دورية أكاديمية
Biodiversity assessments in the 21st century: the potential of insect traps to complement environmental samples for estimating eukaryotic and prokaryotic diversity using high-throughput DNA metabarcoding 1 .
| العنوان: | Biodiversity assessments in the 21st century: the potential of insect traps to complement environmental samples for estimating eukaryotic and prokaryotic diversity using high-throughput DNA metabarcoding 1 . |
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| المؤلفون: | Ritter CD; a Department of Eukaryotic Microbiology, University of Duisburg-Essen, Universitätsstrasse 5 S05 R04 H83 D-45141 Essen, Germany., Häggqvist S; b Swedish Museum of Natural History, Department of Zoology, Box 50007, 104 05 Stockholm, Sweden., Karlsson D; c Station Linné, Skogsby 161, 386 94 Färjestaden, Sweden., Sääksjärvi IE; d Biodiversity Unit, University of Turku, FI-20014 Turku, Finland., Muasya AM; e Department of Biological Sciences, University of Cape Town, Rondebosch 7701, RSA., Nilsson RH; f Gothenburg Global Biodiversity Centre, Box 461, SE-405 30 Göteborg, Sweden.; g Department of Biological and Environmental Sciences, University of Gothenburg, Box 463, 405 30 Göteborg, Sweden., Antonelli A; f Gothenburg Global Biodiversity Centre, Box 461, SE-405 30 Göteborg, Sweden.; g Department of Biological and Environmental Sciences, University of Gothenburg, Box 463, 405 30 Göteborg, Sweden.; h Gothenburg Botanical Garden, Carl Skottsbergs gata 22A, SE-41319 Göteborg, Sweden. |
| المصدر: | Genome [Genome] 2019 Mar; Vol. 62 (3), pp. 147-159. Date of Electronic Publication: 2019 Jan 23. |
| نوع المنشور: | Historical Article; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Canadian Science Publishing Country of Publication: Canada NLM ID: 8704544 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1480-3321 (Electronic) Linking ISSN: 08312796 NLM ISO Abbreviation: Genome |
| أسماء مطبوعة: | Publication: 2011- : Ottawa, ON : Canadian Science Publishing Original Publication: Ottawa, Canada : National Research Council of Canada, c1987- |
| مواضيع طبية MeSH: | Biodiversity*, DNA/*genetics , DNA Barcoding, Taxonomic/*methods , Eukaryota/*genetics , Insecta/*classification , Insecta/*genetics , Prokaryotic Cells/*metabolism, Animals ; Brazil ; DNA/analysis ; Ecosystem ; High-Throughput Nucleotide Sequencing ; History, 21st Century ; South Africa |
| مستخلص: | The rapid loss of biodiversity, coupled with difficulties in species identification, call for innovative approaches to assess biodiversity. Insects make up a substantial proportion of extant diversity and play fundamental roles in any given ecosystem. To complement morphological species identification, new techniques such as metabarcoding make it possible to quantify insect diversity and insect-ecosystem interactions through DNA sequencing. Here we examine the potential of bulk insect samples (i.e., containing many non-sorted specimens) to assess prokaryote and eukaryote biodiversity and to complement the taxonomic coverage of soil samples. We sampled 25 sites on three continents and in various ecosystems, collecting insects with SLAM traps (Brazil) and Malaise traps (South Africa and Sweden). We then compared our diversity estimates with the results obtained with biodiversity data from soil samples from the same localities. We found a largely different taxonomic composition between the soil and insect samples, testifying to the potential of bulk insect samples to complement soil samples. Finally, we found that non-destructive DNA extraction protocols, which preserve insect specimens for morphological studies, constitute a promising choice for cost-effective biodiversity assessments. We propose that the sampling and sequencing of insect samples should become a standard complement for biodiversity studies based on environmental DNA. |
| فهرسة مساهمة: | Keywords: 16S rDNA; 18S rDNA; ADN environnemental; ADNr 16S; ADNr 18S; COI ADNmt; COI mtDNA; environmental DNA; metabarcoding; métacodage à barres; non-destructive DNA extraction; protocole d’extraction d’ADN non-destructif |
| المشرفين على المادة: | 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20190124 Date Completed: 20190805 Latest Revision: 20190805 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1139/gen-2018-0096 |
| PMID: | 30673361 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1480-3321 |
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| DOI: | 10.1139/gen-2018-0096 |