دورية أكاديمية
أعرض في EDS

Fabrication of hydrogel scaffolds via photocrosslinking of methacrylated silk fibroin.

التفاصيل البيبلوغرافية
العنوان: Fabrication of hydrogel scaffolds via photocrosslinking of methacrylated silk fibroin.
المؤلفون: Bessonov IV; JSC Efferon, Moscow, ter. Skolkovo Innovation Center, st. Nobel, 7, 121205, Russia. Department of Biology, Lomonosov Moscow State University, Leninskie Gory, 1-12, 119991, Moscow, Russia., Rochev YA, Arkhipova АY, Kopitsyna MN, Bagrov DV, Karpushkin EA, Bibikova TN, Moysenovich AM, Soldatenko AS, Nikishin II, Kotliarova MS, Bogush VG, Shaitan KV, Moisenovich MM
المصدر: Biomedical materials (Bristol, England) [Biomed Mater] 2019 Mar 14; Vol. 14 (3), pp. 034102. Date of Electronic Publication: 2019 Mar 14.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Institute of Physics Pub Country of Publication: England NLM ID: 101285195 Publication Model: Electronic Cited Medium: Internet ISSN: 1748-605X (Electronic) Linking ISSN: 17486041 NLM ISO Abbreviation: Biomed Mater Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Bristol, UK : Institute of Physics Pub.,
مواضيع طبية MeSH: Tissue Scaffolds*, Fibroins/*chemistry , Hydrogels/*chemistry, 3T3 Cells ; Actins/chemistry ; Animals ; Biocompatible Materials/chemistry ; Cell Survival ; Cross-Linking Reagents/chemistry ; Cytoskeleton/chemistry ; Formates/chemistry ; Methacrylates/chemistry ; Mice ; Microscopy, Atomic Force ; Phenotype ; Photochemistry ; Polymers/chemistry ; Propanols/chemistry ; Rheology ; Silk/chemistry ; Surface Properties ; Tissue Engineering/methods
مستخلص: Silk fibroin is a promising biomaterial for tissue engineering due to its valuable mechanical and biological properties. However, being a natural product and a protein, it lacks the processability and uniform quality of an advanced synthetic material. Here we propose a way to overcome this contradiction using novel fibroin photocrosslinkable derivative (FBMA). FBMA was synthesized by methacrylation of native fibroin nucleophilic side groups. It was dissolved in either formic acid (FA) or hexafluoroisopropanol (HFIP), and the obtained solutions were photocrosslinked into hydrogel scaffolds of various structural forms including films, micropatterns, pads and macroporous sponges. UV-exposition of dry FBMA films through a photomask created complex microscaled patterns of the polymer. The nature of the solvent affected the properties of resulting hydrogels. When HFIP was used as the solvent, the resulting hydrogels had a storage modulus ∼4 times higher than that of hydrogels fabricated using FA and ∼20 times higher compared to the reference hydrogel obtained from pristine fibroin. Both FBMA-based hydrogels were biocompatible and supported fibroblast adhesion and growth in vitro. Cells cultivated on FBMA scaffolds produced with HFIP exhibited more spread phenotype at 4 and 24 h of cultivation, consistent with increased stiffness of the hydrogel. Hence, FBMA is an attractive material for fabrication of micropatterned scaffolds of centimeter-scale size with minutely tunable physico-chemical properties via convenient and reproducible technological processes, applicable for rapid prototyping.
المشرفين على المادة: 0 (Actins)
0 (Biocompatible Materials)
0 (Cross-Linking Reagents)
0 (Formates)
0 (Hydrogels)
0 (Methacrylates)
0 (Polymers)
0 (Propanols)
0 (Silk)
0YIW783RG1 (formic acid)
3D632GYQ50 (hexafluoroisopropanol)
9007-76-5 (Fibroins)
تواريخ الأحداث: Date Created: 20190207 Date Completed: 20191021 Latest Revision: 20191022
رمز التحديث: 20231215
DOI: 10.1088/1748-605X/ab04e0
PMID: 30726780
قاعدة البيانات: MEDLINE
الوصف
تدمد:1748-605X
DOI:10.1088/1748-605X/ab04e0