دورية أكاديمية
Detection of BCR-ABL1 fusion gene transcripts in the saliva of Nigerian patients with chronic myeloid leukemia.
| العنوان: | Detection of BCR-ABL1 fusion gene transcripts in the saliva of Nigerian patients with chronic myeloid leukemia. |
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| المؤلفون: | Uzoma IC; Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria, Enugu; Department of Cell Biology and Genetics, Genetics Laboratory, Faculty of Science, University of Lagos, Lagos, Nigeria., Taiwo IA; Department of Cell Biology and Genetics, Genetics Laboratory, Faculty of Science, University of Lagos, Lagos, Nigeria., Nna EO; The Molecular Pathology Institute, Safety Molecular Pathology Laboratory, Enugu, Nigeria., Durosinmi MA; Department of Haematology, Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife, Nigeria., Ukaejiofo EO; Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria, Enugu, Nigeria. |
| المصدر: | Nigerian journal of clinical practice [Niger J Clin Pract] 2019 Jan; Vol. 22 (1), pp. 51-55. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Medknow Publications Country of Publication: India NLM ID: 101150032 Publication Model: Print Cited Medium: Internet ISSN: 1119-3077 (Print) NLM ISO Abbreviation: Niger J Clin Pract Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Mumbai : Medknow Publications Original Publication: [Lagos?] : Medical and Dental Consultants' Association of Nigeria |
| مواضيع طبية MeSH: | Saliva*, Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics , Leukemia, Myeloid, Chronic-Phase/*genetics , RNA, Messenger/*genetics, Adolescent ; Adult ; Aged ; Child ; Female ; Fusion Proteins, bcr-abl/genetics ; Humans ; Middle Aged ; Multiplex Polymerase Chain Reaction ; RNA, Messenger/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Young Adult |
| مستخلص: | Background: The presence of BCR-ABL1 fusion gene resulting from a t(9; 22) reciprocal chromosome translocation is the molecular hallmark of chronic myeloid leukemia (CML). In the diagnosis and treatment of CML, peripheral blood or bone marrow samples are usually taken for analysis. However, both methods are invasive sample collection methods, thus a noninvasive saliva sample method for the detection of the fusion gene transcripts (BCR-ABL) was investigated in some Nigerians with CML. Materials and Methods: Real-time (RT)-polymerase chain reaction (PCR) analysis was used to detect BCR-ABL1 fusion gene in the saliva and blood of 42 Nigerian CML patients. RNA was extracted using RNeasy kit and reverse transcribed by random hexamer priming using murine Moloney reverse transcriptase. BCR-ABL1 transcript types were first detected by multiplex PCR and then quantified by a duplex RT-PCR-TaqMan chemistry with MGB probe and Black Hole Quencher. Results: Of the 42 subjects, transcript types were detected in 36 (85.7%) samples, e13a2 fusion transcript sub-type was detected in 9 (21.4%), whereas e14a2 subtype was found in 27 (67.3%); six (14.3%) of the samples did not reveal any of the fusion transcript subtypes. The median BCR-ABL1 messenger RNA values were 9.38 × 10 2 in saliva and 10.29 × 10 4 in blood (P < 0.05). Similarly, the median ABL1 value in saliva (3.11 × 10 3 ) was significantly lower (P < 0.01) than in blood (4.22 × 10 3 ). However, the median BCR-ABL1 ratio in saliva (14.5%) was not significantly different (P = 0.8) from that of blood (12.0%). Conclusion: Saliva may offer an alternative easy-to-collect, readily available, and noninvasive sample for the diagnosis and treatment of CML. Competing Interests: There are no conflicts of interest. |
| فهرسة مساهمة: | Keywords: BCR-ABL1; Nigeria; Philadelphia chromosome; chronic myeloid leukemia; saliva |
| المشرفين على المادة: | 0 (BCR-ABL1 fusion protein, human) 0 (RNA, Messenger) EC 2.7.10.2 (Fusion Proteins, bcr-abl) |
| تواريخ الأحداث: | Date Created: 20190123 Date Completed: 20190130 Latest Revision: 20220331 |
| رمز التحديث: | 20240628 |
| DOI: | 10.4103/njcp.njcp_225_18 |
| PMID: | 30666020 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1119-3077 |
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| DOI: | 10.4103/njcp.njcp_225_18 |