دورية أكاديمية
أعرض في EDS

Quantitative real-time PCR for differential diagnostics of parvovirus B19 infection in acute liver failure patients.

التفاصيل البيبلوغرافية
العنوان: Quantitative real-time PCR for differential diagnostics of parvovirus B19 infection in acute liver failure patients.
المؤلفون: Alves ADR; a Laboratório de Desenvolvimento Tecnológico em Virologia , Instituto Oswaldo cruz, Fundação Oswaldo Cruz , Rio de Janeiro , Brasil., Cubel Garcia RCN; b Departamento de Microbiologia e Parasitologia , Instituto Biomédico, Universidade Federal Fluminense , Niterói , Brasil., Cruz OG; c Programa de Computação Científica , Fundação Oswaldo Cruz , Rio de Janeiro , Brasil., Pinto MA; a Laboratório de Desenvolvimento Tecnológico em Virologia , Instituto Oswaldo cruz, Fundação Oswaldo Cruz , Rio de Janeiro , Brasil., Amado Leon LA; a Laboratório de Desenvolvimento Tecnológico em Virologia , Instituto Oswaldo cruz, Fundação Oswaldo Cruz , Rio de Janeiro , Brasil.
المصدر: Expert review of molecular diagnostics [Expert Rev Mol Diagn] 2019 Mar; Vol. 19 (3), pp. 259-266. Date of Electronic Publication: 2019 Feb 26.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Validation Study
اللغة: English
بيانات الدورية: Publisher: Taylor & Francis Country of Publication: England NLM ID: 101120777 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1744-8352 (Electronic) Linking ISSN: 14737159 NLM ISO Abbreviation: Expert Rev Mol Diagn Subsets: MEDLINE
أسماء مطبوعة: Publication: 2015- : Abingdon, Oxford : Taylor & Francis
Original Publication: London : Future Drugs Ltd, 2001-
مواضيع طبية MeSH: Erythema Infectiosum/*diagnosis , Liver Failure, Acute/*diagnosis , Molecular Diagnostic Techniques/*methods , Parvovirus B19, Human/*genetics , Real-Time Polymerase Chain Reaction/*methods, Blood/virology ; DNA, Viral/genetics ; Diagnosis, Differential ; Erythema Infectiosum/complications ; Erythema Infectiosum/virology ; Humans ; Limit of Detection ; Liver/virology ; Liver Failure, Acute/etiology ; Liver Failure, Acute/virology ; Molecular Diagnostic Techniques/standards ; Parvovirus B19, Human/pathogenicity ; Real-Time Polymerase Chain Reaction/standards ; Reference Standards ; Reproducibility of Results
مستخلص: Background: Human Parvovirus B19 (B19V) is a common pathogen worldwide. After primary infection, B19V-DNA may permanently persist in non-erythroid tissues, including the liver of patients with acute liver failure (ALF).
Objective: To validate a real-time PCR (qPCR) for the quantification of B19V-DNA, in order to establish a differential diagnosis for B19V infection in ALF patients.
Methods: The qPCR techniques were based on Sybr Green® and TaqMan® methodologies. To evaluate the quality parameters of both methods, samples from patients with or without B19V infection were tested. The diagnostic utility of qPCR in the detection B19V-DNA in patients with ALF was evaluated by testing archived serum and hepatic tissue explants from 10 patients.
Results: The Sybr Green® methodology showed 97% efficiency, the limits of detection and quantification were 62.6 and 53,200 copies/mL, respectively. The TaqMan® methodology showed 95% efficiency, the limits of detection and quantification were 4.48 and 310 copies/mL, respectively. A false positive result was found only with the Sybr Green® methodology. Among ALF patients without defined etiology, three (30%) were positive for B19V DNA in serum and liver.
Conclusion: The qPCR methods validated here were effective in clarifying uncommon cases of B19V-related ALF and are fit for differential diagnosis of ALF causes.
فهرسة مساهمة: Keywords: Acute liver failure; diagnostic; human parvovirus B19; real-time quantitative PCR; synthetic stand curve
المشرفين على المادة: 0 (DNA, Viral)
تواريخ الأحداث: Date Created: 20190219 Date Completed: 20200518 Latest Revision: 20200518
رمز التحديث: 20240628
DOI: 10.1080/14737159.2019.1582333
PMID: 30773069
قاعدة البيانات: MEDLINE
الوصف
تدمد:1744-8352
DOI:10.1080/14737159.2019.1582333