دورية أكاديمية
Expression Profile of Long Noncoding RNAs, lnc-Cox2, and HOTAIR in Rheumatoid Arthritis Patients.
| العنوان: | Expression Profile of Long Noncoding RNAs, lnc-Cox2, and HOTAIR in Rheumatoid Arthritis Patients. |
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| المؤلفون: | Shaker OG; 1 Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Giza, Egypt., Mahmoud RH; 2 Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Abdelaleem OO; 2 Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Ahmed TI; 3 Department of Internal Medicine, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Fouad NA; 4 Department of Rheumatology and Rehabilitation, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Hussein HA; 3 Department of Internal Medicine, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Nassr MH; 4 Department of Rheumatology and Rehabilitation, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Zaki OM; 5 Department of Clinical Pathology, and Faculty of Medicine, Fayoum University, Fayoum, Egypt., Abdelghaffar NK; 5 Department of Clinical Pathology, and Faculty of Medicine, Fayoum University, Fayoum, Egypt., Hefzy EM; 6 Department of Medical Microbiology and Immunology, Faculty of Medicine, Fayoum University, Fayoum, Egypt. |
| المصدر: | Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research [J Interferon Cytokine Res] 2019 Mar; Vol. 39 (3), pp. 174-180. Date of Electronic Publication: 2019 Jan 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert Country of Publication: United States NLM ID: 9507088 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7465 (Electronic) Linking ISSN: 10799907 NLM ISO Abbreviation: J Interferon Cytokine Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Larchmont, NY : Mary Ann Liebert Original Publication: New York, NY : Mary Ann Liebert, c1995- |
| مواضيع طبية MeSH: | Arthritis, Rheumatoid/*genetics , Cyclooxygenase 2/*genetics , RNA, Long Noncoding/*genetics, Adult ; Arthritis, Rheumatoid/blood ; Biomarkers/blood ; Cyclooxygenase 2/blood ; Female ; Gene Expression Profiling ; Humans ; Interleukin-6/blood ; Male ; Matrix Metalloproteinase 9/blood ; RNA, Long Noncoding/blood |
| مستخلص: | Despite the increased proof that long noncoding RNAs (lncRNAs) can control gene expression and broadly affect the normal physiological and disease conditions, the part of lncRNAs in rheumatoid arthritis (RA) is not well known. This study aimed to assess the serum expression levels of lnc-Cox2 and HOTAIR in RA and to investigate their role as novel noninvasive biomarkers in diagnosis of RA. Also, their relations with the levels of interleukin (IL)-6 and matrix metalloproteinase (MMP)-9 and with other clinicolaboratory data in RA patients were analyzed. LncRNAs-Cox2 and HOTAIR expression levels were detected in serum by real-time quantitative polymerase chain reaction. Both IL-6 and MMP-9 levels in serum were measured by enzyme-linked immunosorbent assay. The mRNA expression of lncRNA-Cox2 and HOTAIR was significantly upregulated in RA patients compared with healthy controls. Serum levels of both IL-6 and MMP-9 were significantly higher in RA patients than in healthy subjects (P < 0.001 each). Receiver operating characteristic (ROC) curve demonstrated that lncRNA-Cox2 and HOTAIR could discriminate RA patients from healthy controls. HOTAIR (not lnc-Cox2) was observed to be an independent predictor for RA using multiple logistic regression analysis. We concluded that lnc-Cox2 and HOTAIR serum expression levels can be used as novel noninvasive biomarkers for the diagnosis of RA. |
| فهرسة مساهمة: | Keywords: HOTAIR; lnc-Cox2; rheumatoid arthritis |
| المشرفين على المادة: | 0 (Biomarkers) 0 (HOTAIR long untranslated RNA, human) 0 (Interleukin-6) 0 (RNA, Long Noncoding) EC 1.14.99.1 (Cyclooxygenase 2) EC 3.4.24.35 (Matrix Metalloproteinase 9) |
| تواريخ الأحداث: | Date Created: 20190123 Date Completed: 20200415 Latest Revision: 20200415 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1089/jir.2018.0117 |
| PMID: | 30668267 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1557-7465 |
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| DOI: | 10.1089/jir.2018.0117 |