دورية أكاديمية
أعرض في EDS

Scaled-Up Inertial Microfluidics: Retention System for Microcarrier-Based Suspension Cultures.

التفاصيل البيبلوغرافية
العنوان: Scaled-Up Inertial Microfluidics: Retention System for Microcarrier-Based Suspension Cultures.
المؤلفون: Moloudi R; School of Mechanical and Aerospace Engineering, Nanyang Technological University (NTU), 50 Nanyang Avenue, Singapore, 639798.; Bio-Manufacturing Programme, Singapore Institute of Manufacturing Technology (SIMTech), Agency for Science, Technology and Research (A*STAR), Innovis, Singapore, 138634., Oh S; Stem Cell Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Centros, Singapore, 138668., Yang C; School of Mechanical and Aerospace Engineering, Nanyang Technological University (NTU), 50 Nanyang Avenue, Singapore, 639798., Teo KL; Stem Cell Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Centros, Singapore, 138668., Lam AT; Stem Cell Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Centros, Singapore, 138668., Ebrahimi Warkiani M; School of Biomedical Engineering, Center for Health Technologies, University of Technology Sydney, Ultimo, Sydney, NSW, 2007, Australia.; Institute of Molecular Medicine, Sechenov University, Moscow, Russia, 119146., Win Naing M; Bio-Manufacturing Programme, Singapore Institute of Manufacturing Technology (SIMTech), Agency for Science, Technology and Research (A*STAR), Innovis, Singapore, 138634.
المصدر: Biotechnology journal [Biotechnol J] 2019 May; Vol. 14 (5), pp. e1800674. Date of Electronic Publication: 2019 Apr 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley-VCH Verlag Country of Publication: Germany NLM ID: 101265833 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1860-7314 (Electronic) Linking ISSN: 18606768 NLM ISO Abbreviation: Biotechnol J Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Weinheim : Wiley-VCH Verlag, c2006-
مواضيع طبية MeSH: Cell Culture Techniques/*methods , Microfluidic Analytical Techniques/*methods , Microfluidics/*methods, Biological Phenomena ; Bone Marrow Cells ; Cell Culture Techniques/instrumentation ; Cell Separation/instrumentation ; Cell Separation/methods ; Flow Cytometry ; Humans ; Mesenchymal Stem Cells ; Microfluidic Analytical Techniques/instrumentation ; Microfluidics/instrumentation ; Particle Size
مستخلص: Recently, particle concentration and filtration using inertial microfluidics have drawn attention as an alternative to membrane and centrifugal technologies for industrial applications, where the target particle size varies between 1 µm and 500 µm. Inevitably, the bigger particle size (>50 µm) mandates scaling up the channel cross-section or hydraulic diameter (D H > 0.5 mm). The Dean-coupled inertial focusing dynamics in spiral microchannels is studied broadly; however, the impacts of secondary flow on particle migration in a scaled-up spiral channel is not fully elucidated. The mechanism of particle focusing inside scaled-up rectangular and trapezoidal spiral channels (i.e., 5-10× bigger than conventional microchannels) with an aim to develop a continuous and clog-free microfiltration system for bioprocessing is studied in detail. Herein, a unique focusing based on inflection point without the aid of sheath flow is reported. This new focusing mechanism, observed in the scaled-up channels, out-performs the conventional focusing scenarios in the previously reported trapezoidal and rectangular channels. Finally, as a proof-of-concept, the utility of this device is showcased for the first time as a retention system for a cell-microcarrier (MC) suspension culture.
(© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
معلومات مُعتمدة: U18-B-017SU SIMT/18-410006 Singapore Institute of Manufacturing Technology; DP170103704 Australian Research Council via Discovery Project
فهرسة مساهمة: Keywords: inertial microfluidics; mesenchymal stem cells; microcarrier; retention systems; scale-up; secondary flow
تواريخ الأحداث: Date Created: 20190222 Date Completed: 20191024 Latest Revision: 20191024
رمز التحديث: 20221213
DOI: 10.1002/biot.201800674
PMID: 30791214
قاعدة البيانات: MEDLINE
الوصف
تدمد:1860-7314
DOI:10.1002/biot.201800674