دورية أكاديمية
أعرض في EDS

Action of copper(II) complex with β-diketone and 1,10-phenanthroline (CBP-01) on sarcoma cells and biological effects under cell death.

التفاصيل البيبلوغرافية
العنوان: Action of copper(II) complex with β-diketone and 1,10-phenanthroline (CBP-01) on sarcoma cells and biological effects under cell death.
المؤلفون: Polloni L; Biotechnology Institute, Federal University of Uberlândia, MG, Brazil. Electronic address: oliveirajunior@ufu.br., Seni Silva AC; Biotechnology Institute, Federal University of Uberlândia, MG, Brazil., Teixeira SC; Department of Immunology, Biomedical Sciences Institute, Federal University of Uberlândia, MG, Brazil., Azevedo FVPV; Biotechnology Institute, Federal University of Uberlândia, MG, Brazil., Zóia MAP; Biotechnology Institute, Federal University of Uberlândia, MG, Brazil., da Silva MS; Center of Toxins, Immune Response and Cell Signaling (CeTICS), Butantan Institute, São Paulo, SP, Brazil; The Wellcome Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom., Lima PMAP; Biotechnology Institute, Federal University of Uberlândia, MG, Brazil., Correia LIV; Biotechnology Institute, Federal University of Uberlândia, MG, Brazil., do Couto Almeida J; Chemistry Institute, Federal University of Uberlândia, MG, Brazil., da Silva CV; Department of Immunology, Biomedical Sciences Institute, Federal University of Uberlândia, MG, Brazil., Rodrigues Ávila VM; Biotechnology Institute, Federal University of Uberlândia, MG, Brazil., Goulart LRF; Biotechnology Institute, Federal University of Uberlândia, MG, Brazil., Morelli S; Biotechnology Institute, Federal University of Uberlândia, MG, Brazil., Guerra W; Chemistry Institute, Federal University of Uberlândia, MG, Brazil., Oliveira Júnior RJ; Biotechnology Institute, Federal University of Uberlândia, MG, Brazil. Electronic address: robson_jurn@yahoo.com.br.
المصدر: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2019 Apr; Vol. 112, pp. 108586. Date of Electronic Publication: 2019 Feb 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Editions Scientifiques Elsevier Country of Publication: France NLM ID: 8213295 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1950-6007 (Electronic) Linking ISSN: 07533322 NLM ISO Abbreviation: Biomed Pharmacother Subsets: MEDLINE
أسماء مطبوعة: Publication: Paris : Editions Scientifiques Elsevier
Original Publication: New York, N.Y. : Masson Pub. USA, Inc., c1982-
مواضيع طبية MeSH: Cell Survival/*drug effects , Chelating Agents/*administration & dosage , Copper/*administration & dosage , Phenanthrolines/*administration & dosage , Sarcoma 180/*metabolism, Animals ; Cell Death/drug effects ; Cell Death/physiology ; Cell Line, Tumor ; Cell Survival/physiology ; Chelating Agents/chemistry ; Copper/chemistry ; Dose-Response Relationship, Drug ; Mice ; Phenanthrolines/chemistry ; Sarcoma 180/drug therapy ; Sarcoma 180/pathology
مستخلص: This work reports the biological evaluation of a copper complex of the type [Cu(O-O)(N-N)ClO 4 ], in which O-O = 4,4,4-trifluoro-1-phenyl-1,3-butanedione (Hbta) and N-N = 1,10-phenanthroline (phen), whose generic name is CBP-01. The cytotoxic effect of CBP-01 was evaluated by resazurin assay and cell proliferation was determined by MTT assay. DNA fragmentation was analyzed by gel electrophoresis. Cell cycle progression was detected through propidium iodide (PI) staining. Apoptosis and autophagy were determined by, respectively, Annexin V and 7-AAD staining and monodansylcadaverine (MDC) staining. The changes in intracellular reactive oxygen species levels were detected by DCFDA analysis. The copper complex CBP-01 showed in vitro antitumor activity with IC 50 s values of 7.4 μM against Sarcoma 180 and 26.4 against murine myoblast cells, displaying selectivity toward the tumor cell tested in vitro (SI > 3). An increase in reactive oxygen species (ROS) generation was observed, which may be related to the action mechanism of the complex. The complex CBP-01 may induce DNA damage leading cells to accumulate at G0/G1 checkpoint where, apparently, cells that are not able to recover from the damage are driven to cell death. Evidence has shown that cell death is initiated by autophagy dysfunction, culminating in apoptosis induction. The search for new metal-based drugs is focused on overcoming the drawbacks of already used agents such as acquired resistance and non-specificity; thus, the results obtained with CBP-01 show promising effects on cancer cells.
(Copyright © 2019 Elsevier Masson SAS. All rights reserved.)
فهرسة مساهمة: Keywords: Apoptosis; Cell cycle; Copper complex; Cytotoxicity; ROS
المشرفين على المادة: 0 (Chelating Agents)
0 (Phenanthrolines)
789U1901C5 (Copper)
W4X6ZO7939 (1,10-phenanthroline)
تواريخ الأحداث: Date Created: 20190221 Date Completed: 20190722 Latest Revision: 20190722
رمز التحديث: 20231215
DOI: 10.1016/j.biopha.2019.01.047
PMID: 30784909
قاعدة البيانات: MEDLINE
الوصف
تدمد:1950-6007
DOI:10.1016/j.biopha.2019.01.047