دورية أكاديمية
أعرض في EDS

Establishment, molecular and biological characterization of HCB-514: a novel human cervical cancer cell line.

التفاصيل البيبلوغرافية
العنوان: Establishment, molecular and biological characterization of HCB-514: a novel human cervical cancer cell line.
المؤلفون: Rosa MN; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, SP, Brazil., Evangelista AF; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, SP, Brazil., Leal LF; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, SP, Brazil., De Oliveira CM; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, SP, Brazil., Silva VAO; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, SP, Brazil., Munari CC; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, SP, Brazil., Munari FF; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, SP, Brazil., Matsushita GM; Department of Pathology, Barretos Cancer Hospital, Barretos, SP, Brazil., Dos Reis R; Department of Gynecologic Oncology, Barretos Cancer Hospital, Barretos, SP, Brazil., Andrade CE; Department of Gynecologic Oncology, Barretos Cancer Hospital, Barretos, SP, Brazil., Souza CP; Department of Gynecologic Oncology, Barretos Cancer Hospital, Barretos, SP, Brazil., Reis RM; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, SP, Brazil. ruireis.hcb@gmail.com.; Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal. ruireis.hcb@gmail.com.; 3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal. ruireis.hcb@gmail.com.
المصدر: Scientific reports [Sci Rep] 2019 Feb 13; Vol. 9 (1), pp. 1913. Date of Electronic Publication: 2019 Feb 13.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Human papillomavirus 16*/genetics , Human papillomavirus 16*/metabolism , Neoplasm Proteins*/genetics , Neoplasm Proteins*/metabolism , Papillomavirus Infections*/genetics , Papillomavirus Infections*/metabolism , Papillomavirus Infections*/pathology , Uterine Cervical Neoplasms*/genetics , Uterine Cervical Neoplasms*/metabolism , Uterine Cervical Neoplasms*/pathology, Cell Line, Tumor ; Female ; Humans ; Exome Sequencing
مستخلص: Cervical cancer is the fourth most common cancer in women. Although cure rates are high for early stage disease, clinical outcomes for advanced, metastatic, or recurrent disease remain poor. To change this panorama, a deeper understanding of cervical cancer biology and novel study models are needed. Immortalized human cancer cell lines such as HeLa constitute crucial scientific tools, but there are few other cervical cancer cell lines available, limiting our understanding of a disease known for its molecular heterogeneity. This study aimed to establish novel cervical cancer cell lines derived from Brazilian patients. We successfully established one (HCB-514) out of 35 cervical tumors biopsied. We confirmed the phenotype of HCB-514 by verifying its' epithelial and tumor origin through cytokeratins, EpCAM and p16 staining. It was also HPV-16 positive. Whole-exome sequencing (WES) showed relevant somatic mutations in several genes including BRCA2, TGFBR1 and IRX2. A copy number variation (CNV) analysis by nanostring and WES revealed amplification of genes mainly related to kinases proteins involved in proliferation, migration and cell differentiation, such as EGFR, PIK3CA, and MAPK7. Overexpression of EGFR was confirmed by phospho RTK-array and validated by western blot analysis. Furthermore, the HCB-514 cell line was sensitive to cisplatin. In summary, this novel Brazilian cervical cancer cell line exhibits relevant key molecular features and constitutes a new biological model for pre-clinical studies.
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المشرفين على المادة: 0 (Neoplasm Proteins)
تواريخ الأحداث: Date Created: 20190215 Date Completed: 20200805 Latest Revision: 20221207
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6374403
DOI: 10.1038/s41598-018-38315-7
PMID: 30760827
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-018-38315-7