دورية أكاديمية
Isolation, Characterization and Proliferation of Cancer Cells from Breast Cancer Patients.
| العنوان: | Isolation, Characterization and Proliferation of Cancer Cells from Breast Cancer Patients. |
|---|---|
| المؤلفون: | Widowati W; Medical Research Centre, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia., Heriady Y; Faculty of Medicine, Bandung Islamic University, Bandung, Indonesia., Laksmitawati DR; Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia., Jasaputra DK; Medical Research Centre, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia., Wargasetia TL; Medical Research Centre, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia., Rizal R; Medical Research Centre, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia., Perdana FS; Biomolecular and Biomedical Research Centre, Aretha Medika Utama, Bandung, West Java, Indonesia., Amalia A; Biomolecular and Biomedical Research Centre, Aretha Medika Utama, Bandung, West Java, Indonesia., Arlisyah A; Biomolecular and Biomedical Research Centre, Aretha Medika Utama, Bandung, West Java, Indonesia., Khoiriyah Z; Biomolecular and Biomedical Research Centre, Aretha Medika Utama, Bandung, West Java, Indonesia., Faried A; Department of Neurosurgery and Stem Cell Working Group, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung, West Java, Indonesia., Subangkit M; Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia. |
| المصدر: | Acta informatica medica : AIM : journal of the Society for Medical Informatics of Bosnia & Herzegovina : casopis Drustva za medicinsku informatiku BiH [Acta Inform Med] 2018 Dec; Vol. 26 (4), pp. 240-244. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academy of Medical Sciences of Bosnia and Herzegovina Country of Publication: Bosnia and Herzegovina NLM ID: 101147064 Publication Model: Print Cited Medium: Print ISSN: 0353-8109 (Print) Linking ISSN: 03538109 NLM ISO Abbreviation: Acta Inform Med Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: Sarajevo, Bosnia and Herzegovina : Academy of Medical Sciences of Bosnia and Herzegovina Original Publication: [Sarajevo] : Society for Medical Informatics of Bosnia & Herzegovina |
| مستخلص: | Introduction: The most cases of cancer death, which are in the first rank among cancers suffered by women is breast cancer. The breast cancer therapy for patients has been done, but still not optimal, so it is necessary to understand the mechanism of therapy in model cell of breast cancer. Aim: This study aim to develop an isolation technique of breast cancer cell from patients as a cancer cell model. Material and Methods: Breast cancer cell isolation is performed by enzymatic methods using the collagen I and hyaluronidase. Then, breast cancer cells were characterized using flow cytometry based on the CD44/CD24 expression where MDA-MB468 and MCF-7 breast cancer cell lines were used as positive controls. Estrogen receptor (ER), progesterone receptor (PR), p53, HER2, and Ki67 expression were assessed using an immunohistochemistry assay. Result and Discussion: The morphology of cancer cells was fibroblast like cells on the day 7 th after isolation. Isolated breast cancer cells expressed 95.33±0.47% of CD44 + /CD24 + and human epidermal growth factor receptor 2 (HER2) low expressions. Isolation of breast cancer cells can use In-house enzymatic protocol. Isolated breast cancer showed the same expression as MDA-MB468 (CD44 + /CD24 + ) and HER2 - compared to MCF-7 cell lines (CD44 - /CD24 + ). Conclusion: These cells belonged to a basal type of breast carcinoma and expressed CD44 + /CD24 + , then isolated BCCs can be used as model cancer cells for further research. |
| References: | Methods Mol Biol. 2016;1406:121-35. (PMID: 26820950) Asian Pac J Cancer Prev. 2012;13(4):1709-10. (PMID: 22799393) Nat Protoc. 2017 Feb;12(2):439-451. (PMID: 28125105) Mod Pathol. 2011 Feb;24(2):157-67. (PMID: 21076464) Breast Cancer Res. 2011 Apr 15;13(2):R44. (PMID: 21496232) Diagn Pathol. 2007 Feb 27;2:8. (PMID: 17326824) Breast Cancer Res. 2011 Aug 12;13(4):215. (PMID: 21884641) Breast Cancer (Auckl). 2010 May 20;4:35-41. (PMID: 20697531) Front Oncol. 2011 Aug 27;1:23. (PMID: 22655234) Acta Med Okayama. 2005 Dec;59(6):253-60. (PMID: 16418768) |
| فهرسة مساهمة: | Keywords: CD24 antigen; CD44 antigen; breast cancer; cell lines; enzymatic assay |
| تواريخ الأحداث: | Date Created: 20190130 Latest Revision: 20231005 |
| رمز التحديث: | 20231005 |
| مُعرف محوري في PubMed: | PMC6311122 |
| DOI: | 10.5455/aim.2018.26.240-244 |
| PMID: | 30692706 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0353-8109 |
|---|---|
| DOI: | 10.5455/aim.2018.26.240-244 |