دورية أكاديمية
G1P[8] Rotavirus in children with severe diarrhea in the post-vaccine introduction era in Brazil: Evidence of reassortments and structural modifications of the antigenic VP7 and VP4 regions.
| العنوان: | G1P[8] Rotavirus in children with severe diarrhea in the post-vaccine introduction era in Brazil: Evidence of reassortments and structural modifications of the antigenic VP7 and VP4 regions. |
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| المؤلفون: | Santos FS; Programa de Pós Graduação em Biologia Parasitária da Amazônia, Universidade do Estado do Pará, Instituto Evandro Chagas, Brazil; Programa de Pós Graduação em Virologia do Instituto Evandro Chagas, Brazil., Sousa Junior EC; Programa de Pós Graduação em Virologia do Instituto Evandro Chagas, Brazil; Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Brazil., Guerra SFS; Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Brazil., Lobo PS; Programa de Pós Graduação em Virologia do Instituto Evandro Chagas, Brazil; Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Brazil., Penha Junior ET; Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Brazil., Lima ABF; Programa Institucional de Bolsas de Iniciação Científica, CNPq-Instituto Evandro Chagas, Brazil., Vinente CBG; Programa de Pós Graduação em Virologia do Instituto Evandro Chagas, Brazil., Chagas EHN; Programa de Pós Graduação em Virologia do Instituto Evandro Chagas, Brazil., Justino MCA; Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Brazil., Linhares AC; Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Brazil., Matthijnssens J; Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium., Soares LS; Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Brazil., Mascarenhas JDP; Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Brazil. Electronic address: joanamascarenhas@iec.pa.gov.br. |
| المصدر: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2019 Apr; Vol. 69, pp. 255-266. Date of Electronic Publication: 2019 Feb 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 101084138 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1567-7257 (Electronic) Linking ISSN: 15671348 NLM ISO Abbreviation: Infect Genet Evol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier Science, c2001- |
| مواضيع طبية MeSH: | Diarrhea/*epidemiology , Diarrhea/*virology , Rotavirus/*classification , Rotavirus/*genetics , Rotavirus Infections/*epidemiology , Rotavirus Infections/*virology, Antigens, Viral/chemistry ; Antigens, Viral/genetics ; Antigens, Viral/immunology ; Brazil/epidemiology ; Capsid Proteins/chemistry ; Capsid Proteins/genetics ; Capsid Proteins/immunology ; Diarrhea/prevention & control ; Genetic Variation ; Genome, Viral ; Humans ; Phylogeny ; Reassortant Viruses/genetics ; Reassortant Viruses/immunology ; Rotavirus/immunology ; Rotavirus Infections/prevention & control ; Sequence Analysis, DNA |
| مستخلص: | Worldwide rotaviruses A (RVA) are responsible for approximately 215,000 deaths annually among children aged <5 years. RVA G1P[8] remains associated with >50% of gastroenteritis cases in this age group. The aim of this study was to assess the genetic variability of G1P[8] strains detected in children with severe diarrhea in Belém, Pará, Brazil, during the post-rotavirus vaccine introduction era. Phylogenetic analysis clustered the VP4 and VP7 genes of 40 samples selected between 2009 and 2011 into lineages found to be different from the Rotarix® vaccine strain. A detailed investigation of their complete genotype constellations identified 2 reassortant viruses (5%), resulting from reassortments between the genogroups Wa-like and DS-1-like (G1-P[8]-I1-R2-C1-M1-A1-N1-T2-E1-H1) and Wa-like and AU-1-like (G1-P[8]-I1-R3-C1-M1-A1-N1-T1-E1-H1) genotype constellations. A comparison of the amino acid residues presents in the antigenic epitopes of VP7 and VP4, showed differences in the electrostatic charges distribution, between wild type Brazilian strains and the Rotarix® and RotaTeq® vaccine strains. These findings reflect the structural analyses of the antigenic regions of VP7 and VP4 of the RVA G1P[8] in children with gastroenteritis in Northern Brazil raising the hypothesis that structural modifications at these sites over time may account for the emergence of new strains that could possibly pose a challenge to current vaccines. (Copyright © 2019 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Constellations; G1P[8] genotype; Rotavirus; Vaccine |
| المشرفين على المادة: | 0 (Antigens, Viral) 0 (Capsid Proteins) 0 (VP4 protein, Rotavirus) 0 (VP7 protein, Rotavirus) |
| تواريخ الأحداث: | Date Created: 20190215 Date Completed: 20200115 Latest Revision: 20200115 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.meegid.2019.02.009 |
| PMID: | 30763774 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1567-7257 |
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| DOI: | 10.1016/j.meegid.2019.02.009 |