دورية أكاديمية
[Clinical value of droplet digital PCR in rapid diagnosis of invasive fungal infection in neonates].
العنوان: | [Clinical value of droplet digital PCR in rapid diagnosis of invasive fungal infection in neonates]. |
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المؤلفون: | Li HT; Department of Neonatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. hwmnet@21cn.com., Lin BC, Huang ZF, Yang CZ, Huang WM |
المصدر: | Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics [Zhongguo Dang Dai Er Ke Za Zhi] 2019 Jan; Vol. 21 (1), pp. 45-51. |
نوع المنشور: | Journal Article |
اللغة: | Chinese |
بيانات الدورية: | Publisher: Zhongguo dang dai er ke za zhi she Country of Publication: China NLM ID: 100909956 Publication Model: Print Cited Medium: Print ISSN: 1008-8830 (Print) Linking ISSN: 10088830 NLM ISO Abbreviation: Zhongguo Dang Dai Er Ke Za Zhi Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Changsha : Zhongguo dang dai er ke za zhi she, 1999- |
مواضيع طبية MeSH: | Invasive Fungal Infections*, China ; Humans ; Infant, Newborn ; Polymerase Chain Reaction ; Reproducibility of Results |
مستخلص: | Objective: To evaluate the clinical value of droplet digital PCR (ddPCR) in rapid and accurate diagnosis of invasive fungal infection (IFI) in neonates. Methods: The highly conserved sequence of fungi 18S RNA was selected as the target sequence, and primers were designed to establish a ddPCR fungal detection system. Blood samples were collected from 83 neonates with high-risk factors for IFI and/or related clinical symptoms in the neonatal intensive care unit (NICU) of a hospital in Shenzhen, China. Blood culture and ddPCR were used for fungal detection. Results: The ddPCR fungal detection system had a specificity of 100% and a sensitivity of 3.2 copies/μL, and had a good reproducibility. Among the 22 blood samples from neonates with a confirmed or clinical diagnosis of IFI, 19 were detected positive by ddPCR. Among the 61 blood samples from neonates who were suspected of IFI or had no IFI, 2 were detected positive by ddPCR. Conclusions: The ddPCR technique can be used for the detection of neonatal IFI and is a promising tool for the screening and even diagnosis of neonatal IFI. |
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تواريخ الأحداث: | Date Created: 20190125 Date Completed: 20190409 Latest Revision: 20240731 |
رمز التحديث: | 20240731 |
مُعرف محوري في PubMed: | PMC7390180 |
PMID: | 30675863 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1008-8830 |
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