دورية أكاديمية
أعرض في EDS

Persistent Na + influx drives L-type channel resting Ca 2+ entry in rat melanotrophs.

التفاصيل البيبلوغرافية
العنوان: Persistent Na + influx drives L-type channel resting Ca 2+ entry in rat melanotrophs.
المؤلفون: Kayano T; Laboratory of Veterinary Physiology, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan; The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan., Sasaki Y; Laboratory of Veterinary Physiology, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan., Kitamura N; Laboratory of Veterinary Physiology, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan; The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan., Harayama N; Intensive Care Medicine, University Hospital, University of Occupational and Environmental Health, Kitakyushu, Japan., Moriya T; Laboratory of Veterinary Physiology, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan; The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan., Dayanithi G; Institut des Sciences Biologiques-Neurosciences, cognition, Centre National de la Recherche Scientifique, 3 rue Michel-Ange, Paris, France; MMDN, Institut National de la Santé et de la Recherche Médicale U1198, Université Montpellier, Montpellier, France; Ecole Pratique des Hautes Etudes-Sorbonne, Paris, France; Department of Pharmacology and Toxicology, Faculty ofMedicine, Charles University at Plzen, Plzen, Czech Republic. Electronic address: gdaya@univ-montp2.fr., Verkhratsky A; Faculty of Biology, Medicine and Health, University of Manchester, M13 9PT Manchester, UK; Achucarro Centre for Neuroscience, IKERBASQUE, Basque Foundation for Science, 48011, Bilbao, Spain., Shibuya I; Laboratory of Veterinary Physiology, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan; The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan. Electronic address: ishibuya@muses.tottori-u.ac.jp.
المصدر: Cell calcium [Cell Calcium] 2019 May; Vol. 79, pp. 11-19. Date of Electronic Publication: 2019 Feb 06.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 8006226 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-1991 (Electronic) Linking ISSN: 01434160 NLM ISO Abbreviation: Cell Calcium Subsets: MEDLINE
أسماء مطبوعة: Publication: <2003->: Amsterdam : Elsevier
Original Publication: [Edinburgh, New York] Churchill Livingston
مواضيع طبية MeSH: Calcium/*metabolism , Calcium Channels, L-Type/*metabolism , Melanotrophs/*metabolism , Sodium/*metabolism, Animals ; Male ; Patch-Clamp Techniques ; Rats ; Rats, Wistar ; Ruthenium Red/pharmacology ; TRPV Cation Channels/antagonists & inhibitors ; TRPV Cation Channels/metabolism
مستخلص: Rat melanotrophs express several types of voltage-gated and ligand-gated calcium channels, although mechanisms involved in the maintenance of the resting intracellular Ca 2+ concentration ([Ca 2+ ] i ) remain unknown. We analyzed mechanisms regulating resting [Ca 2+ ] i in dissociated rat melanotrophs by Ca 2+ -imaging and patch-clamp techniques. Treatment with antagonists of L-type, but not N- or P/Q-type voltage-gated Ca 2+ channels (VGCCs) as well as removal of extracellular Ca 2+ resulted in a rapid and reversible decrease in [Ca 2+ ] i , indicating constitutive Ca 2+ influx through L-type VGCCs. Reduction of extracellular Na + concentration (replacement with NMDG + ) similarly decreased resting [Ca 2+ ] i . When cells were champed at -80 mV, decrease in the extracellular Na + resulted in a positive shift of the holding current. In cell-attached voltage-clamp and whole-cell current-clamp configurations, the reduction of extracellular Na + caused hyperpolarisation. The holding current shifted in negative direction when extracellular K + concentration was increased from 5 mM to 50 mM in the presence of K + channel blockers, Ba 2+ and TEA, indicating cation nature of persistent conductance. RT-PCR analyses of pars intermedia tissues detected mRNAs of TRPV1, TRPV4, TRPC6, and TRPM3-5. The TRPV channel blocker, ruthenium red, shifted the holding current in positive direction, and significantly decreased the resting [Ca 2+ ] i . These results indicate operation of a constitutive cation conductance sensitive to ruthenium red, which regulates resting membrane potential and [Ca 2+ ] i in rat melanotrophs.
(Copyright © 2019. Published by Elsevier Ltd.)
فهرسة مساهمة: Keywords: Ca(2+) homeostasis; Cation conductance; Patch-clamp, Ca(2+) imaging, ruthenium red; Rat melanotrophs; Voltage-gated calcium channels
المشرفين على المادة: 0 (Calcium Channels, L-Type)
0 (TRPV Cation Channels)
11103-72-3 (Ruthenium Red)
9NEZ333N27 (Sodium)
SY7Q814VUP (Calcium)
تواريخ الأحداث: Date Created: 20190218 Date Completed: 20200721 Latest Revision: 20200721
رمز التحديث: 20231215
DOI: 10.1016/j.ceca.2019.02.001
PMID: 30772686
قاعدة البيانات: MEDLINE
الوصف
تدمد:1532-1991
DOI:10.1016/j.ceca.2019.02.001