples from WT and claudin-3 -/- mice show beyond doubt that brain endothelial cells do not express claudin-3 mRNA. Detection of claudin-3 protein at the BBB in vivo and in vitro is rather due to junctional reactivity of anti-claudin-3 antibodies to an unknown antigen still detected in claudin-3 -/- brain endothelium. We confirm expression and junctional localization of claudin-3 at the BCSFB of the choroid plexus. Our study clarifies that claudin-3 is not expressed at the BBB and shows that absence of claudin-3 does not impair brain barrier function during health and neuroinflammation in C57BL/6J mice. |
| التعليقات: | Erratum in: Sci Rep. 2019 Jul 18;9(1):10702. (PMID: 31320665) |
| References: | J Cell Biol. 1999 Nov 15;147(4):891-903. (PMID: 10562289) J Cell Biol. 1999 Dec 13;147(6):1351-63. (PMID: 10601346) Acta Neuropathol. 2000 Sep;100(3):323-31. (PMID: 10965803) Microsc Res Tech. 2001 Jan 1;52(1):112-29. (PMID: 11135454) Neurosci Lett. 2001 Jul 13;307(2):77-80. (PMID: 11427304) J Cell Biol. 2002 Mar 18;156(6):1099-111. (PMID: 11889141) Brain Pathol. 2002 Apr;12(2):154-69. (PMID: 11958369) Vascul Pharmacol. 2002 Jun;38(6):323-37. (PMID: 12529927) Acta Neuropathol. 2003 Jun;105(6):586-92. (PMID: 12734665) J Cell Biol. 2003 May 12;161(3):653-60. (PMID: 12743111) J Pathol. 2003 Oct;201(2):319-27. (PMID: 14517850) Lab Invest. 2005 Jun;85(6):734-46. (PMID: 15908914) J Immunol. 2005 Jul 15;175(2):1267-75. (PMID: 16002731) J Cell Sci. 2005 Nov 1;118(Pt 21):5109-18. (PMID: 16234325) Cell Mol Life Sci. 2006 Feb;63(4):505-14. (PMID: 16456617) Acta Neuropathol. 2007 Feb;113(2):177-86. (PMID: 17024496) Cancer Res. 2007 Sep 1;67(17):7977-82. (PMID: 17804705) J Neurochem. 2008 Jan;104(1):147-54. (PMID: 17971126) J Immunol. 2007 Dec 15;179(12):8470-9. (PMID: 18056394) Brain Res Bull. 2008 Mar 28;75(5):619-28. (PMID: 18355638) Biochim Biophys Acta. 2009 Apr;1788(4):813-9. (PMID: 18706387) J Cell Biol. 2008 Nov 3;183(3):409-17. (PMID: 18955553) J Vis Exp. 2008 Nov 10;(21):null. (PMID: 19066529) Proc Natl Acad Sci U S A. 2009 Jan 13;106(2):641-6. (PMID: 19129494) J Cereb Blood Flow Metab. 2009 Sep;29(9):1491-502. (PMID: 19491922) Acta Neuropathol. 2010 Jan;119(1):75-88. (PMID: 20033190) Proc Natl Acad Sci U S A. 2010 Apr 27;107(17):8011-6. (PMID: 20385797) J Cell Sci. 2010 Jun 1;123(Pt 11):1913-21. (PMID: 20460438) J Cereb Blood Flow Metab. 2011 Jan;31(1):315-27. (PMID: 20606687) Front Behav Neurosci. 2010 Jun 14;4:29. (PMID: 20676234) J Neuroimmunol. 2010 Dec 15;229(1-2):180-91. (PMID: 20832870) J Immunol. 2010 Oct 15;185(8):4846-55. (PMID: 20861356) PLoS One. 2010 Oct 29;5(10):e13741. (PMID: 21060791) Nat Biotechnol. 2011 Jan;29(1):24-6. (PMID: 21221095) FEBS Lett. 2011 Feb 18;585(4):606-12. (PMID: 21276448) Acta Neuropathol. 2011 Nov;122(5):601-14. (PMID: 21983942) Fluids Barriers CNS. 2012 Feb 29;9:6. (PMID: 22373538) Brain. 1990 Oct;113 ( Pt 5):1477-89. (PMID: 2245307) Fluids Barriers CNS. 2012 Aug 07;9(1):15. (PMID: 22870943) Histochem Cell Biol. 2012 Dec;138(6):861-79. (PMID: 22886143) J Neuropathol Exp Neurol. 2012 Oct;71(10):840-54. (PMID: 23001217) Acta Neuropathol. 2013 Mar;125(3):395-412. (PMID: 23269317) Genome Biol. 2013 Apr 25;14(4):R36. (PMID: 23618408) J Pharm Sci. 2013 Sep;102(9):3343-55. (PMID: 23650139) Acta Neuropathol. 2014 Aug;128(2):267-77. (PMID: 24356983) Acta Neuropathol. 2014 May;127(5):699-711. (PMID: 24429546) J Exp Med. 2014 Feb 10;211(2):233-44. (PMID: 24470444) Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404) Bioinformatics. 2015 Jan 15;31(2):166-9. (PMID: 25260700) Genome Biol. 2014;15(12):550. (PMID: 25516281) Science. 2015 Mar 6;347(6226):1138-42. (PMID: 25700174) Biomaterials. 2015 Jun;54:9-20. (PMID: 25907035) J Cell Biol. 2015 May 25;209(4):493-506. (PMID: 26008742) Bioinformatics. 2016 Jan 15;32(2):292-4. (PMID: 26428292) Fluids Barriers CNS. 2016 Jan 29;13:2. (PMID: 26833402) Front Neuroanat. 2016 Feb 22;10:16. (PMID: 26941614) Eur J Immunol. 2016 Oct;46(10):2481-2483. (PMID: 27469626) Sci Rep. 2016 Oct 11;6:35108. (PMID: 27725773) Histol Histopathol. 2017 Aug;32(8):835-849. (PMID: 27943232) Acta Neuropathol. 2017 Dec;134(6):851-868. (PMID: 28762187) Mol Psychiatry. 2018 Nov;23(11):2156-2166. (PMID: 28993710) Acta Neuropathol. 2018 Mar;135(3):337-361. (PMID: 29368213) Nature. 2018 Feb 22;554(7693):475-480. (PMID: 29443965) Brain Behav Immun. 2018 Oct;73:3-20. (PMID: 29920328) Sci Data. 2018 Aug 21;5:180160. (PMID: 30129931) J Neurosurg. 1970 Feb;32(2):127-44. (PMID: 5411991) Schriftenr Neurol. 1983;25:1-135. (PMID: 6605578) Am J Pathol. 1994 Jul;145(1):189-201. (PMID: 7518194) J Biol Chem. 1995 Apr 14;270(15):8397-400. (PMID: 7536737) J Cell Biol. 1998 Jun 29;141(7):1539-50. (PMID: 9647647) |
| المشرفين على المادة: | 0 (Claudin-3) 0 (Tight Junction Proteins) |
| تواريخ الأحداث: | Date Created: 20190120 Date Completed: 20200413 Latest Revision: 20200413 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC6338742 |
| DOI: | 10.1038/s41598-018-36731-3 |
| PMID: | 30659216 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2045-2322 |
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| DOI: | 10.1038/s41598-018-36731-3 |