التفاصيل البيبلوغرافية
| العنوان: |
Sarcococca Blight: Use of Whole-Genome Sequencing for Fungal Plant Disease Diagnosis. |
| المؤلفون: |
Malapi-Wight M; United States Department of Agriculture-Agriculture Research Service (USDA-ARS), Systematic Mycology and Microbiology Laboratory, Beltsville, MD 20705., Salgado-Salazar C; USDA-ARS, Systematic Mycology and Microbiology Laboratory; and Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ 08906., Demers JE; USDA-ARS, Systematic Mycology and Microbiology Laboratory., Clement DL; University of Maryland Home and Garden Information Center, Ellicott City, MD 21042., Rane KK; Plant Diagnostic Laboratory, University of Maryland, College Park 20742., Crouch JA; USDA-ARS, Systematic Mycology and Microbiology Laboratory. |
| المصدر: |
Plant disease [Plant Dis] 2016 Jun; Vol. 100 (6), pp. 1093-1100. Date of Electronic Publication: 2016 Mar 22. |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: American Phytopathological Society Country of Publication: United States NLM ID: 9882809 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0191-2917 (Print) Linking ISSN: 01912917 NLM ISO Abbreviation: Plant Dis Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: |
Original Publication: St. Paul, Minn. : American Phytopathological Society, [1980]- |
| مستخلص: |
Early and accurate diagnosis of new plant pathogens is vital for the rapid implementation of effective mitigation strategies and appropriate regulatory responses. Most commonly, pathogen identification relies on morphology and DNA marker analysis. However, for new diseases, these approaches may not be sufficient for precise diagnosis. In this study, we used whole-genome sequencing (WGS) to identify the causal agent of a new disease affecting Sarcococca hookeriana (sarcococca). Blight symptoms were observed on sarcococca and adjacent Buxus sempervirens (boxwood) plants in Maryland during 2014. Symptoms on sarcococca were novel, and included twig dieback and dark lesions on leaves and stems. A Calonectria sp. was isolated from both hosts and used to fulfill Koch's postulates but morphology and marker sequence data precluded species-level identification. A 51.4-Mb WGS was generated for the two isolates and identified both as Calonectria pseudonaviculata. A single-nucleotide polymorphism at a noncoding site differentiated between the two host isolates. These results indicate that the same C. pseudonaviculata genotype has the ability to induce disease on both plant species. This study marks the first application of WGS for fungal plant pathogen diagnosis and demonstrates the power of this approach to rapidly identify causal agents of new diseases. |
| تواريخ الأحداث: |
Date Created: 20190126 Latest Revision: 20220331 |
| رمز التحديث: |
20231215 |
| DOI: |
10.1094/PDIS-10-15-1159-RE |
| PMID: |
30682271 |
| قاعدة البيانات: |
MEDLINE |
