التفاصيل البيبلوغرافية
| العنوان: |
Time Course of Carbendazim Stimulation on Pathogenicity of Sclerotinia sclerotiorum Indicates a Direct Stimulation Mechanism. |
| المؤلفون: |
Di YL; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China., Lu XM; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China., Zhu ZQ; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China., Zhu FX; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. |
| المصدر: |
Plant disease [Plant Dis] 2016 Jul; Vol. 100 (7), pp. 1454-1459. Date of Electronic Publication: 2016 Apr 01. |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: American Phytopathological Society Country of Publication: United States NLM ID: 9882809 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0191-2917 (Print) Linking ISSN: 01912917 NLM ISO Abbreviation: Plant Dis Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: |
Original Publication: St. Paul, Minn. : American Phytopathological Society, [1980]- |
| مستخلص: |
Previous studies have demonstrated that subtoxic doses of carbendazim have a stimulatory effect on pathogenicity of Sclerotinia sclerotiorum on rapeseed plants. The present study focused on the time-course profile of the stimulatory effect and its relevance to stimulation mechanisms. At 12 h postinoculation (HPI), initial necrotic lesions were visible only for rapeseed leaves treated with carbendazim at 0.2 and 1 μg/ml, whereas no disease symptoms were observed for the nontreated control. At 18 HPI, carbendazim stimulation on pathogenicity was more obvious than at 12 HPI. Study with scanning electron microscopy demonstrated that no discernable differences in the development of disease symptoms could be detected at 8 HPI. However, at 12 HPI, necrotic symptoms of the epidermal cells were apparent only for leaves sprayed with carbendazim. These results indicated that stimulations on pathogenicity occurred in the first 12 h, implying that direct stimulation rather than overcompensation to the disruption of homeostasis was likely to be the underlying mechanism for pathogenicity stimulation. Greenhouse experiments showed that spraying carbendazim at 400 μg/ml on potted rapeseed plants had statistically significant (P < 0.05) stimulations on pathogenicity for inoculations at 1, 3, 5, and 7 days after application (DAA). The stimulation action eventually disappeared for inoculations at 14 DAA. Mycelia grown on potato dextrose agar (PDA) supplemented with carbendazim at 400 μg/ml were more pathogenic than the nontreated control. However, after additional growth of the mycelia on fungicide-free PDA for 2 days, the stimulatory effect disappeared completely, indicating that carbendazim was indispensable for pathogenicity stimulations. Studies on biochemical mechanisms indicated that cell-wall-degrading enzymes such as cellulase, pectinase, and polygalacturonase were not involved in pathogenicity stimulations. These results will advance our understanding of the nature and mechanisms of fungicide stimulation on fungal pathogenicity and, thus, are valuable for judicious applications of fungicides. |
| تواريخ الأحداث: |
Date Created: 20190129 Latest Revision: 20191120 |
| رمز التحديث: |
20240628 |
| DOI: |
10.1094/PDIS-11-15-1349-RE |
| PMID: |
30686209 |
| قاعدة البيانات: |
MEDLINE |
