دورية أكاديمية
أعرض في EDS

Semi-quantitative duplex RT-PCR reveals the low occurrence of Porcine Pegivirus and Atypical Porcine Pestivirus in diagnostic samples from the United States.

التفاصيل البيبلوغرافية
العنوان: Semi-quantitative duplex RT-PCR reveals the low occurrence of Porcine Pegivirus and Atypical Porcine Pestivirus in diagnostic samples from the United States.
المؤلفون: Chen F; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota.; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China., Knutson TP; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota., Braun E; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota., Jiang Y; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota., Rossow S; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota., Marthaler DG; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota.; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas.
المصدر: Transboundary and emerging diseases [Transbound Emerg Dis] 2019 May; Vol. 66 (3), pp. 1420-1425. Date of Electronic Publication: 2019 Mar 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Blackwell Verlag Country of Publication: Germany NLM ID: 101319538 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1865-1682 (Electronic) Linking ISSN: 18651674 NLM ISO Abbreviation: Transbound Emerg Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin : Blackwell Verlag
مواضيع طبية MeSH: Metagenomics*, Coinfection/*veterinary , Flaviviridae/*isolation & purification , Flaviviridae Infections/*veterinary , Swine Diseases/*epidemiology, Animals ; Flaviviridae/genetics ; Flaviviridae Infections/epidemiology ; Flaviviridae Infections/virology ; Minnesota/epidemiology ; Pestivirus/genetics ; Pestivirus/isolation & purification ; Pestivirus Infections/epidemiology ; Pestivirus Infections/veterinary ; Pestivirus Infections/virology ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction/veterinary ; Swine ; Swine Diseases/virology ; United States/epidemiology
مستخلص: Porcine Pegivirus (PPgV) and Atypical Porcine Pestivirus (APPV) are two recently identified porcine viruses. In this study, the identification of two viruses by metagenomic sequencing, and a duplex semi-quantitative RT-PCR was developed to detect these pathogens simultaneously. The PPgV strain Minnesota-1/2016 had a 95.5%-96.3% nucleotide identity and clustered with the recently identified US PPgV strains, which is a distant clade from the German PPgV strains. The APPV strain Minnesota-1/2016 shared an 87.3%-92.0% nucleotide identity with the other global APPV strains identity but only shared an 82.8%-83.0% nucleotide identity with clade II consisting of strain identified in China. Detection of both PPgV and APPV was 9.0% of the diagnostic cases. Co-infection of PPgV and APPV was identified in 7.5% of the diagnostic cases. The occurrence and genetic characterization of PPgV and APPV further enhance our knowledge regarding these new pathogens in the United States.
(© 2019 The Authors. Transboundary and Emerging Diseases published by Blackwell Verlag GmbH.)
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معلومات مُعتمدة: CARS-36 China Agriculture Research System; 201606760012 China Scholarship Council; Hatch 1013569 National Institute of Food and Agriculture
فهرسة مساهمة: Keywords: Pegivirus; atypical porcine pestivirus; phylogenetics
سلسلة جزيئية: GENBANK KU351669; KU351671; KY798013; KY624591; KX929062; KX778724; KU041639; KY612413; KX950762; KX950761; KY652092; LT594521; KU194229; KR011347; MF590069
تواريخ الأحداث: Date Created: 20190227 Date Completed: 20190830 Latest Revision: 20210109
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6849716
DOI: 10.1111/tbed.13154
PMID: 30806022
قاعدة البيانات: MEDLINE
الوصف
تدمد:1865-1682
DOI:10.1111/tbed.13154