دورية أكاديمية
أعرض في EDS

Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides.

التفاصيل البيبلوغرافية
العنوان: Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides.
المؤلفون: Kol M; Department of Biology/Chemistry, University of Osnabrück, Osnabrück, Germany., Williams B; Department of Chemistry, Ludwig Maximilians University Munich, Munich, Germany., Toombs-Ruane H; Department of Chemistry, Ludwig Maximilians University Munich, Munich, Germany., Franquelim HG; Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Martinsried, Germany., Korneev S; Department of Biology/Chemistry, University of Osnabrück, Osnabrück, Germany., Schroeer C; Department of Biology/Chemistry, University of Osnabrück, Osnabrück, Germany., Schwille P; Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Martinsried, Germany., Trauner D; Department of Chemistry, New York University, New York, United States., Holthuis JC; Department of Biology/Chemistry, University of Osnabrück, Osnabrück, Germany., Frank JA; The Vollum Institute, Oregon Health and Science University, Portland, United States.
المصدر: ELife [Elife] 2019 Feb 05; Vol. 8. Date of Electronic Publication: 2019 Feb 05.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Cambridge, UK : eLife Sciences Publications, Ltd., 2012-
مواضيع طبية MeSH: Light*, Ceramides/*metabolism , Ceramides/*radiation effects , Sphingolipids/*biosynthesis, Complex Mixtures ; Glucosyltransferases/metabolism ; HeLa Cells ; Humans ; Transferases (Other Substituted Phosphate Groups)/metabolism ; Yeasts/enzymology
مستخلص: Ceramides are central intermediates of sphingolipid metabolism that also function as potent messengers in stress signaling and apoptosis. Progress in understanding how ceramides execute their biological roles is hampered by a lack of methods to manipulate their cellular levels and metabolic fate with appropriate spatiotemporal precision. Here, we report on clickable, azobenzene-containing ceramides, caCers, as photoswitchable metabolic substrates to exert optical control over sphingolipid production in cells. Combining atomic force microscopy on model bilayers with metabolic tracing studies in cells, we demonstrate that light-induced alterations in the lateral packing of caCers lead to marked differences in their metabolic conversion by sphingomyelin synthase and glucosylceramide synthase. These changes in metabolic rates are instant and reversible over several cycles of photoswitching. Our findings disclose new opportunities to probe the causal roles of ceramides and their metabolic derivatives in a wide array of sphingolipid-dependent cellular processes with the spatiotemporal precision of light.
Competing Interests: MK, BW, HT, HF, SK, CS, PS, DT, JH, JF No competing interests declared
(© 2019, Kol et al.)
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معلومات مُعتمدة: SFB1032 International Deutsche Forschungsgemeinschaft; SFB944 International Deutsche Forschungsgemeinschaft; Incentive Award International university of Osnabrück
فهرسة مساهمة: Keywords: HeLa cells; atomic force microscopy; biochemistry; ceramide; chemical biology; glucosylceramide; photopharmacology; sphingomyelin synthase
المشرفين على المادة: 0 (Ceramides)
0 (Complex Mixtures)
0 (Sphingolipids)
EC 2.4.1.- (Glucosyltransferases)
EC 2.4.1.80 (ceramide glucosyltransferase)
EC 2.7.8.- (Transferases (Other Substituted Phosphate Groups))
EC 2.7.8.3 (ceramide cholinephosphotransferase)
تواريخ الأحداث: Date Created: 20190206 Date Completed: 20200326 Latest Revision: 20200326
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6386522
DOI: 10.7554/eLife.43230
PMID: 30720434
قاعدة البيانات: MEDLINE
الوصف
تدمد:2050-084X
DOI:10.7554/eLife.43230