دورية أكاديمية
Oral fluid for detection of exposure to Lawsonia intracellularis in naturally infected pigs.
| العنوان: | Oral fluid for detection of exposure to Lawsonia intracellularis in naturally infected pigs. |
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| المؤلفون: | Barrera-Zarate JA; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31.270-901, Brazil., Andrade MR; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31.270-901, Brazil., Pereira CER; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31.270-901, Brazil., Vasconcellos A; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31.270-901, Brazil., Wagatsuma MM; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31.270-901, Brazil., Sato JPH; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31.270-901, Brazil., Daniel AGS; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31.270-901, Brazil., Rezende LA; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31.270-901, Brazil., Otoni LAV; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31.270-901, Brazil., Laub RP; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31.270-901, Brazil., Macedo NR; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31.270-901, Brazil., Costa CM; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31.270-901, Brazil., Haddad JPA; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31.270-901, Brazil., Guedes RMC; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31.270-901, Brazil. Electronic address: guedes@vet.ufmg.br. |
| المصدر: | Veterinary journal (London, England : 1997) [Vet J] 2019 Feb; Vol. 244, pp. 34-36. Date of Electronic Publication: 2018 Dec 07. |
| نوع المنشور: | Evaluation Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Balliere Tindall Country of Publication: England NLM ID: 9706281 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2971 (Electronic) Linking ISSN: 10900233 NLM ISO Abbreviation: Vet J Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Balliere Tindall Original Publication: London : Ballière Tindall, c1997- |
| مواضيع طبية MeSH: | Animal Husbandry*, Desulfovibrionaceae Infections/*veterinary , Lawsonia Bacteria/*isolation & purification , Swine Diseases/*epidemiology, Animals ; Brazil/epidemiology ; Desulfovibrionaceae Infections/diagnosis ; Desulfovibrionaceae Infections/epidemiology ; Feces/microbiology ; Female ; Male ; Saliva/microbiology ; Specimen Handling/veterinary ; Swine ; Swine Diseases/diagnosis ; Swine Diseases/microbiology |
| مستخلص: | To demonstrate the utility of oral fluid (OF) for indirect diagnostic detection of Lawsonia intracellularis (Li), 15 pig farms were studied. Serum and fecal samples were collected from 20 animals from five different age groups on each farm. OF samples were collected from animals in two pens of the same age groups. Serum and OF samples were analyzed in an immunoperoxidase in monolayer assay (IPMA) for the detection of anti-Li immunoglobulin G (IgG) and A (IgA). Compatible results were found between PCR and IgG in OF in four of the five ages evaluated. Simultaneous detection of IgG in serum and OF was mainly observed on farms showing clinical signs suggestive of porcine proliferative enteropathy (PPE). These findings demonstrate the potential usefulness of OF in detecting anti-Li antibodies as a diagnostic tool that can be used to monitor PPE in herds with clinical signs compatible with the disease. (Copyright © 2018. Published by Elsevier Ltd.) |
| فهرسة مساهمة: | Keywords: Antibodies; IPMA; Oral fluid; Porcine proliferative enteropathy; Serology |
| تواريخ الأحداث: | Date Created: 20190304 Date Completed: 20190321 Latest Revision: 20191210 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.tvjl.2018.12.003 |
| PMID: | 30825892 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1532-2971 |
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| DOI: | 10.1016/j.tvjl.2018.12.003 |