دورية أكاديمية
Evaluation of different strategies to promote a protective immune response against leptospirosis using a recombinant LigA and LigB chimera.
| العنوان: | Evaluation of different strategies to promote a protective immune response against leptospirosis using a recombinant LigA and LigB chimera. |
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| المؤلفون: | da Cunha CEP; Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Brazil., Bettin EB; Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Brazil., Bakry AFAAY; Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Brazil., Seixas Neto ACP; Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Brazil., Amaral MG; Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Brazil., Dellagostin OA; Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Brazil. Electronic address: odir@ufpel.edu.br. |
| المصدر: | Vaccine [Vaccine] 2019 Mar 22; Vol. 37 (13), pp. 1844-1852. Date of Electronic Publication: 2019 Feb 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8406899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2518 (Electronic) Linking ISSN: 0264410X NLM ISO Abbreviation: Vaccine Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam, The Netherlands : Elsevier Science Original Publication: [Guildford, Surrey, UK] : Butterworths, [c1983- |
| مواضيع طبية MeSH: | Antigens, Bacterial/*immunology , Bacterial Vaccines/*immunology , Leptospira/*immunology , Leptospirosis/*prevention & control , Recombinant Fusion Proteins/*immunology , Vaccines, Subunit/*immunology, Adjuvants, Immunologic ; Animals ; Antibodies, Bacterial/immunology ; Antigens, Bacterial/genetics ; Bacterial Vaccines/genetics ; CHO Cells ; Cricetinae ; Cricetulus ; Gene Expression ; Humans ; Leptospira/genetics ; Leptospirosis/immunology ; Leptospirosis/microbiology ; Leptospirosis/mortality ; Recombinant Fusion Proteins/genetics ; Vaccines, DNA ; Vaccines, Subunit/genetics |
| مستخلص: | Leptospirosis is a zoonosis of worldwide distribution, caused by infection with pathogenic Leptospira species. The vaccines that are currently available are bacterins, with limited human use, that confer short-term, serovar-specific immunity. Lig proteins are considered to be the best vaccine candidates to date. Here, we aimed to construct a recombinant Lig chimera (LC) comprised of LigAni and LigBrep fragments, and to evaluate it as subunit or DNA vaccine using different administration strategies. Vaccines were formulated with 50 µg of recombinant LC associated with different adjuvants or with 100 µg of pTARGET/LC. Four-week-old hamsters received two doses of vaccine with different strategies and were challenged with 5 × DL (Copyright © 2019 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: DNA vaccine; Leptospira; Leptospiral Immunoglobulin-like; Protection; Subunit vaccine |
| المشرفين على المادة: | 0 (Adjuvants, Immunologic) 0 (Antibodies, Bacterial) 0 (Antigens, Bacterial) 0 (Bacterial Vaccines) 0 (LigA-m protein, Leptospira interrogans) 0 (LigB-m protein, Leptospira interrogans) 0 (Recombinant Fusion Proteins) 0 (Vaccines, DNA) 0 (Vaccines, Subunit) |
| تواريخ الأحداث: | Date Created: 20190304 Date Completed: 20200812 Latest Revision: 20200812 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.vaccine.2019.02.010 |
| PMID: | 30826147 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1873-2518 |
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| DOI: | 10.1016/j.vaccine.2019.02.010 |